Workshop Summaries


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Workshop Summaries

  1. 1. Nicolle A. Rosa MercadoWorkshop SummariesUPR CayeyBiol.4997Prof. E. Diaz and Prof. E. Gonzalez1) Importance and Pipetting PracticeIn this laboratory we reviewed pipetting concepts. We did large and small volume exercises with afocus on micro-pipets. Concepts, such as the uses of the first and second stops, were discussed. It isvery important to keep these concepts fresh due to the fact that they are the most practicedlaboratory techniques. Pipetting is the key to any experiment because, if the amount measured is notprecise or if the pipet is contaminated with another substance, everything might go wrong. Theexercises were verified based on the color obtained at the end. The amount was verified by (settingthe micro-pipettes to the total amount of substances that were placed in the tube to ensure that theamounts were measures accurately.2) Microscopy and PhotomicrographyDuring this laboratory, microscopy and micro-techniques were reviewed. It is of uttermostimportance for every scientist to know how to work with a microscope. Microscopes provide greatevidence of discoveries that have been made. They are also very helpful for studying differentmicroscopic organisms, such as bacteria. Here we reviewed the uses of different parts of themicroscope. We also learned how to take pictures using QCapture. The class was divided into fourgroups and each learned a different micro-technique. Each individual was assigned to teach anotherstudent how to use the technique he had learned. My task consisted of learning how to use afluorescence microscope to teach the technique to one of my colleagues. I was taught how to use thephase-contrast microscope. In conclusion, each student learned how to work with two differentmicro-techniques and reviewed the uses of the microscope’s parts.3) Workshop UNC - From DNA to ProteinIn this three day laboratory experience we were taught several genomics and proteomics techniques.We reviewed techniques such as agarose gel electrophoresis and SDS-PAGE. The first day of theworkshop we were taught how to extract our own DNA using easy-to-find products. During thesecond day, we ran a PCR and read the results through agarose gel electrophoresis. Therepresentatives of the University of North Carolina at Chapel Hill also helped us review the centraldogma of biology and how these procedures work. The third day we spoke about proteins and theirimportance in lysosomal storage disorders. We discussed how to prepare SDS-PAGE, WesternBlots, and Coomassie stains. These techniques are very common laboratory tools. They are veryimportant in biology and, specifically, in genetics because they help scientists to differentiate genesand proteins due to characteristics, such as their size, that can only be observed by using these tools.A person who knows how to do these techniques has a great advantage in the scientific community.