Business focus3Agronomy, Science Sustainable plantation, social inclusionfor Oils Supply Programme Collection of accessions – Germoplasmbank FMA for productivity and plant health Jatropha detoxification process Fungi productionIndustry Jatropha planting to crude oil Planting, maintenance andharvesting Expelling, preprocessing andlogistics Crude oil salesBiovale Energia
To set up a mycorrhizal germplasm reservoir of genetic diversity ofmycorrhizal fungi of jatropha-curcas with high oil yielding for biodieselproduction and the elimination of the toxicity of the meal]waste foranimal feed Executing organization :BIOVALE ENERGIA – an emerging biodiesel company based in the MinasGerais in partnership with various stakeholders in the oil production chain Co-executing organizations:Federal University of Viçosa – DMB/BIOAGRO, ranked as the best universityof Minas Gerais State and 3rd in BrazilSECTES – Minas Gerais State Science and Technology SecretariatEPAMIG – EMPRESA DE PESQUISA DE AGROPECUÁRIA DE MINAS, nationalbench-mark in jatropha developmentCENTRO TECNOLÓGICO DE MINAS GERAIS - CETECR&D goals and stakeholders
Biofuel productionhas becomesubstantially moreefficient over the last25 years as Brazil andthe United Stateshave scaled up theirindustries.Such incrementalgains are likely tocontinue for years tocome.New Technologies, New GainsHowever, the greatest potential for biofuels lies in thedevelopment of new technologies that will significantly expandthe range of biomass feedstock, increase conversion efficiencies,and lower production costs.
Documentation, preservation and characterization of J. curcasand mycorrhizal fungi germplasmDevelopment of micropropagated plants inoculated of ROC (RootOrgan Culture) of arbuscular mycorrhizal (AM) fungi and their invitro mass inoculum productionSelection of jatropha genotypes with high oil yielding, envisagingthe biodiesel productionToxicity elimination of the waste for animal feedUtilization and reclamation of industry created wastelandsExpected resultsThe mycorrhizal technology offers biological means of assuring plant health in aneconomically profitable and ecologically friendly manner. The only known fungal systemcategorized as a biofertilizer, mycorrhizae provide plant roots with extended arms thathelp them tap soil nutrients that are otherwise beyond their reach. This means greateravailability of other, enriching soil, increasing health, and decreasing dependence onchemical fertilizers.
It exists a wide diversity within the AM fungi which form associationwith the roots of almost 80% plant species and enhance mineralnutrient acquisition and water uptake, as well as increase tolerancetowards different environmental stresses conditions.To conserve and exploit their diversity, BioVale Project contemplatesto build J. curcas and mycorrhizal fungi culture depository housesand maintain cultures from different agro-ecological zones, indifferent areas of Brazil.The expected result is to trap isolate a variety of J. curcas andpolysporal/monosporal cultures, setting up a J. curcas andmycorrhizal fungi germplasm collection of genetic diversity ofagriculturally and industrially species for oil production .Documentation, preservation and characterizationof J. curcas and mycorrhizal fungi germplasm
Jatropha has been identified as one the best plantalternative to offer clean fuel for achieving energysecurity. Jatropha seeds inoculated with in vitro-raised mycorrhiza exhibits early fruition andflowers from the 7th month onwards as against ayear with conventional clonal plantations and twoyears from seed raised plantations.The mycorrhized Jatropha also exhibited 20%–30%higher yields as compared to non-mycorrhizalplantations.The mycorrhized Jatropha will be widely testedcovering several agro-climatic regions in Brazil toprove their adaptation in diverse soils.More and earlier productivity
The ROC (root organ culture) system is the mostattractive and advanced cultivation methodology forAM (arbuscular mycorrhizal ) fungi: it uses root-inducing transfer-DNA-transformed roots of a hostplant to develop the symbiosis on a specific mediumin vitro which provides pure, viable, contamination-free inoculum using reduced space.BioVale R&D Project aims at gaining expertise inROC for in vitro culture and utilize mass productionof AM fungi under in vitro.Development of ROC of different AM fungi and theirmass inoculum production under in vitro
Although the facts and figures of potential role of mycorrhizalassociation in enhanced nutritional and water needs of plants inlaboratories, the major bottleneck for its widespread applicationto reach the end-users is its bulk production to cater the hugerequirement.A known fact that culturing mycorrhizal fungi in laboratoryconditions like other microbes was not possible due to its strictbiotrophic nature of proliferation in the presence of suitablehost has been the major reservation of its future contribution inagriculture.The mass inoculum technology envisages to exploit thegenetically modified host roots using the Agrobacteriumumrhizogenes carrying Ri T-DNA plasmid. The technology offers themass production of viable, healthy, genetically pure and highquality fungal propagules, without any pathogenic contaminationunder in vitro environment.Mass inoculum technology
One of the main challenges that mankind will be facing during this century willbe the necessity to drastically increase animal production levels andproductivity in order to fulfill the feeding demands of a growing worldpopulation.J. curcas is a multipurpose tree of significant economic importance becauseof its several industrial and medical uses. Jatropha grows throughout most ofthe tropics. It survives on poor stony soils and is resistant to drought,reaches a height from 3 m to 8 m and can be used to reclaim land. The seedweighs approximately from 0.53 -0.86 g and its kernel contains 22 -27 %protein and 57 -63 % lipid indicating good nutritional value.The oil from these seeds can serve as fuel for diese! engines, indicating itspotential as a renewable energy source . The potential impact is immense forcountries with no indigenous fossil fuel or for regions remote from a source ofsupply. Furthermore, use of biodiescl is friendly to the environment. Theseeds can be transported without deterioration and at low cost due to its highspecific mass.However, the seed and oil have been found to be toxic to. Hence, its use as afood or feed source is presently limited.
Biological detoxification is another environmental sound approachfor making J. curcas suitable for animal feeding. White-rot fungalbasidiomycetes are well known for their ability to degrade a widerange of xenobiotics, such as polycyclic aromatic hydrocarbons,polychlorinated biphenyls and synthetic dyes due to their enzymaticsystems [7, 8].Enzymatic complexes produced by white-rot fungi have also anenormous potential to be used in the treatment of fibrousfeedstuffs improving its nutritive value through the sequentialdegradation of certain refractory cell wall componentsIncresing aggregated value of the residue J. curcasThe oil expell
Treatment of J. curcas seed cake by solid-state incubation with white-rot fungi isable to promote the decrease of phorbol esters content to non-toxic levels,showing that this might be an alternative treatment with future potential. In fact,most of the physical and chemical treatments being proposed so far have notbeen able to definitively decrease the levels of these toxic compounds. Thedevelopment of a biological approach to treat J. curcas seed cake is anenvironmentally sound and a more economical process, which might beadvantageous since it can effectively decrease the level of toxic compounds.End result
de germoplasma pinhãomanso:-Banco de germoplasma:exvitro (no campo) e in vitro- Caracterização molecularMontou-se o banco de germoplasma de pinhão manso no campus deFlorestal e em Viçosa, num total de 49 acessos.Foi ajustado protocolo para desinfestação, estabelecimento e proliferaçãode pinhão manso in vitro. Este protocolo permitiu montar banco degermoplasma in vitro.Uso dos reguladores BAP e AIA e dos antioxidantes ácido cítrico e ácidoascórbico no meio de crescimento é eficaz na indução de brotos emultiplicação permitindo formar banco de germoplasma in vitro dopinhão mansoCaracterização molecular de 46 acessos, somente três foram divergentes.Por possuírem alto valor em distância genética, podem ser utilizadoscomo genitores de um híbrido no programa de melhoramento
O substrato PlantMax isolado ou misturado com areia constitui-se umsubstrato adequado para o desenvolvimento vegetativo das sementes eestacas;O banco de germoplasma do pinhão-manso foi desenvolvido com sucessotanto em Viçosa –MG quanto em Florestal-MG;Pinhão-manso apresenta potencial para ser utilizado na micropropagaçãotanto de sementes quanto de estacas.Produção de mudas depinhão manso
Diversidade de Fungosmicorrízicosarbusculares (FMAs) emplantios de pinhão mansoForam identificadas 19 espécies de FMAs na rizosfera de plantas depinhão manso.O gênero Glomus foi o mais abundante em número de espécies entre osFMAs na rizosfera de pinhão manso nas áreas estudadas.Plantas de pinhão-manso ocorrendo em regiões distantes, porém, emcondições edafoclimáticas semelhantes, possuem populações de FMAssemelhantes próximas às raízes.A utilização em conjunto das metodologias morfológica e molecularfornece informações mais completas a respeito da diversidade de FMAspresentes na rizosfera das plantas em campo.Plantas de pinhão manso apresentam elevada percentagem de colonizaçãomicorrízica e relativa abundância no número de esporos na rizosfera.
Pleurotus ostreatus tem alta capacidade de degrada éster de forbol, ácidofítico e compostos ligninocelulósicos;P. ostreatus apresenta alta produtividade e eficiência biológica deprodução de cogumelos em substratos à base da torta de pinhão manso;Os cogumelos produzidos na torta de pinhão manso apresentam teor deéster de forbol em níveis considerados não tóxicos, podendo ser indicadopara o consumo, mas testes biológicos devem ser efetuados paracomprovar a inexistência dos compostos tóxicos ou anti nutricionais.
Característicasnutricionais de resíduode pinhão manso tratadocom fungo da podridãobrancaO fungo da podridão branca aumentou os teores de nutrientes na torta depinhão manso. Apesar da torta de pinhão manso ser tóxica, a partirdessascaracterísticas nutricionais a mesma serve para serem adicionados comofonte de proteína, carboidrato e energia.Enzymatic complexes produced by white-rot fungi have also an enormouspotential to be used in the treatment of fibrous feedstuffs improving itsnutritive value through the sequential degradation of certain refractory cellwall components
Avaliação dodesempenho dos animaisalimentados com ração àbase de torta de pinhãomanso tratada -digestibilidade in vivoA torta destoxificada de pinhão manso pode ser utilizada com segurançaem até 20% da MS na dieta de caprinos em crescimento como alimentoAlternativoIn vitro digestible organic matter (DOM), metabolizable energy (ME) and rumendegradable nitrogen