MasterPlex QT v4 Power User Tips (webinar)


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MasterPlex® QT v4.0 is our Multiplex Quantitative Analysis Curve-Fitting Software for the Luminex xMAP and Bio-Plex® Platforms

This PowerPoint presentation accompanies a recording of our webinar on Tips for MasterPlex QT v4 Power Users. To view the recording:

To download a FREE trial version:

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MasterPlex QT v4 Power User Tips (webinar)

  1. 1. Live Webinar: 10 Tips for MasterPlex QT v4.0 Power Users Robert Lynde and Allen Liu View Recorded Webinar at:
  2. 2. Overview 1) Automatically Detect Outliers 2) Marking Wells and Grouping of Replicates 3) Entering Standard Information 4) Best Fit and Best Fit Parameters 5) Plotting Unknown Data Points on the Standard Curve 6) WYSIWYG Data Table 7) Chart Customization 8) Templates 9) Custom Reports 10) Virtual Plate
  3. 3. Automatically Detect Outliers <ul><li>Mark and select thresholds based on: </li></ul><ul><ul><li>MFI </li></ul></ul><ul><ul><li>Concentration </li></ul></ul><ul><ul><li>Count </li></ul></ul><ul><ul><li>Standard Deviation </li></ul></ul><ul><li>Options are located under Plate Preferences->Plate tab </li></ul><ul><li>Bonus Tip – AssayChex Process Control Panel plug-in </li></ul>
  4. 4. Marking Wells & Grouping of Replicates <ul><li>There are 4 different well types: </li></ul><ul><ul><li>Background </li></ul></ul><ul><ul><li>Standard </li></ul></ul><ul><ul><ul><li>Auto Fill Feature – works really nicely for serially diluted standards </li></ul></ul></ul><ul><ul><ul><li>Manual Input – one can also type in the values </li></ul></ul></ul><ul><ul><li>Unknown </li></ul></ul><ul><ul><li>Control </li></ul></ul><ul><li>The following stats will be calculated based on your replicates (for both MFI and Concentration): </li></ul><ul><ul><li>Standard Deviation </li></ul></ul><ul><ul><li>% CV </li></ul></ul><ul><ul><li>Mean </li></ul></ul><ul><li>Bonus Tips </li></ul><ul><ul><li>Group replicates with the group stamping feature </li></ul></ul><ul><ul><li>Pasting sample names </li></ul></ul><ul><ul><li>Batch dilution factor for unknowns </li></ul></ul>
  5. 5. Best Fit <ul><li>Leave it to the software to do all the hard work for you with the Best Fit feature </li></ul><ul><li>Optimize based on your chosen parameter with the default being RMSE </li></ul><ul><li>Optimize the Best Fit based on: </li></ul><ul><ul><li>Root mean square error (RMSE) </li></ul></ul><ul><ul><li>R-square </li></ul></ul><ul><ul><li>Standard deviation of % Recovery </li></ul></ul><ul><li>Options are located under Plate Preferences->Plate tab </li></ul>
  6. 6. Plotting Unknown Data Points on the Standard Curve <ul><li>Get a quick visual overview of where your unknown data points fall on the standard curves </li></ul><ul><li>Useful for determining whether your standard range is sufficient </li></ul><ul><li>Useful for determining whether the unknown data points fall onto to the linear or dynamic range </li></ul><ul><li>Bonus Tip – Zoom in and out on standard curve chart </li></ul>
  7. 7. WYSIWYG Data Table <ul><li>Customize the Data Table by: </li></ul><ul><ul><li>Adding/Removing data columns </li></ul></ul><ul><ul><li>Filtering data </li></ul></ul><ul><ul><li>Sorting data </li></ul></ul><ul><li>And then simply export to many different output formats including Excel </li></ul>
  8. 8. Chart Customization <ul><li>Easily change and configure many chart properties such as: </li></ul><ul><ul><li>Legends </li></ul></ul><ul><ul><li>Axes </li></ul></ul><ul><ul><li>Labels </li></ul></ul><ul><ul><li>General Appearance </li></ul></ul><ul><li>Bonus Tip – Sample order on X axis </li></ul>
  9. 9. Templates <ul><li>Save all your work into templates and reload them in seconds </li></ul><ul><li>Templates are available for: </li></ul><ul><ul><li>Plate layout and standard information </li></ul></ul><ul><ul><li>Data Table </li></ul></ul><ul><ul><li>Charts </li></ul></ul>
  10. 10. Custom Reports <ul><li>The Data Table is extremely flexible but it is not the one-size-fits-all solution </li></ul><ul><li>This is where the Custom Report feature leaves off </li></ul><ul><li>Download sample report templates from our download site at: </li></ul><ul><li> </li></ul>
  11. 11. Virtual Plate <ul><li>Easily merge different plate files together with the Virtual Plate feature </li></ul><ul><li>Handy for: </li></ul><ul><ul><li>Using standards on one plate for another plate </li></ul></ul><ul><ul><li>Stitching together 2 incomplete files from the same run </li></ul></ul><ul><ul><li>When 96 wells just is not enough for your assay </li></ul></ul>
  12. 12. Resources <ul><li>http:// </li></ul><ul><ul><li>Live Chat </li></ul></ul><ul><ul><li>User Community </li></ul></ul><ul><ul><li>Knowledge Base </li></ul></ul><ul><ul><li>Product downloads </li></ul></ul><ul><ul><li>Flash movies </li></ul></ul><ul><ul><li>Tutorials & documentation </li></ul></ul><ul><ul><li>Online Store http:// </li></ul></ul><ul><li>Email </li></ul><ul><ul><li>[email_address] (sales related inquiries) </li></ul></ul><ul><ul><li>[email_address] (support related inquiries) </li></ul></ul><ul><li>Phone </li></ul><ul><ul><li>888-615-9600 </li></ul></ul>10/15/09