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Types of cell culture

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This slide gives an outline about the types of cell culture

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Types of cell culture

  1. 1. TYPES CELL CULTURE PRESENTED By: Nadia
  2. 2. TYPES OF CELL CULTURE Cell culture is classified into three: i. Primary cell culture • Adherent cell culture • Suspension cell culture ii. Secondary cell culture iii. Cell line • Finite cell line • Continuous cell line
  3. 3. TYPES OF CELL CULTURE Cell culture is classified into three: i. Primary cell culture • Adherent cell culture • Suspension cell culture ii. Secondary cell culture iii. Cell line • Finite cell line • Continuous cell line
  4. 4. PRIMARY CELL CULTURE Separate cell directly from the parent tissue ( kidney, liver, heart etc.) Separated by enzymatic or mechanical method. Maintaining the growth of the cell in a culture medium using glass or plastic container. Culture medium o Liquid or gel designed to maintain the growth of the cell. o It varies from different type of cell.
  5. 5. PRIMARY CELL CULTURE Primary cell culture is further classified based on the type of cell: i. Adherent cell : • Those cell which attach to the surface of the culture flask. • Forms a monolayer. • They have to be detached from the surface before they get sub cultured. • Growth limited to surface area. ii. Suspension cell • Those cell does not get attached to the surface of culture flask. • They are free floating. • Cells in blood stream. • Growth is limited to concentration of cells.
  6. 6. SECONDARY CELL CULTURE When primary cell is sub cultured then it is secondary cell. Sub culture;  Transfer of cell from one culture vessel to another.  Method: o Remove growth medium. o Detach adherent cells (enzymatic method)  Why required: o To provide fresh nutrients. o To provide more space.
  7. 7. CELL LINE Finite cell lines  Limited life span  Go through a limited number of cell generation  Properties: o Contact inhibition o Density limitation o Anchorage dependence  Less growth rate  Doubling time (24-92hrs.) Continuous cell lines Grow indefinitely Grows either in monolayer or in suspension Properties: o Absence Contact inhibition o Absence Anchorage dependence High growth rate Doubling time (12-24hrs.)

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