Chapter 8 liquid chromatograph

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Chapter 8 liquid chromatograph

  1. 1. Chapter 8 Liquid Chromatograph <ul><li>8.1 Introduction </li></ul>High pressures High speed High performance High sensitivity
  2. 2. 8.2 Principles Adsorption process Xm + n Sa Xa + n Sm (1)Liquid – Solid chromatography 1.Classification
  3. 3. (2) Liquid – Liquid Chromatography Partition Process Xm Xs
  4. 4. (3) Ion Exchange Chromatography X + + Y(resins) Y + + X(resins)
  5. 5. (4) Ion pair Chromatography X + a + Y - a X + Y - org
  6. 6. 2. Column efficiency of LLC Eddy diffusion Longitudinal diffusion
  7. 7. Mass transfer
  8. 9. H- U curve of GC and LC
  9. 10. 3. Separation of Components <ul><li>(1) Resolution R </li></ul>(2) Separation Factor 7-19 7-20
  10. 11. 4. Separation Equation of LC Assume: Y 1 =Y 2 =Y, k 1 ≈k 2 =k 7-21 7-22 8-23
  11. 12. 5. Factors affect Resolution of a column (1) Plat number n = f ( L,H ) H =f ( u,k,D m ,D s , ….) (3) Selectivity factor α = k 2 / k 1 (2) capacity factor k = p / q P,q relay to mobile & stationary phase
  12. 14. 8.3 Instrumentation of LLC 1. LLS System (1) Reservoirs (2) Pump (3) Sample Injection Port (4) Column (5) Detector
  13. 16. 2. stationary phase 3. mobile phase (solvent) Porous micro beads support Pellicular micro beads support Polarity order N-Heptane < … < water
  14. 17. 4. Gradient elution
  15. 18. 5. Detector

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