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In celebration of the International Biodiversity Day The Jordanian Society for Microbial Biodiversity (JMB) has held an interactive lecture in Wild Jordan\RSCN titled : the Importance of Microbial Biodiversity presented by Nura A. Abboud \the president of JMB

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  1. 1. ‫بسم ال الرحمن الرحيم‬ The Importance of Microbial Biodiversity Nura A. Abboud M.Sc Desert Studies Molecular MicrobiologyThe Jordanian Society for Microbial Biodiversity Biodiversity International Day RSCN May 22th 2012
  2. 2. The Jordanian Society for Microbial Biodiversity 2006 Back to Green JordanReports (microbiology, water management (,environmental policy 2011217
  3. 3. http://home.fnal.gov/~carrigan/pillars/Tree_of_life.htm
  4. 4. : The Prokaryote • First Earth inhibitor • Anaerobic • Harsh conditions • Photosynthesis • New ecosystem
  5. 5. 50 - 90% of all biomass on Earth is microbialhttp://home.fnal.gov/~carrigan/pillars/Tree_of_life.htm
  6. 6.  Everywhere!Harmful
  7. 7. Benign /beneficial...Necessary for all ecosystems
  8. 8. •Soil is adiversehabitat:Bacteria, fungi,archaea,protozoa& algae.
  9. 9. Importance of soil microbesoBiological Engine of the Eartho Maintaining soil functionsNutrient cycles: Nitrogen (N),Carbon (C),Phosphorus (P), Sulfur (S)
  10. 10. http://en.wikipedia.org/wiki/Nitrogen_fixation
  11. 11. X
  12. 12. Agricultural Applications ‫أهمية مايكروبات التربة‬
  13. 13. Pharmaceutical ApplicationsAntibioticsActinomycetes Streptomycin
  14. 14. Bacterocins•Bacteria & Archaea•Water purification•Food preservations•Veterinary Medicine•Agricultural Applications•Health Applications : Human ,plants & Animals
  15. 15. Medical LaboratoriesLab anddiagnosticsreagents
  16. 16. Industrial Applications Industrial bacterial enzymes :Lipase , amylase Instead of synthetic chemicals. Ex: to enhance detergents
  17. 17. Food industries:  Whole cells  Amino Acids  Vitamins
  18. 18. Biogas and Energy production + =
  19. 19.  Viruses generates electricityLuminescence bacteria lighting yourhouse Bacterial DNA
  20. 20.  Bacteria & Glue Medical ,dental & Engineering applications
  21. 21. Clostridium sporogenes Vs cancer
  22. 22. Biodiversity Greatest Earth treasures Microorganisms: most diverse & abundant 1-5% Huge Impact on our life
  23. 23. Research lab DNA based methods for characterizing microbial :communities 16r DNA Gene library Bacterial DNA Pl c t as or ve m id D NA
  24. 24. PCR : 16S rRNA-geneamplification TRFLP
  25. 25. 2 3 4 5 6 7 1
  26. 26. Halobacterium salinarum
  27. 27. ?What do HS do:AstrobiologyRepresentatives oflife in the universe ?
  28. 28. ? What can HS do
  29. 29. ?What do HS do Biological treatment tool :highly saline industrial waste effluents (Dong-Jin Ha et al – 2006(
  30. 30. ?What do HS do• Photobioreactor:• HS + solar = energy (H gas)• Daily Human consumption• (Zabut et al 2006)
  31. 31. CBD 1993
  32. 32. My Hypothesis: Jordan has highly diversecommunity of microorganisms
  33. 33. My recommendations:• Full survey : microbial biodiversityin Jordan• Genetic bank• Microorganisms & overgrazing …etc
  34. 34. Long term To gain a better understanding of the ecology of important microorganisms in the environmentQuestions we ask•Which bacteria are presentin a soil sample?•How many different types?•Which bacteria are activeand growing?•What is the role of bacteriain the context of theirenvironment?•How can we apply this
  35. 35. The onlybarrier is ourfaith in what we have
  36. 36. ! Thank youQuestions ?
  37. 37. References1) L.Prescott, J. Harely, D.Klein, Microbiology, fifth edition, McGraw-Hill Higher Education2) Mar. 2007 www.webdomino1.oecd.org/comnet/agr/soil_ero_bio.nsf/viewHtml/index/$FILE/Winding.PDF>3)Apr2007<http://webdomino1.oecd.org/comnet/agr/soil_ero_bio.nsf/viewHtml/index/$FILE/Winding.PDF >.4) Www.fuhrmanlab.usc.edu." Fuhrman Lab. University of southern California. Mar. 2007.5) Mary Anderson Home Page. Department of microbial ecology research group. 10 Apr. 2007<http://www.ucd.ie/indmicro/mary/technique.html>.6) Crecchio, C, A Gelsomino, R Ambrosoli, J Minati, and P Ruggiero. "Soil Biology &Biochemistry." Elsevier.2004 <www.elsevier.com>.7) Jones, Cm, and Je. Thies. "Soil Microbial Community Analysis Using Two-Dimensional Polyacrylamide GelElectrophoresis of the Bacterial Ribosomal Internal Transcribed Spacer Regions." Pubmed. 29 Apr. 2007<http://www.ncbi.nlm.nih.gov >.8) Bohme, Livia, and Frank Bohme. European Journal of Soil. 2006 <www.sciencedirect.com>.9) Paul, E., and F. Clark. Soil Microbiology and Biochemistry. 2nd ed. Academic P.10) "RFLP." U.S Food & Drug Administration. 9 Apr. 2007 <www.cfsan.fda.gov>.11) Campbell, N., and J. Reece. Biology. 6th ed. Benjamin Cummings.12) Polymerase Chain Reaction." Wikipedia. 6 Dec. 2005 <www.wikipedia.org>.13) Shivaji S. Microbial biodiversity: Shotgun libraries to metagenome. Indian J Med Microbial 2001; 19:171-17114) What is Microbiology.” Department of Molecular Biosciences-the University of Kansas. Mar. 2007<http://www2.ku.edu>.15) 16S RRNA: the Diagnostic Gene." Mar. 2007 <http://pilbara.mq.edu.au/wiki/16S_rRNA>.16) Experiment 9: Microbial Biodiversity." Mar. 2007www.plantpath.wisc.edu/fac/joh/Exp9DiversityStudentGuide.htm>.17) Mar. 2007 <www2.for.nau.edu/courses/pzf/Aronson_RestEco1993.pdf>.
  38. 38. 18) "Microbiology and Molecular Biology Reviews." Mar. 2007 <http://mmbr.highwire.org/>.19) Biodiversity of Soil Microbial Communities in Agricultural Systems." Mar. 2007 <http://www.springerlink.com>.20) Mar. 2007 <http://www.hcs.harvard.edu/~hsr/pdfswinter2003/cook26- 28.pdf>.21) Mar. 2007 http://www.cook.rutgers.edu/~dbm/forneypitfalls.pdf22) Mar. 2007 http://www.blackwell-synergy.com/doi/abs/10.1111/j.1574-6941.2006.00134.x23) 21 Mar. 2007 www.Montana.Edu.24) Jensen, D.b., M. Torn, and J. Harte. "In Our Own Hands: a Strategy for Conserving Biological Diversity inCalifornia," 1990:" 22 Mar. 2007 <www.ceres.ca.gov>.25) Mar. 2007 <http://www.unu.edu/env/plec/cbd/Montreal/papers/Scherr.pdf>.26) Mar. 2007 <www.microbes.org/biodiversity.asp>.27) Microb Zoo." Mar. 2007 <http://commtechlab.msu.edu/sites/dlc-me/zoo/>.28) SPECIAL SECTION: MICROBIAL DIVERSITY." Department of Microbiology, Maharshi Dayanand SaraswatiUniversity, Ajmer 305 009, India. Mar. 2007 <http://www.iisc.ernet.in/~currsci/jul102005/91.pdf>.29) Tree of Life Project." Mar. 2007 <http://tolweb.org/tree/phylogeny.html>.30) Mark Osborn, A., Edward R. B. Moore, and Kenneth N. Timmis. "An Evaluation of Terminal-RestrictionFragment Length Polymorphism (T-RFLP) Analysis for the Study of Microbial Community Structure andDynamics." Mar. 2007 <www.blackwell-synergy.com>.31) SPECIAL: BIODIVERSITY FOR FOOD AND AGRICULTURE." Mar. 2007 <www.fao.org/>.32) What is an Aridic Soil?" Soil Arid Regions of the United States and Israel. Mar. 2007<http://ag.arizona.edu/OALS/soils/aridsoils.html>.33) The International Arid Lands Consortuim. Mar. 2007 <http://ag.arizona.edu/OALS/IALC/Home.html>.34) IUCN the World Conservation Union. Mar. 2007 <http://www.iucn.org/themes/pbia/wl/docs/biodiversity/sbstta4/dry.pdf>.
  39. 39. 35) Office of Science-Department of Energy. Mar. 2007 <www.eurekalert.org/features/doe/2001-06/danl-md061402.php>.36) Microbial Diversity: Lets Tell It How It Is." Mar. 2007 <http://www.ncbi.nlm.nih.gov/books/bv.fcgi?Rid=coffeebrk.chapter.622>.37) The Search for Microbial Biodiversity." The Woodrow Wilson Foundation Leadership Programme for Teachers. Mar. 2007<http://www.woodrow.org/teachers/bi/1999/projects/group8/sediment1.html>.38) E. Marquis, Robert. "Bacteria" Encarta-Msn. 30 Apr. 2007 <www.encarta.msn.com>.39) M. Awramik, Stanley, and Robert E. Riding. Microbial Sediments. google. 30 Apr. 2007 <www.google.jo>.40) "The Microbial Community of the Soil Ecosystem." university college cork. 30 Apr. 2007 <www.ucc.ie>.41) "TAE." IBI. 1 May 2007 <www.sheltonscientific.com>. 42) El-Hage Scialabba, Nadia. "ORGANIC AGRICULTURE AND SOIL BIODIVERSITY." 1 May 2007<www.fao.org/organicag/doc/soil_biodiversity.htm>. 43) "What the Heck is PCR?" 13 May 2007 <http://www.hepatitis-c.de/pcr.htm>. 44) "PCR (Polymerase Chain Reaction)." 13 May 2007 <http://lifesciences.asu.edu/resources/mamajis/pcr/pcr.html>. 45) "Protocol for PCR with Taq DNA Polymerase." 13 May 2007<http://www.fermentas.com/techinfo/pcr/dnaamplprotocol.htm>.46) http://biosci.usc.edu/courses/2002-fall/documents/bisc300-lab_Hi-Lo_DNA_Marker.jpgAgarose Gel Electrophoresis of DNA." 13 May 2007 ) "47>.<http://arbl.cvmbs.colostate.edu/hbooks/genetics/biotech/gels/agardna.html>.May 2007 <http://www.bionewsonline.com/g/what_is_pcr.htm) 48Microbial Diversity and Function in Soil." May 2007 ) " 49>. <http://cropandsoil.oregonstate.edu/classes/CSS645/notes/Overview/CurOpinMicro_02-0240.pdfMay 2007 <http://216.109.125.130/search/cache? P=soil+microbiology+ARDRA &ei=UTF-8&Fr=yfp-t- ) 50 >. 501&u=plato.acadiau.ca/isme/Symposium16/muyzer. PDF&w= soil+microbiology+ardra&d=OOk7gurnOqj8&icp=1&.intl=us/http://gizfactory.com/article/philips-develops-micro-organisms-based-bio---light-http://www.msnbc.msn.com/id/12389963/ns/technology_and_science-science/t/bacteria-make-worlds-strongesthttp://www.dailymail.co.uk/health/article-2033871/Soil-bacteria-clostridium-sporogenes-new-weapon-cancer-fight.html

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