Cm4 microscopy

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by : Ms. Crizelda Liwanag

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Cm4 microscopy

  1. 1. microscopy
  2. 2. One of the most widely used tools in biological sciences An instrument that produces an enlarged image of an object Magnification – the increase in an object’s apparent size Resolution – the power of a microscope to clearly show detail
  3. 3. Principle: The microscope objective first forms in the tube a magnified aerial image of the specimen illuminated below the lamp and the condenser.The image is then viewed magnified through the eyepiece. microscope
  4. 4. What is the standard length of the draw tube? 160 mm Which is the most important component of the microscope? Objective lenses If the image remains visible after the exchange of objectives, the lenses are said to be_____ parfocal
  5. 5. I. Light microscopes 1. Simple 2. Compound a. Brightfield b. Darkfield c. Phase contrast d. Ultraviolet e. Fluorescence f. Stereoscopic II. Electron microscopes 1. transmission 2. scanning
  6. 6. Type of microscopy appearance magnification use Brightfield Microbe stained 1000x Overall appearance of most microbes Dark field Light microbe not stained 1000x Overall appearance of most microbes Phase contrast Cellular component s resolved 1000x Resolution of many cellular components
  7. 7. Type of microscopy appearance magnification use Ultraviolet UV absorbing portion of cell emitting light 1000x to 3000x Cellular components that absorb light Fluorescent Microbe fluorescing 1000x to 3000x m-o’s coated w/ dye fluoresce when struck by UV
  8. 8. Type of microscopy Appearance magnification use TEM Ultrathin section showing cellular component s (2-D) Up to 200,000x Study of viruses and detailed structure of larger m-o’s SEM Surface of microbe (3-D) Up to 50,000x Study of surface structure of m-o’s
  9. 9. Brightfield Microscope Dark image against a bright or white background
  10. 10. Thin, flat components of low refractile elements and living cells
  11. 11. polarized light for birefringent substances
  12. 12. Brightly illuminated specimen against a dark background
  13. 13. Fluorescent substances Filters: excitation & barrier
  14. 14. Electron beams & magnetic lenses

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