Citizen micro fierer_1-2012

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Citizen micro fierer_1-2012

  1. 1. Microbial  Life  in  Your  Home    Project  Directors:    Noah  Fierer  (Assoc.  Professor,  Univ.  of  Colorado)    Rob  Dunn  (Assoc.  Professor,  NC  State  Univ.)      Project  Personnel:    Holly  Menninger  (Director  of  Public  Science,              NC  State  Univ.)    Jon  Leff  (Research  Assoc.,  Univ.  of  Colorado)    Gilberto  Flores  (Postdoc,  Univ.  of  Colorado)  
  2. 2. Project  Goals:    1.  Explore  broad  paKerns  governing  the  distribuLon  of   bacteria  and  fungi  in  houses  across  the  U.S.  2.  Generate  interest  in  the  unexplored  microbial  diversity   found  all  around  us  3.  Engage  ‘ciLzen  scienLsts’  in  the  scienLfic  process  of   exploring  microbial  ecology  
  3. 3. Skin-­‐assoc.  taxa   Fecal-­‐assoc.  taxa   Soil-­‐assoc.  taxa  Flores  et  al.  PLoS  One.  2011.  
  4. 4. Microbial  biogeography  of  the  kitchen  PCo1  scores  –  kitchen  #1  
  5. 5. Propionibacterium  (#69)   Corynebacterium  (#6)  Pseudomonas  (#3)   Erwinia  (#12)  
  6. 6. Salmonella  (#409)  Campylobacter  (#302)   Listeria  (#1044)  
  7. 7. How  does  microbial  diversity  outside  homes  relate  to  that  found  inside  homes?      How  does  x  influence  house-­‐associated  bacterial  and  fungal  communiHes  across  the  U.S.?     x  =        presence  of  pets  in  the  home    climate    surrounding  land-­‐use  type  (e.g.  urban  vs.  rural)    house  occupancy    vegetarian/non-­‐vegetarian    house  type  (e.g.  free-­‐standing  vs.  apartment)    house  characterisLcs  (e.g.  carpeted  vs.  bare  floors)                
  8. 8. hKp://www.yourwildlife.org/  
  9. 9. Ongoing  work:     -­‐    Sequencing  and  analysis  of  samples  collected  from  20  homes  in   North  Carolina     -­‐  Environmental  scanning  electron  microscopy  of  key  house-­‐ associated  microbial  habitats   -­‐  Volunteers  recruited  from  >6,000  houses  across  the  U.S.  Planned  work  (next  6  months):     -­‐  Analyze  samples  from  >1000  houses   -­‐  IdenLfy  and  establish  “super-­‐sampler”  homes  for  longer-­‐term   sampling   -­‐  Design  experiments  and  more  directed  studies  to  build  on   research  findings  
  10. 10. Pilot  Study  (20  homes  near  Raleigh,  NC)  
  11. 11. Toilet  Seat   Pillow  case   TV  Screen   Refrigerator  shelf   Door  knob   Kitchen  counter   CuWng  Board  Door  Sill  (outer)   Door  Sill  (inner)  
  12. 12.    6,127  households  signed  up  to  date     (as  of  12-­‐28-­‐2011)      

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