Onion and cheek cell, DNA extraction lab

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Onion and cheek cell, DNA extraction lab

  1. 1. Name _______________________________ Date ____________________ Per. _____ Observing Cells LabInstructions: Create a lab report beginning on page 70 of your INB. The title of this lab isObserving Cells Lab. After reading through the lab procedures, write a thoroughintroduction explaining the purpose of this lab. Use your knowledge about plant andanimal cells to add details to the introduction. Write the materials and procedures forpart A under your introduction. Create an appropriate data table under the proceduresfor part A. Next, write the materials and procedures for part B and create anappropriate data table. For part C, go to the websites listed. Complete the virtual DNAextraction lab first. Then read the procedures for How to Extract DNA from Any LivingThing. Write procedures for extracting DNA from split peas. After extraction, you will belooking at the DNA under the microscope, so add that as a step in your procedures andcreate an appropriate data table for the lab.Part A) ONION CELL LABMaterials:OnionIodine stainSlide and cover slipDropperMethod:1. Obtain a thin slice of onion from the teacher.2. Put a drop of iodine stain on a slide. - Be careful, stains can ruin clothing and other materials.3. Place onion slice in iodine stain on the slide. The slice should not be wrinkled orfolded.4. Put a cover slip over the stain.5. Observe under low, medium and high powers.6. Draw your observations on your paper for low, medium, and high powers. a. Label the cell membrane, nucleus, cytoplasm, and any other organelles youobserve (i.e. vacuoles).7. Place your used materials in the designated area.Part B) CHEEK CELL LABMaterials:ToothpickIodine stainSlides and cover slipsDropper
  2. 2. Name _______________________________ Date ____________________ Per. _____ Observing Cells LabMethod:1. Obtain a clean slide and cover slip.2. Put a drop of iodine stain on a slide. - Be careful, stains can ruin clothing and other materials.3. Gently rub the end of a toothpick on the inside of your cheek. - Be careful! Do not gouge out your cheek.4. Stir the toothpick in the iodine stain.5. Immediately, throw the toothpick away.6. Put a cover slip over the stain.7. Observe under low, medium and high powers.8. Draw your observations on your paper for low, medium, and high powers. a. Label the cell membrane, nucleus, cytoplasm, and any other organelles youobserve (i.e. vacuoles).9. Place your used materials in the designated area.Part C) DNA EXTRACTION LABVirtual DNA Extraction lab –http://learn.genetics.utah.edu/content/labs/extraction/How to Extract DNA from Any Living Thing -http://learn.genetics.utah.edu/content/labs/extraction/howto/

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