12. Protein post-translationally modified have distinct roles:

13. Protein isoforms from a single gene have different physiological vs. pathological functions

14. Cleavage products have functions distinct from the parent protein

15. Example: APP protein Abeta peptides, soluble APP, AICD

16. Proteins are assembled into protein complexes, and these are affected by isoform and post-translational modification

17. Example: Tau protein: how many microtubule binding domains (which exons used from gene), phosphorylation state of protein affect MT binding or fibril formation

20. Multiple splice variant mRNAs

21. Mulitiple protein isoforms expressed

22. Some isoforms are ubiquitous, others neuron specific or T cell-specific

23. Variety of conserved functional domains throughout length of protein

24. Isoform differences in major domains may have functional consequences

25. APP interacts with multiple proteins

26. Mutations throughout protein associated with familial Alzheimer Disease

30. Isoforms

31. Splice variants

32. Cleavage products

33. Mutations

34. Protein complexes: homomultimeric and heteromultimeric

35. Tissue-specific expression, protein complexes are important

37. a cleavage product of APP

38. a mutated form of APP

49. Essential fields from RACE-PRO

50. “Tracer ID” tracks individual rows

51. Supports automated terms management processes

55. Java application allows curators to review, reject, or extend annotations

56. Annotations exported to an OBI RDF format

60. Timothy Danford

61. Tim Clark

63. Jonathan Rees

65. Gwen Wong