The Impact of Genome Technology in
        the Oil Palm Industry
                  A presentation by
                  Dr....
Presentation Flow

Global Challenges: Developing Sustainable Future

Application of biotechnology in the oil palm industry...
Developing Sustainable Future

                                              Securing role in
                            ...
The Application of Biotechnology
Industry Goals: 2020

                                                           “It is timely for
35 MT FFB / ha         ...
Productivity Improvement in DNA Synthesis and Sequencing

 Significant improvements in key technologies used to study and ...
Oil Palm Genome Assembly Statistics
• This is the world’s first project that only used read generated from so called “next...
Largest 454 Genome Sequencing Project in the World


                                                                     ...
Other Plant Genome Projects
 Genome             Size of
                                        Approach             Time ...
Value Creation Potential
Identification of genetic markers for oil palm traits improvement/control
Oil Palm Traits Improvement




                    High                                  High Iodine             Disease ...
End in Mind: Higher Productivity
                                                                              Stacking ex...
Conventional Breeding vs. Marker Assisted Breeding
           Conventional Breeding                                       ...
Breeding Technology Pipeline




                                                                                         ...
Revolutionizing a Bio-based Economy

    Seeds of Wealth                           Green Fingers                          ...
Re-cap
By 2020, the industry targets to achieve yields of 35 MT FFB/hectare and with 25% OER

Elements of biotechnology ar...
For more information on the oil palm genome:-

                             Dr. K. Harikrishna
                           ...
Thank you
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09 CeoMeeting- Session 3- Sime Darby

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Session 3: Agriculture & Nutrition

Title : Doubling Crop Productivity And Ensuring Nutritional Security

Dr. K. Harikrishna, Sime Darby

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09 CeoMeeting- Session 3- Sime Darby

  1. 1. The Impact of Genome Technology in the Oil Palm Industry A presentation by Dr. K. Harikrishna Head of Research, Quantum Leap R&D Sime Darby R&D Centre October 6, 2009 for CEO’s Life Sciences Conference hosted by Malaysian Life Science Capital Fund (MLSCF) and Malaysia Technology Development Corporation (MTDC) 1
  2. 2. Presentation Flow Global Challenges: Developing Sustainable Future Application of biotechnology in the oil palm industry Productivity improvement in DNA synthesis and sequencing Oil palm genome assemble statistics Oil palm traits improvement and value creation potential Conventional breeding vs. Marker assisted breeding Breeding technology pipeline End in mind: Trait stacking for higher productivity Revolutionizing a bio-based economy The Impact of Genome Technology in the Oil Palm Industry |© 2009 Sime Darby Research Sdn Bhd 2
  3. 3. Developing Sustainable Future Securing role in agriculture development Global Challenges Ensuring food Environmental Urbanization security conservation Food consumption Food production Land and water Nurturing communities Promoting innovation to solve global challenges – opportunities for R&D! The Impact of Genome Technology in the Oil Palm Industry |© 2009 Sime Darby Research Sdn Bhd 3
  4. 4. The Application of Biotechnology
  5. 5. Industry Goals: 2020 “It is timely for 35 MT FFB / ha Malaysia to make 25% OER a paradigm shift to boost oil palm yields via biotechnology.” Datuk Seri Najib Razak Prime Minister The Impact of Genome Technology in the Oil Palm Industry |© 2009 Sime Darby Research Sdn Bhd 5
  6. 6. Productivity Improvement in DNA Synthesis and Sequencing Significant improvements in key technologies used to study and manipulate biological systems at the molecular level—tools for sequencing and synthesizing DNA are opening the door to a new era of genome engineering and design. 1st Generation 2nd Generation 3rd Generation Sanger-Based Cyclic-Array Method Single Molecule Sequencing Sequencing Sequencing Information Capacity 100’s 10,000,000’s 1,000,000,000’s (reads per experiment) Scalability of Sample Hundreds at a time A few at a time Prep Easily automated No amplification Amplification Required True direct DNA measurement Analog Analog Digital Sequencing Accuracy Resolves copied DNA of Averages data from Single DNA molecule varying lengths amplified DNA copies resolution USD1,000,000’s USD100,000’s USD10,000’s to Cost per Human Genome USD1,000’s The Impact of Genome Technology in the Oil Palm Industry |© 2009 Sime Darby Research Sdn Bhd 6
  7. 7. Oil Palm Genome Assembly Statistics • This is the world’s first project that only used read generated from so called “next generation” sequencers as the data input for de novo assembly of the oil palm genome • Sime Darby has sequenced the oil palm genome (30 fold coverage – 60 billion base pairs) – the oil palm genome is approximately 1.7 billion base pairs in size • It is the largest genome in world history assembled using 100% latest-generation sequence reads as data input Over 60% Repeats Makes Assembly Very Challenging Estimated coverage • 93.8% Estimated size of genome • 1.7 billion base pairs Assembled genome size • 1.594 billion base pairs The Impact of Genome Technology in the Oil Palm Industry |© 2009 Sime Darby Research Sdn Bhd 7
  8. 8. Largest 454 Genome Sequencing Project in the World DNA Base Pairs 66,205,000,000 Number of contigs Reads 238,151,400 Coverage 93.8% Assembly stage The Impact of Genome Technology in the Oil Palm Industry |© 2009 Sime Darby Research Sdn Bhd 8
  9. 9. Other Plant Genome Projects Genome Size of Approach Time Taken Assembly Sequencing Genome Team Used (months) Coverage % Project (millions bp) 10 global Rice 464 Sanger 86 77% institutions 5 institutes in Sanger WGS, Papaya 372 38 China and 101% Sanger BACs Hawaii DOE + 33 Sanger WGS, Poplar 480 48 other global 98% Sanger BACs institutions 454 BACs and Cucumber 370 NA 2 companies 54% WGS Sime Darby’s 454 BACs and Sime Darby Oil Palm 1700 15 93.8% WGS and Synamatix genome The Impact of Genome Technology in the Oil Palm Industry |© 2009 Sime Darby Research Sdn Bhd 9
  10. 10. Value Creation Potential Identification of genetic markers for oil palm traits improvement/control
  11. 11. Oil Palm Traits Improvement High High Iodine Disease Height Oil yield Long stalk carotene Value resistance reduction Screening Oil Palm Populations for Desired traits The Impact of Genome Technology in the Oil Palm Industry |© 2009 Sime Darby Research Sdn Bhd 11
  12. 12. End in Mind: Higher Productivity Stacking exponentially increases value Oil yield Trait retail Iodine Value value added per hectare Height reduction 1 Double Triple Trait stacking or pyramiding stack stack 1 = Indexed value of single trait The Impact of Genome Technology in the Oil Palm Industry |© 2009 Sime Darby Research Sdn Bhd 12
  13. 13. Conventional Breeding vs. Marker Assisted Breeding Conventional Breeding Marker Assisted Breeding Steps Procedures Duration (years) Duration Steps Procedures (years) DxD / TxT&TxP 1 year fingerprints DxD / TxT Growing of trials in the ~10 years Selection of selected dura 5 years Selection of field dura/tenera & and dura/tenera pisifera tenera/pisifera and pisifera Progeny DxT/P trials of DxT, ~10 years fingerprints 6-7 years DxP & field confirmation Using results 2 years after Using results Seed 1 from DxD, completion Seed from DxP production maximum after TxT/P trials of trials production confirmation confirmation trials TOTAL ~22 years TOTAL 14 years The Impact of Genome Technology in the Oil Palm Industry |© 2009 Sime Darby Research Sdn Bhd 13
  14. 14. Breeding Technology Pipeline Currently, we are developing reagents for breeding and mapping of SSR’s and SNP’s. Source: A. R. Fernie and N. Schauer , Metabolomics-assisted breeding: A viable option for crop improvement? The Impact of Genome Technology in the Oil Palm Industry |© 2009 Sime Darby Research Sdn Bhd 14
  15. 15. Revolutionizing a Bio-based Economy Seeds of Wealth Green Fingers Futuristic Diet • Raw materials • Premium agriculture • Breeding by design for food, land create novel crops biofuels and • Raw material produce • Offer balanced biomaterials will invigorate the nutritional value • Gate keeper of industry the economy • Preserving biodiversity Consumer Plant-made Connoisseurs Right Chemistry Pharmaceuticals • Choice of food with • Emergence of green • Tailored oil palm to good shelf life, chemical, address specific taste and texture biodegradable needs • Able to consume biopolymers & • Tackle major organic, cisgenics biofuels disease today and or conventional future palm oil The Impact of Genome Technology in the Oil Palm Industry |© 2009 Sime Darby Research Sdn Bhd 15
  16. 16. Re-cap By 2020, the industry targets to achieve yields of 35 MT FFB/hectare and with 25% OER Elements of biotechnology are currently used in the oil palm industry such as biogas from POME, clonal production for elite palms and molecular marker Significant improvements in tools for sequencing and synthesizing DNA are opening the door to a new era of genome engineering and design Sime Darby has sequenced the oil palm genome (30 fold coverage – 60 billion base pairs) – the oil palm genome is approximately 1.7 billion base pairs in size with over 90% coverage Identification of genetic markers through genetic marker assisted selection & conventional crossing and recombinant DNA technology will contribute to oil palm traits improvement and control Trait stacking will exponentially increase the retail value added per hectare We are able to shift towards a green sustainable economy largely based on renewable resources or known as the “bio-based” economy The Impact of Genome Technology in the Oil Palm Industry |© 2009 Sime Darby Research Sdn Bhd 16
  17. 17. For more information on the oil palm genome:- Dr. K. Harikrishna Chief Executive Officer Sime Darby Genomics Sdn. Bhd. No. 2, Jalan Tandang, 46050 Petaling Jaya, Selangor. Phone : 03 - 7787 8900 Fax : 03 - 7781 0298 E-mail : collaboration@simedarbygenomics.com Web : http://plantation.simedarby.com/genomics The Impact of Genome Technology in the Oil Palm Industry |© 2009 Sime Darby Research Sdn Bhd 17
  18. 18. Thank you

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