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Mycobacterium.ppt

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Mycobacterium.ppt

  1. 1. DIAGONOSIS OF MYCOBACTERIUMTUBERCULOSISBYMUHAMMAD ZADA
  2. 2. Introduction Pathogenic bacteria of mycobacteraceae family Aerobic bacilli –non spore formingnon motile Cell wall –rich in lipids Acid-fast bacilli Very slow growing Route of transmission- aerosole
  3. 3.  TB drug resistance: resistant to one or more of firstline drugs: isoniazid, rifampin, pyrazinamide andethambutol (~ 5% in Canada) MDR-TB: resistance to at least INH and rifampin (~0.7% in Canada) XDR-TB: resistance to all first line therapeutic drugs(rare)
  4. 4. Diagnostic Testing Culture is still the recommended method fordiagnosing TB Detection of AFB in respiratory secretions Chest X-ray Tuberculin skin test (TST) Molecular diagnostic tests
  5. 5. procedure Pulmonary or extra pulmonary samples are firstdecontaminated (NAL soln.) Smear formation Inoculation to LJ and MGIT media.
  6. 6.  Smear formation
  7. 7.  After inoculation of specimen the MGIT are kept inbactec for 42 days If any tube appears positive, It should be sub cultured, acid fast stained andtreated as presumptive positive
  8. 8.  thank you

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