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Avian Salmonella Inactivated Vaccine Against Probiotics

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"عسى أن تكون علما ينتفع به"

Avian Salmonella Inactivated Vaccine Against Probiotics

  1. 1. Salmonella Bacterin Against Probiotic Salmonella Enteritidis Infection in Broiler
  2. 2. Trial Aim This study was carried out to investigate the efficacy of the locally prepared autogenous Salmonella Enteritidis (S. Enteritidis) bacterin as well as a probiotic preparation in the prevention of broiler chickens from S. Enteritidis infection.
  3. 3. Experimental Chickens 310, day-old Hubbard broiler chicks of mixed sex. Obtained from Cairo Poultry Company in 10th Ramadan city. Breeder flock free from salmonellosis.
  4. 4. Experimental Design  At arrival, randomly 10 chicks were sacrificed and then examined bacteriologically to prove their freedom from S. Enteritidis infection.
  5. 5. Experimental Chickens The birds were kept under complete observation for six weeks experimental period) in separate thoroughly cleaned and disinfected houses.
  6. 6. Vaccination Program ND IB AI IBD Day 6: HB1 Day 19: L aSota Day 6: H120 Day 7: H5N1 RG Day 14: 228E
  7. 7. Salmonella Vaccination Program  Inactivated S. Enteritidis bacterin was given IM in the thigh muscle for the experimental chicks at: 1. First day of age in a dose of 0.2 ml/bird 2. Second dose at 10 days of age in a dose 0.5 ml/bird.
  8. 8. The Used Probiotic  Commercial preparation containing: – (Lactobacillus acidophilus, Enterococcus faecium, Lactobacillus plantarum and Lactobacillus casei) – Potassium – Vitamins A, D3, E and K, – Riboflavin, pantothenic acid, thiamine and niacinamide.  That product was manufactured by Bomac Vets Plus, USA. Batch No., A704.  It was given in the drinking water at the age of one day for 5 consecutive days in a dose of 1gm/ 4 liter of the drinking water as recommended by manufacturer.
  9. 9. The Challenge Inoculum  Broth culture of S. Enteritidis field strain was centrifuged at 3000 r.p.m for 10 min.  Sediment was diluted with sterile buffer saline and adjusted using Mac Ferland matching tube to contain 10 log 9 CFU/ml.  The challenge inoculum was prepared according to the method of Timms et al., (1990).  At 20 days of age, each bird in the experimentally infected groups was inoculated orally with 0.5 ml/ containing 10 log 9 CFU/ml S. Enteritidis (Okamoto et al., 2007).
  10. 10. 4 Groups, each of 75 chicks: Non-Vaccinated Non-Challenged 1 Infected Not Treated 2 Vaccinated Infected 3 Probiotic Infected 4
  11. 11. Evaluation 1. Clinical Signs 2. Mortalities 3. Performance 4. Gross Lesions 5. Detection of the Shedding of S. Enteritidis Birds in the challenged groups were observed daily for three weeks post challenge till the end of the study (6 weeks of age) for the clinical signs or deaths. Dead birds were subjected to necropsy for recording the lesions of S. Enteritidis (O'Brien 1988).
  12. 12. Cont. … Detection of the Shedding of S. Enteritidis  Cloacal swabs were taken from birds in each group just before experimental infection (at 20 days of age) to ensure that the birds free from S. Enteritidis infection.  Weekly after the challenge up to 6 weeks of age, cloacal swabs were collected from each of the infected as well as control group and examined bacteriologically for the presence of S. Enteritidis organism.  Sterile cotton swab was inserted into the cloaca of each bird and rotated gently to collect the clocal contents.  Each swab was transferred to 10 ml tube of tetrathionate broth and incubated overnight at 37°C.  A loopful from the broth was streaked on S.S agar for Salmonella isolation.  Suspected colonies were identified morphologically and biochemically.
  13. 13. Cont. … Re-isolation of S. Enteritidis  Ten birds from each group post challenge were weekly randomly selected, sacrificed and the liver, heart, spleen and caecum were collected for S. Enteritidis re-isolation.  Samples were inoculated into tetrathionate broth, incubated at 37°C for 24 hr, streaked onto S.S agar and incubated at 37°C for 24 hr. Suspected colonies were identified morphologically and biochemically.
  14. 14. Cont. … Performance At arrival, the chicks were weighed and then the birds in each group were subjected to weekly determination of the production parameters that include; 1. Body weight (BW) 2. Cumulative feed conversion (CFC) 3. European production efficiency factor (EPEF) according to sainsbury (1984). These measures were taken till the end of the study (6 weeks of age).
  15. 15. Results Control Non-treated - Infected Vaccinated - Infected Probiotic - Infected 0 23 4 9 Number of Dead Birds
  16. 16. Results Control Non-treated - Infected Vaccinated - Infected Probiotic - Infected 0% 31% 5% 12% Mortality Rate %
  17. 17. Results Control Non-treated - Infected Vaccinated - Infected Probiotic - Infected 0% 52% 18% 26% SE Faecal Shedding Week 1
  18. 18. Results Control Non-treated - Infected Vaccinated - Infected Probiotic - Infected 0% 37% 7% 18% SE Faecal Shedding Week 2
  19. 19. Results Control Non-treated - Infected Vaccinated - Infected Probiotic - Infected 0% 25% 0% 4% SE Faecal Shedding Week 3
  20. 20. Results Control Non-treated - Infected Vaccinated - Infected Probiotic - Infected 0% 41% 9% 18% SE Faecal Shedding Total SE Faecal Shedding Total
  21. 21. Results Control Non-treated - Infected Vaccinated - Infected Probiotic - Infected 0% 47% 12% 22% Re-isolation rate of S. Enteritidis from different organs
  22. 22. Results Control Non-treated - Infected Vaccinated - Infected Probiotic - Infected 2.03 2.43 1.78 1.89 Cumulative Feed Conversion
  23. 23. Results Control Non-treated - Infected Vaccinated - Infected Probiotic - Infected 1,580 1,355 1,705 1,640 Average body weight after 6 weeks
  24. 24. Conclusion From this study, it could be concluded that both the locally prepared autogenous S. Enteritidis bacterin in double doses and the probiotic preparation are effective and safe methods for prevention of S. Enteritidis infection in broiler chickens.
  • ShubhamNandanwar2

    Apr. 10, 2021
  • MohamedFekry45

    Dec. 20, 2017
  • rodro90

    Oct. 10, 2017
  • MohamedFouadElganayni

    Sep. 1, 2016
  • ahmedelbedwahy

    Apr. 28, 2016
  • cell_Ehsan

    Apr. 28, 2015
  • snipervet16

    Apr. 26, 2015
  • sameheshorbagy

    Dec. 8, 2014

"عسى أن تكون علما ينتفع به"

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