AssayCheX TM  Process Control Panel for Luminex/Bio-Plex Immunoassays: Harnessing the true power of  xMAP technology Prese...
Webinar Recording <ul><li>This presentation accompanies a  webinar recording  which is available at: https://www1.gotomeet...
<ul><li>AssayCheX TM  Process Control Panel: an introduction </li></ul><ul><li>The product configuration </li></ul><ul><li...
Introduction <ul><li>AssayCheX™ Process Control Panel  is for use with Luminex xMAP based immunoassays. </li></ul><ul><li>...
Sandwich Immunoassay <ul><ul><li>In Sandwich immunoassays the capture antibodies to analyte are immobilized on microsphere...
4-plex Process Control Panel <ul><li>The AssayCheX process control panel is designed to provide assurance of the validity ...
The Product Configuration <ul><li>Size: 0.5 mL/vial </li></ul><ul><li>Each vial designed for for 5 plates </li></ul><ul><l...
Functionality of Each Microsphere Bead Region Name Functionality 97 Instrument Control Monitors the Luminex analyzer repor...
Immunoassays With AssayCheX..
How to use ? <ul><li>It’s as easy to use as…. </li></ul>Perform Assay Acquire Data Analyze Data 1 Add AssayCheX Microspher...
How to use with your assay <ul><li>Add the AssayCheX process control panel microspheres to your assay’s 1X microsphere mix...
Analysis Algorithm <ul><li>This 4-plex panel utilizes two independent acceptance-metrics to ensure that each step of the i...
Plate Failure Data
Individual Well Failure
Using with current Assays.. No AssayCheX With AssayCheX
Average Percentage Recovery Percentage recovery #  of two controls for 11 different analytes # using average MFI values of...
Value provided…. <ul><li>Verify individual data points </li></ul><ul><li>Confirm assay performance </li></ul><ul><li>Troub...
Value provided…. <ul><li>The Three Rs of AssayCheX </li></ul><ul><li>Remove outliers.  </li></ul><ul><li>Reduce retests.  ...
Value provided…. <ul><li>By Using AssayCheX and MasterPlex:  A powerful combination. </li></ul><ul><li>Define and check fo...
Resources <ul><li>assaychex.com </li></ul><ul><ul><li>Detailed product information </li></ul></ul><ul><li>miraibio.com </l...
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AssayCheX Process Control Panel for Luminex/Bio-Plex Immunoassays

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This presentation accompanies the webinar which is available at:

https://www1.gotomeeting.com/register/855617145

The AssayCheX process control panel is designed to provide assurance of the validity of each sample’s results for immunoassays performed using Luminex xMAP technology.

The webinar covers the following topics:

1. AssayCheXTM Process Control Panel: an introduction
2. The product configuration
3. Functionality of each microsphere in process control panel
4. How to use AssayCheX along with current assays
5. Value provided by AssayCheX process control panel
6. Data analysis with MasterPlex® QT AssayCheX plug-in

For more information, please visit:
http://www.miraibio.com/reagents/assaychex-process-controls.html

Published in: Technology, Business
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AssayCheX Process Control Panel for Luminex/Bio-Plex Immunoassays

  1. 1. AssayCheX TM Process Control Panel for Luminex/Bio-Plex Immunoassays: Harnessing the true power of xMAP technology Presenters: Neeraja Venkateswaran, Ph.D. from Radix Biosolutions Robert Lynde from MiraiBio Group of Hitachi Software
  2. 2. Webinar Recording <ul><li>This presentation accompanies a webinar recording which is available at: https://www1.gotomeeting.com/register/855617145 </li></ul>
  3. 3. <ul><li>AssayCheX TM Process Control Panel: an introduction </li></ul><ul><li>The product configuration </li></ul><ul><li>Functionality of each microsphere in process control panel </li></ul><ul><li>How to use AssayCheX along with current assays </li></ul><ul><li>Value provided by AssayCheX process control panel </li></ul><ul><li>Data analysis with MasterPlex ® QT AssayCheX plug-in </li></ul>Overview
  4. 4. Introduction <ul><li>AssayCheX™ Process Control Panel is for use with Luminex xMAP based immunoassays. </li></ul><ul><li>Two main components of xMAP technology are: </li></ul><ul><li>Flow based analyzer </li></ul><ul><li>Fluorescent microspheres. </li></ul>
  5. 5. Sandwich Immunoassay <ul><ul><li>In Sandwich immunoassays the capture antibodies to analyte are immobilized on microspheres and biotinylated detection antibody is used in combination with streptavidin-phycoerythrin (SAPE) for detecting the specific analyte. </li></ul></ul>
  6. 6. 4-plex Process Control Panel <ul><li>The AssayCheX process control panel is designed to provide assurance of the validity of each sample’s results for immunoassays performed using Luminex xMAP technology. </li></ul><ul><li>It is a 4 Plex panel </li></ul>
  7. 7. The Product Configuration <ul><li>Size: 0.5 mL/vial </li></ul><ul><li>Each vial designed for for 5 plates </li></ul><ul><li>It is a 4 plex panel with regions 97, 98, 99, 100 </li></ul><ul><li>Available for non magnetic assays </li></ul><ul><li>Research use only </li></ul><ul><li>Supplied in brown vial to minimize exposure to light </li></ul><ul><li>Shipped cold. </li></ul>
  8. 8. Functionality of Each Microsphere Bead Region Name Functionality 97 Instrument Control Monitors the Luminex analyzer reporter fluorescence measurement 98 Fluorescent Reporter Control Monitors the addition of the fluorescent reporter streptavidin-phycoerythrin (SA-PE) reagent to the well 99 Biotin Detector Control Monitors the addition of the biotin-labeled detector antibody reagent to the well 100 Negative or Non-specific Control Monitors the non-specific binding (if any) due to the sample matrix
  9. 9. Immunoassays With AssayCheX..
  10. 10. How to use ? <ul><li>It’s as easy to use as…. </li></ul>Perform Assay Acquire Data Analyze Data 1 Add AssayCheX Microspheres 2 Add Regions To Template 3 Export to MasterPlex
  11. 11. How to use with your assay <ul><li>Add the AssayCheX process control panel microspheres to your assay’s 1X microsphere mixture immediately prior to adding the microspheres to the wells. Add one µL (1 µL) of AssayCheX microspheres to the 1X microsphere mixture for each well to be tested. </li></ul><ul><li>Perform assay procedure as per the assay kit protocol. </li></ul><ul><li>For data acquisition, select the AssayCheX microsphere regions (namely 97, 98, 99, 100) in addition to the kit-specific regions in an xMAP data acquisition/analysis software package. </li></ul><ul><li>Mix microspheres well before use by vortexing or pipetting up and down. </li></ul>
  12. 12. Analysis Algorithm <ul><li>This 4-plex panel utilizes two independent acceptance-metrics to ensure that each step of the immunoassay is performed correctly and that the instrumentation is operating properly at the time of data acquisition. </li></ul><ul><li>Threshold value: For catastrophic reagent or instrument failure </li></ul><ul><li>Acceptance range: Range of high and low acceptance values to accommodate normal assay variance </li></ul><ul><li>To confirm the validity of each sample result during data analysis, plate threshold values and acceptance criteria ranges must be determined. </li></ul>
  13. 13. Plate Failure Data
  14. 14. Individual Well Failure
  15. 15. Using with current Assays.. No AssayCheX With AssayCheX
  16. 16. Average Percentage Recovery Percentage recovery # of two controls for 11 different analytes # using average MFI values of triplicate samples Anal 01 Anal 02 Anal 03 Anal 04 Anal 05 Anal 06 Anal 07 Anal 08 Anal 09 Anal 10 Anal 11 No AssayCheX control 1 % Recovery 105.66 82.38 89.52 93.20 70.32 72.56 107.18 92.88 184.30 105.01 91.47 control 2 % Recovery 77.87 79.00 77.06 98.37 65.04 181.94 85.19 83.00 93.46 77.31 81.17 With AssayCheX control 1 % Recovery 86.66 80.55 96.80 91.24 82.59 104.75 81.60 87.18 75.07 85.07 88.89 control 2 % Recovery 105.49 104.34 109.30 105.39 83.20 93.01 103.88 102.75 101.52 101.24 105.02
  17. 17. Value provided…. <ul><li>Verify individual data points </li></ul><ul><li>Confirm assay performance </li></ul><ul><li>Troubleshoot with greater ease </li></ul><ul><li>Instantly flag potential failures </li></ul><ul><li>Reduce the need for replicates </li></ul><ul><li>http:// www.assaychex.com/results.php </li></ul>
  18. 18. Value provided…. <ul><li>The Three Rs of AssayCheX </li></ul><ul><li>Remove outliers. </li></ul><ul><li>Reduce retests. </li></ul><ul><li>Reduce replicates. </li></ul><ul><li>How? </li></ul>
  19. 19. Value provided…. <ul><li>By Using AssayCheX and MasterPlex: A powerful combination. </li></ul><ul><li>Define and check for thresholds for each AssayCheX microsphere region in every well. </li></ul><ul><li>Flag failed wells for review. </li></ul><ul><li>Automatically mark failed wells as outliers and exclude them from further calculations. </li></ul><ul><li>Display results in an easy-to-read view and data table. </li></ul><ul><li>Automatically generate a final report of results. </li></ul><ul><li>http:// www.assaychex.com/value.php </li></ul>
  20. 20. Resources <ul><li>assaychex.com </li></ul><ul><ul><li>Detailed product information </li></ul></ul><ul><li>miraibio.com </li></ul><ul><ul><li>Online Store or sales@miraibio.com </li></ul></ul><ul><ul><ul><li>AssayChex – 5 plates for $495 through the end of November </li></ul></ul></ul><ul><ul><li>FREE Trial download of the MasterPlex software </li></ul></ul><ul><ul><li>Blog , Live chat, flash movies, tutorials, user community etc. </li></ul></ul><ul><ul><li>[email_address] </li></ul></ul><ul><li>radixbiosolutions.com </li></ul><ul><ul><li>Custom assay development </li></ul></ul><ul><ul><li>Custom production </li></ul></ul><ul><ul><li>Custom reagent production </li></ul></ul><ul><ul><li>Modified microspheres </li></ul></ul><ul><ul><li>[email_address] </li></ul></ul>

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