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Group 6 physiology


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This slide is a result of colaboration of my group in our practicum during the semester break.....

its include a lot of simple basic things in physiology...

-animal handling
-blood grouping
-vaginal swab in female rats
-breathing n ECG

hope this will contribute something!

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Group 6 physiology

  2. 2. Day 1 Handling & Administration of Substances into Rats & Mice
  3. 3. Handling of rats and mice One-hand method Scruff the body with one hand while another hand securing the tail. Scruff at the back of the neck and secure tail with same hand. Two-hand method
  4. 4. Administration of substances Intraperitoneal Intramuscular Intraplantar SubcutaneousIntravenous Intraarterial Oral gavage
  5. 5. Intraperitoneal Restrain well. Insert needle at 30- degree angle. Aspirate. If there is no fluid aspirated, continue the injection.
  6. 6. Oral gavage Measure needle length to last rib. Scruff firmly and hold head in vertical alignment with esophagus. Insert needle tip behind incisors, down into esophagus. There should be no resistance if the needle is inserted into the esophagus.
  7. 7. Oral gavage performed on rat.
  8. 8. Subcutaneous Lift skin over the neck to form a tent-like shape. Insert the needle carefully at the tent base. Aspirate to ensure the needle do not enter blood vessel. Inject the full volume at moderate rate
  9. 9. Intraplantar Restrain the animal. Secure the hind limb. Insert needle at 30-degree angle carefully into the subcutaneous space of the plantar. Inject full volume of substance. The plantar region will become swollen.
  10. 10. Intramuscular injection Intravenous injection Intradermal injection
  11. 11. DAY 2 BLOOD GROUPING & BLOOD GLUCOSE MEASUREMENT  The ABO blood group system is the most important blood type system (or blood group system) in human blood transfusion.
  12. 12. • Determination of blood group is important in blood transfusion. WHY???? • The Rh (Rhesus) blood group system is one of the human blood group systems.
  13. 13. Measurement of blood glucose • Measure blood glucose level in blood • Method used : fasting blood sugar • Diseases : Diabetes mellitus -Type 1 and 2, Gestational Diabetes
  14. 14. Day 3 : Vaginal Swab
  15. 15. Introduction • Animal used : Rat • A vaginal swab test involves taking a sample of vaginal secretions with a device that looks like a cotton bud.
  16. 16. Proestrus Estrus Metestrus Diestrus/ pregnancy
  17. 17. Female Reproductive System • Reproductive system consists of two cervix (in rats) and only one (in mouse), a vagina, a uterine body, two uterine horns and a left and right ovary
  18. 18. Male Reproductive System • Male has a penis, a pair of accessory glands (seminal vesicles, bulbo-urethral glands and prostate glands) and a pair of testes
  19. 19. Day 4 Breathing & ECG
  20. 20. ELECTROCARDIOGRAM An electrocardiogram (ECG) is a test that checks for problems with the electrical activity of your heart. An ECG translates the heart's electrical activity into line tracings on paper. The spikes and dips in the line tracings are called waves.
  21. 21. An electrocardiogram (EKG or ECG) is done to: • Check the heart's electrical activity. • Find the cause of unexplained chest pain, which could be caused by a heart attack, inflammation of the sac surrounding the heart (pericarditis), or angina. • Find the cause of symptoms of heart disease, such as shortness of breath, dizziness, fainting, or rapid, irregular heartbeats (palpitations).