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An RNA reset button

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An RNA reset button

  1. 1. An RNA Reset Button Bumgarner and colleagues Molecular Cell 2012 Presented by Nacho CaballeroFriday, September 7, 12
  2. 2. Friday, September 7, 12
  3. 3. Flo11 is a yeast cell surface glycoprotein required to form invasive filaments Flo11Friday, September 7, 12
  4. 4. Flo11 is a yeast cell surface glycoprotein required to form invasive filaments Flo11 Flo11 shows variegated expressionFriday, September 7, 12
  5. 5. FLO11Friday, September 7, 12
  6. 6. Long non-coding RNAs regulate the variegated transcription of Flo11 ICR1 FLO11 PWR1Friday, September 7, 12
  7. 7. Long non-coding RNAs regulate the variegated transcription of Flo11 Flo11 ICR1 FLO11 PWR1Friday, September 7, 12
  8. 8. Long non-coding RNAs regulate the variegated transcription of Flo11 Flo11 ICR1 FLO11 PWR1Friday, September 7, 12
  9. 9. Long non-coding RNAs regulate the variegated transcription of Flo11 Flo11 ICR1 FLO11 PWR1 But who regulates them?Friday, September 7, 12
  10. 10. ICR1 FLO11 PWR1Friday, September 7, 12
  11. 11. ICR1 FLO11 Rpd3L Remodeler PWR1Friday, September 7, 12
  12. 12. Transcription factor Flo8 activates Flo11 expression ICR1 FLO11 Flo8 Rpd3L Remodeler PWR1 Sfl1Friday, September 7, 12
  13. 13. Transcription factor Flo8 activates Flo11 expression ICR1 FLO11 Flo8 Rpd3L Remodeler PWR1 Sfl1Friday, September 7, 12
  14. 14. Transcription factor Flo8 activates Flo11 expression Flo11 ICR1 FLO11 Flo8 Rpd3L Remodeler PWR1 Sfl1Friday, September 7, 12
  15. 15. ICR1 FLO11 PWR1Friday, September 7, 12
  16. 16. Transcription factor Sfl1 silences Flo11 expression ICR1 FLO11 Sfl1 PWR1Friday, September 7, 12
  17. 17. Transcription factor Sfl1 silences Flo11 expression ICR1 FLO11 Sfl1 PWR1 Flo8Friday, September 7, 12
  18. 18. Transcription factor Sfl1 silences Flo11 expression ICR1 FLO11 Sfl1 Hda1 Remodeler PWR1 Flo8Friday, September 7, 12
  19. 19. Transcription factor Sfl1 silences Flo11 expression Flo11 ICR1 FLO11 Sfl1 Hda1 Remodeler PWR1 Flo8Friday, September 7, 12
  20. 20. ICR1 FLO11 PWR1Friday, September 7, 12
  21. 21. Infrequent transcription of ICR1 resets the system ICR1 FLO11 PWR1 Flo8 Sfl1Friday, September 7, 12
  22. 22. Infrequent transcription of ICR1 resets the system ICR1 FLO11 PWR1 NowFlo8 and Sfl1 can compete for binding Flo8 Sfl1Friday, September 7, 12
  23. 23. FLO11 ON PWR1 ICR1 Flo8 Sfl1Friday, September 7, 12
  24. 24. FLO11 OFF PWR1 ICR1 Flo8 Sfl1Friday, September 7, 12
  25. 25. FLO11 PWR1 ICR1 RESET Flo8 Sfl1Friday, September 7, 12
  26. 26. Friday, September 7, 12
  27. 27. Rpd3L Chromatin remodeling Hda1 Remodeler Remodeler makes each state heritableFriday, September 7, 12
  28. 28. Rpd3L Chromatin remodeling Hda1 Remodeler Remodeler makes each state heritable The act of transcription ICR1 is functional, not the transcriptFriday, September 7, 12
  29. 29. Rpd3L Chromatin remodeling Hda1 Remodeler Remodeler makes each state heritable The act of transcription ICR1 is functional, not the transcript Most information in the non-coding genome remains hiddenFriday, September 7, 12

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