Chromatography

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  • Mixtures = analyte
  • Adsorbent = atoms that accumulate on the surface of the material
  • Chromatography

    1. 1. CHROMATOGRAPHY Nur Eliya Jasmi Nur Amalina Mohd Noor Nur Hamizah Salim Nur Azirah Norzaini Tan
    2. 2. What is CHROMATOGRAPHY ? colour to write
    3. 3. WHAT IS CHROMATOGRAPHY ? <ul><li>A technique for separating mixtures into their components in order to analyze , identify , and purify the mixture or components. </li></ul>
    4. 4. DEFINITION <ul><li>Analyze : To examine the mixture or structure or something especially by separating it into its parts. </li></ul><ul><li>Purify : To make something pure by removing substances that are not wanted out of another substances that contains it. </li></ul><ul><li>Identify : To recognize something and to determine the identity of mixture. </li></ul>
    5. 5. CHROMATOGRAM <ul><li>The visual output of the chromatograph. Different patterns on the chromatogram correspond to different components of the separated mixture </li></ul>
    6. 6. CHROMATOGRAM x axis – Retention time y axis – Solute concentration that leaving a chromatographic column. Retention time : Time taken for the analyte to pass through the system under set conditions.
    7. 7. PHASE IN CHROMATOGRAPHY ?
    8. 8. STATIONARY PHASE ADSORBENT : atoms that accumulate on the surface of the material
    9. 9. TYPE OF STATIONARY PHASE
    10. 10. MOBILE PHASE ELUENT : solvent that carry the analyte in elution.
    11. 11. MOBILE PHASE SUPER CRITICAL FLUID: mobile phase is a fluid above and close to its critical temperature and pressure.
    12. 12. COMPARISON OF THE PHASE MOBILE PHASE GAS GAS CROMATOGRAPHY (GC) LIQUID LIQUID CHROMATOGRAPHY (LC) STATIONARY PHASE LIQIUD GAS-LIQUID CHROMATOGRAPHY (GLC) SOLID GAS-SOLID CHROMATOGRAPHY (GSC) LIQUID LIQUID-LIQUID CHROMATOGRAPHY (LLC) SOLID LIQUID-SOLID CHROMATOGRAPHY (LSC)
    13. 13. ANALOGY…
    14. 14. R F VALUE It is the distance travelled by the sample or analyte divided by distance travelled by the solvent front in chromatography.
    15. 15. EXAMPLE OF R F VALUE
    16. 16. MECHANISME OF CHROMATOGRAPHY
    17. 17. ADSORPTION <ul><li>Surface phenomenon where interaction takes place only on the surface of one substance. </li></ul>
    18. 18. ABSORPTION <> ADSORPTION
    19. 19. ABSORPTION <> ADSORPTION
    20. 20. ELUTION <ul><li>The process of extracting a substance that is adsorbed to another by washing it with a solvent.   </li></ul><ul><li>    </li></ul>
    21. 21. ELUTION
    22. 22. PARTITION S aq  <==> S org
    23. 23. PARTITION
    24. 24. SUMMARIZE OF CHROMATOGRAPHY
    25. 25. IF COLOURLESS ???
    26. 26. ULTRAVIOLET DETECTOR
    27. 27. ULTRAVIOLET DETECTOR MECHANISM
    28. 28. THE END

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