SlideShare a Scribd company logo
Characterisation of Pseudomonadaceae populations in Tuber melanosporum
                                  wild truffieres

                           Vilanova, X1*.; Sanchez, M1.; Viñas, M2. Morcillo, M. 1


1*
  Micología Forestal & Aplicada S. L.
Address* Rambla Arnau de Vilanova , 6 local D 08800 Vilanova i la Geltrú (Barcelona-Spain).
E-mail* xaviervilanova@micofora.com
URL* www.micofora.com
2
  Giro Joint Research Unit IRTA-UPC, IRTA, Torre Marimon, E-08140-Caldes de Montbui (Barcelona-Spain).



Abstract

In the last decade several surveys have revealed an important role of Pseudomonas genus as a Mycorrhizal
Helper Bacteria (MHB). Moreover, the bioaugmentation of environmental isolated Pseudomonas strains to the
substrate at inoculation time in different plant species throughout mycorrhization assays have been related
on the occurrence of a greater number of mycorrhiza of Tuber genus.

In this work, a selective culture medium was designed to isolate bacteria belonging to Pseudomonaceae
family from soil samples taken from two different wild truffières. The amount of culturable
Pseudomonadaceae populations was monitored using the selective medium throughout one year in different
wild truffières. A total of eight samples per monitored truffières were taken every 40 days for the two
monitored truffières. In the same area, non-truffle producing evergreen oaks were used as negative controls
being sampled simultaneously to the monitored truffières. The results revealed both a low diversity of
colonial morphology and a similar abundance of culturable Pseudomonadaceae strains on the selective-
designed agar medium throughout the one-year survey in both monitored truffières (6.44 x 104 ± 3.54 x 104
       -1                                    -1
CFU · g and 5.71 x 104 ± 3.25 x 104 CFU · g respectively (± standard deviation of n=8). Non-producing trees
showed similar colonial morphologies to those found from the truffières, but less abundant (2.54 x 104 ±
2.54 x 104 CFU · g-1of soil (± standard deviation of n=8)).

Finally, thirty samples from both truffières and controls were characterized by means of 16SrRNA gene-based
PCR-DGGE (Polymerase Chain Reaction (PCR) with a subsequent Denaturing Gradient Gel Electrophoresis
(DGGE). We obtained a low diversity (1-4 DGGE bands) in all the studied samples (both truffières and
controls).

It is noteworthy that most of detected bands migrated mainly in two defined positions from the DGGE gel,
corresponding to a two different ribotypes belonging to Pseudomonas chlororaphis and Stenotrophomonas
spp. respectively.



KEYWORDS

Pseudomonas, MHB, Tuber melanosporum, wild truffieres.

More Related Content

What's hot

Dynamic of dysinfectant
Dynamic of dysinfectantDynamic of dysinfectant
Dynamic of dysinfectant
firdousfatima9
 
Detection of Stall Region during Testing of Compressor
Detection of Stall Region during Testing of CompressorDetection of Stall Region during Testing of Compressor
Detection of Stall Region during Testing of Compressor
IJMER
 
Paola Caballero and Anthony Hernandez Scientific Manuscript
Paola Caballero and Anthony Hernandez Scientific ManuscriptPaola Caballero and Anthony Hernandez Scientific Manuscript
Paola Caballero and Anthony Hernandez Scientific Manuscript
anthonyhr
 
Diversity of hymenopteran parasitoids (Hymenoptera: Chalcididae) associated w...
Diversity of hymenopteran parasitoids (Hymenoptera: Chalcididae) associated w...Diversity of hymenopteran parasitoids (Hymenoptera: Chalcididae) associated w...
Diversity of hymenopteran parasitoids (Hymenoptera: Chalcididae) associated w...
arboreo.net
 
4.monica 13.felix phages report corrected
4.monica 13.felix phages report corrected4.monica 13.felix phages report corrected
4.monica 13.felix phages report corrected
felixjvalles
 
Poster_TR_07.09.16_RM_V2_FINAL
Poster_TR_07.09.16_RM_V2_FINALPoster_TR_07.09.16_RM_V2_FINAL
Poster_TR_07.09.16_RM_V2_FINAL
Trancizeo Lipenga
 
CHARACTERISING FOOT-AND-MOUTH DISEASE VIRUS IN CLINICAL SAMPLES USING NANOPOR...
CHARACTERISING FOOT-AND-MOUTH DISEASE VIRUS IN CLINICAL SAMPLES USING NANOPOR...CHARACTERISING FOOT-AND-MOUTH DISEASE VIRUS IN CLINICAL SAMPLES USING NANOPOR...
CHARACTERISING FOOT-AND-MOUTH DISEASE VIRUS IN CLINICAL SAMPLES USING NANOPOR...
EuFMD
 
final report
final reportfinal report
final report
Audrey Negrelli
 
Development and anlysis of a 20K SNP array for potato (Solanum tuberosum) an ...
Development and anlysis of a 20K SNP array for potato (Solanum tuberosum) an ...Development and anlysis of a 20K SNP array for potato (Solanum tuberosum) an ...
Development and anlysis of a 20K SNP array for potato (Solanum tuberosum) an ...
Peter Vos
 
QTL Mapping of Cotton Verticillium Wilt Resistance
QTL Mapping of Cotton Verticillium Wilt ResistanceQTL Mapping of Cotton Verticillium Wilt Resistance
QTL Mapping of Cotton Verticillium Wilt Resistance
PARTNER, BADC, World Bank
 
1-s2.0-S0923250811001872-main
1-s2.0-S0923250811001872-main1-s2.0-S0923250811001872-main
1-s2.0-S0923250811001872-main
Carolina Ruivo Pereira
 
Victor Poster FINAL PDF
Victor Poster FINAL PDFVictor Poster FINAL PDF
Victor Poster FINAL PDF
Victor Quach
 
Molecular study of fasciola spp
Molecular study of fasciola sppMolecular study of fasciola spp
Molecular study of fasciola spp
Akhtaruzzaman Biplob
 
Poster tuber borchii research in portugal iwemm morocco
Poster tuber borchii research in portugal iwemm moroccoPoster tuber borchii research in portugal iwemm morocco
Poster tuber borchii research in portugal iwemm morocco
Micologia Forestal & Aplicada
 
Antimicrobial Activity of Actinomycetes from Soil Samples of Some 6(35)April ...
Antimicrobial Activity of Actinomycetes from Soil Samples of Some 6(35)April ...Antimicrobial Activity of Actinomycetes from Soil Samples of Some 6(35)April ...
Antimicrobial Activity of Actinomycetes from Soil Samples of Some 6(35)April ...Ravindragouda Patil
 
9 diversity of meloidogyne in martinique
9 diversity of meloidogyne in martinique9 diversity of meloidogyne in martinique
9 diversity of meloidogyne in martinique
Ana Karenina
 
Genetic Diversity of Sorghum (Sorghum bicolor L. Moench) from East and West H...
Genetic Diversity of Sorghum (Sorghum bicolor L. Moench) from East and West H...Genetic Diversity of Sorghum (Sorghum bicolor L. Moench) from East and West H...
Genetic Diversity of Sorghum (Sorghum bicolor L. Moench) from East and West H...
Premier Publishers
 
TESS PURC Poster
TESS PURC PosterTESS PURC Poster
TESS PURC Poster
Mary Johnson
 
DEVELOPMENT OF LINEAGE-SPECIFIC REAL-TIME RT-PCR FOR THE RECENT FMDV, ASIA/SE...
DEVELOPMENT OF LINEAGE-SPECIFIC REAL-TIME RT-PCR FOR THE RECENT FMDV, ASIA/SE...DEVELOPMENT OF LINEAGE-SPECIFIC REAL-TIME RT-PCR FOR THE RECENT FMDV, ASIA/SE...
DEVELOPMENT OF LINEAGE-SPECIFIC REAL-TIME RT-PCR FOR THE RECENT FMDV, ASIA/SE...
EuFMD
 
READY-TO-USE SOLID PHASE BLOCKING ELISA KITS FOR DETECTION OF SPECIFIC ANTIBO...
READY-TO-USE SOLID PHASE BLOCKING ELISA KITS FOR DETECTION OF SPECIFIC ANTIBO...READY-TO-USE SOLID PHASE BLOCKING ELISA KITS FOR DETECTION OF SPECIFIC ANTIBO...
READY-TO-USE SOLID PHASE BLOCKING ELISA KITS FOR DETECTION OF SPECIFIC ANTIBO...
EuFMD
 

What's hot (20)

Dynamic of dysinfectant
Dynamic of dysinfectantDynamic of dysinfectant
Dynamic of dysinfectant
 
Detection of Stall Region during Testing of Compressor
Detection of Stall Region during Testing of CompressorDetection of Stall Region during Testing of Compressor
Detection of Stall Region during Testing of Compressor
 
Paola Caballero and Anthony Hernandez Scientific Manuscript
Paola Caballero and Anthony Hernandez Scientific ManuscriptPaola Caballero and Anthony Hernandez Scientific Manuscript
Paola Caballero and Anthony Hernandez Scientific Manuscript
 
Diversity of hymenopteran parasitoids (Hymenoptera: Chalcididae) associated w...
Diversity of hymenopteran parasitoids (Hymenoptera: Chalcididae) associated w...Diversity of hymenopteran parasitoids (Hymenoptera: Chalcididae) associated w...
Diversity of hymenopteran parasitoids (Hymenoptera: Chalcididae) associated w...
 
4.monica 13.felix phages report corrected
4.monica 13.felix phages report corrected4.monica 13.felix phages report corrected
4.monica 13.felix phages report corrected
 
Poster_TR_07.09.16_RM_V2_FINAL
Poster_TR_07.09.16_RM_V2_FINALPoster_TR_07.09.16_RM_V2_FINAL
Poster_TR_07.09.16_RM_V2_FINAL
 
CHARACTERISING FOOT-AND-MOUTH DISEASE VIRUS IN CLINICAL SAMPLES USING NANOPOR...
CHARACTERISING FOOT-AND-MOUTH DISEASE VIRUS IN CLINICAL SAMPLES USING NANOPOR...CHARACTERISING FOOT-AND-MOUTH DISEASE VIRUS IN CLINICAL SAMPLES USING NANOPOR...
CHARACTERISING FOOT-AND-MOUTH DISEASE VIRUS IN CLINICAL SAMPLES USING NANOPOR...
 
final report
final reportfinal report
final report
 
Development and anlysis of a 20K SNP array for potato (Solanum tuberosum) an ...
Development and anlysis of a 20K SNP array for potato (Solanum tuberosum) an ...Development and anlysis of a 20K SNP array for potato (Solanum tuberosum) an ...
Development and anlysis of a 20K SNP array for potato (Solanum tuberosum) an ...
 
QTL Mapping of Cotton Verticillium Wilt Resistance
QTL Mapping of Cotton Verticillium Wilt ResistanceQTL Mapping of Cotton Verticillium Wilt Resistance
QTL Mapping of Cotton Verticillium Wilt Resistance
 
1-s2.0-S0923250811001872-main
1-s2.0-S0923250811001872-main1-s2.0-S0923250811001872-main
1-s2.0-S0923250811001872-main
 
Victor Poster FINAL PDF
Victor Poster FINAL PDFVictor Poster FINAL PDF
Victor Poster FINAL PDF
 
Molecular study of fasciola spp
Molecular study of fasciola sppMolecular study of fasciola spp
Molecular study of fasciola spp
 
Poster tuber borchii research in portugal iwemm morocco
Poster tuber borchii research in portugal iwemm moroccoPoster tuber borchii research in portugal iwemm morocco
Poster tuber borchii research in portugal iwemm morocco
 
Antimicrobial Activity of Actinomycetes from Soil Samples of Some 6(35)April ...
Antimicrobial Activity of Actinomycetes from Soil Samples of Some 6(35)April ...Antimicrobial Activity of Actinomycetes from Soil Samples of Some 6(35)April ...
Antimicrobial Activity of Actinomycetes from Soil Samples of Some 6(35)April ...
 
9 diversity of meloidogyne in martinique
9 diversity of meloidogyne in martinique9 diversity of meloidogyne in martinique
9 diversity of meloidogyne in martinique
 
Genetic Diversity of Sorghum (Sorghum bicolor L. Moench) from East and West H...
Genetic Diversity of Sorghum (Sorghum bicolor L. Moench) from East and West H...Genetic Diversity of Sorghum (Sorghum bicolor L. Moench) from East and West H...
Genetic Diversity of Sorghum (Sorghum bicolor L. Moench) from East and West H...
 
TESS PURC Poster
TESS PURC PosterTESS PURC Poster
TESS PURC Poster
 
DEVELOPMENT OF LINEAGE-SPECIFIC REAL-TIME RT-PCR FOR THE RECENT FMDV, ASIA/SE...
DEVELOPMENT OF LINEAGE-SPECIFIC REAL-TIME RT-PCR FOR THE RECENT FMDV, ASIA/SE...DEVELOPMENT OF LINEAGE-SPECIFIC REAL-TIME RT-PCR FOR THE RECENT FMDV, ASIA/SE...
DEVELOPMENT OF LINEAGE-SPECIFIC REAL-TIME RT-PCR FOR THE RECENT FMDV, ASIA/SE...
 
READY-TO-USE SOLID PHASE BLOCKING ELISA KITS FOR DETECTION OF SPECIFIC ANTIBO...
READY-TO-USE SOLID PHASE BLOCKING ELISA KITS FOR DETECTION OF SPECIFIC ANTIBO...READY-TO-USE SOLID PHASE BLOCKING ELISA KITS FOR DETECTION OF SPECIFIC ANTIBO...
READY-TO-USE SOLID PHASE BLOCKING ELISA KITS FOR DETECTION OF SPECIFIC ANTIBO...
 

Similar to Vilanova et al tuber 2013 characterisation of pseuromonadaceae populations in tuber melanosporum wild truffieres

Assessment of Endophytic Fungal Flora Responsible for Plant Growth Promotion...
Assessment of Endophytic Fungal Flora Responsible for Plant  Growth Promotion...Assessment of Endophytic Fungal Flora Responsible for Plant  Growth Promotion...
Assessment of Endophytic Fungal Flora Responsible for Plant Growth Promotion...
Sryahwa Publications
 
Study on mycoflora associated with
Study on mycoflora associated withStudy on mycoflora associated with
Study on mycoflora associated with
ijabjournal
 
Population dynamics of ground dwelling spider genera among mustard crop
Population dynamics of ground dwelling spider genera among mustard cropPopulation dynamics of ground dwelling spider genera among mustard crop
Population dynamics of ground dwelling spider genera among mustard crop
Innspub Net
 
Community composition and species diversity of fruit-eating- insects of Gymna...
Community composition and species diversity of fruit-eating- insects of Gymna...Community composition and species diversity of fruit-eating- insects of Gymna...
Community composition and species diversity of fruit-eating- insects of Gymna...
International Multispeciality Journal of Health
 
Examining the spatial distribution pattern and optimum sample size for monito...
Examining the spatial distribution pattern and optimum sample size for monito...Examining the spatial distribution pattern and optimum sample size for monito...
Examining the spatial distribution pattern and optimum sample size for monito...
AI Publications
 
Genetic Relatedness among Six Common Phoenix dactylifera (Palmae) Cultivars i...
Genetic Relatedness among Six Common Phoenix dactylifera (Palmae) Cultivars i...Genetic Relatedness among Six Common Phoenix dactylifera (Palmae) Cultivars i...
Genetic Relatedness among Six Common Phoenix dactylifera (Palmae) Cultivars i...
the Egyptian Society for Environmental Sciences
 
Assessment of Genetic Diversity in 13 Local Banana (Musa Spp.) Cultivars Usin...
Assessment of Genetic Diversity in 13 Local Banana (Musa Spp.) Cultivars Usin...Assessment of Genetic Diversity in 13 Local Banana (Musa Spp.) Cultivars Usin...
Assessment of Genetic Diversity in 13 Local Banana (Musa Spp.) Cultivars Usin...
paperpublications3
 
Effects of Cropping Patterns on the Flea Beetles, Podagrica Spp. (Coleoptera:...
Effects of Cropping Patterns on the Flea Beetles, Podagrica Spp. (Coleoptera:...Effects of Cropping Patterns on the Flea Beetles, Podagrica Spp. (Coleoptera:...
Effects of Cropping Patterns on the Flea Beetles, Podagrica Spp. (Coleoptera:...
Journal of Agriculture and Crops
 
DETERMINATIONS OF IMIDACLOPRID AND NANO- IMIDACLOPRID ON SOME TOMATO SERIOUS ...
DETERMINATIONS OF IMIDACLOPRID AND NANO- IMIDACLOPRID ON SOME TOMATO SERIOUS ...DETERMINATIONS OF IMIDACLOPRID AND NANO- IMIDACLOPRID ON SOME TOMATO SERIOUS ...
DETERMINATIONS OF IMIDACLOPRID AND NANO- IMIDACLOPRID ON SOME TOMATO SERIOUS ...
International Educational Applied Scientific Research Journal (IEASRJ)
 
Verticillium1996
Verticillium1996Verticillium1996
Verticillium1996
Wolfgang Schweigkofler
 
Effect of Different Weed Control Practices On Proximate Composition, Nutrient...
Effect of Different Weed Control Practices On Proximate Composition, Nutrient...Effect of Different Weed Control Practices On Proximate Composition, Nutrient...
Effect of Different Weed Control Practices On Proximate Composition, Nutrient...
IOSRJAVS
 
Deinococcus.full
Deinococcus.fullDeinococcus.full
Deinococcus.full
Patrick McTernan
 
5 mohammad chamani
5 mohammad chamani5 mohammad chamani
5 mohammad chamani
Dheeraj Vasu
 
Distribution of ground dwelling spider genera among berseem crop at Okara dis...
Distribution of ground dwelling spider genera among berseem crop at Okara dis...Distribution of ground dwelling spider genera among berseem crop at Okara dis...
Distribution of ground dwelling spider genera among berseem crop at Okara dis...
Innspub Net
 
Characterization of Arsenic contaminated Rice (Oryza Sativa L.) through RAPD ...
Characterization of Arsenic contaminated Rice (Oryza Sativa L.) through RAPD ...Characterization of Arsenic contaminated Rice (Oryza Sativa L.) through RAPD ...
Characterization of Arsenic contaminated Rice (Oryza Sativa L.) through RAPD ...
IOSR Journals
 
Characterization of Arsenic contaminated Rice (Oryza Sativa L.) through RAPD ...
Characterization of Arsenic contaminated Rice (Oryza Sativa L.) through RAPD ...Characterization of Arsenic contaminated Rice (Oryza Sativa L.) through RAPD ...
Characterization of Arsenic contaminated Rice (Oryza Sativa L.) through RAPD ...
IOSR Journals
 
Aijrfans14 215
Aijrfans14 215Aijrfans14 215
Aijrfans14 215
Iasir Journals
 
AM Fungal Status in Ketaka: Pandanus fascicularis From Coastal Region of Konk...
AM Fungal Status in Ketaka: Pandanus fascicularis From Coastal Region of Konk...AM Fungal Status in Ketaka: Pandanus fascicularis From Coastal Region of Konk...
AM Fungal Status in Ketaka: Pandanus fascicularis From Coastal Region of Konk...
IOSR Journals
 
A0560106
A0560106A0560106
A0560106
IOSR Journals
 
A0560106
A0560106A0560106
A0560106
IOSR Journals
 

Similar to Vilanova et al tuber 2013 characterisation of pseuromonadaceae populations in tuber melanosporum wild truffieres (20)

Assessment of Endophytic Fungal Flora Responsible for Plant Growth Promotion...
Assessment of Endophytic Fungal Flora Responsible for Plant  Growth Promotion...Assessment of Endophytic Fungal Flora Responsible for Plant  Growth Promotion...
Assessment of Endophytic Fungal Flora Responsible for Plant Growth Promotion...
 
Study on mycoflora associated with
Study on mycoflora associated withStudy on mycoflora associated with
Study on mycoflora associated with
 
Population dynamics of ground dwelling spider genera among mustard crop
Population dynamics of ground dwelling spider genera among mustard cropPopulation dynamics of ground dwelling spider genera among mustard crop
Population dynamics of ground dwelling spider genera among mustard crop
 
Community composition and species diversity of fruit-eating- insects of Gymna...
Community composition and species diversity of fruit-eating- insects of Gymna...Community composition and species diversity of fruit-eating- insects of Gymna...
Community composition and species diversity of fruit-eating- insects of Gymna...
 
Examining the spatial distribution pattern and optimum sample size for monito...
Examining the spatial distribution pattern and optimum sample size for monito...Examining the spatial distribution pattern and optimum sample size for monito...
Examining the spatial distribution pattern and optimum sample size for monito...
 
Genetic Relatedness among Six Common Phoenix dactylifera (Palmae) Cultivars i...
Genetic Relatedness among Six Common Phoenix dactylifera (Palmae) Cultivars i...Genetic Relatedness among Six Common Phoenix dactylifera (Palmae) Cultivars i...
Genetic Relatedness among Six Common Phoenix dactylifera (Palmae) Cultivars i...
 
Assessment of Genetic Diversity in 13 Local Banana (Musa Spp.) Cultivars Usin...
Assessment of Genetic Diversity in 13 Local Banana (Musa Spp.) Cultivars Usin...Assessment of Genetic Diversity in 13 Local Banana (Musa Spp.) Cultivars Usin...
Assessment of Genetic Diversity in 13 Local Banana (Musa Spp.) Cultivars Usin...
 
Effects of Cropping Patterns on the Flea Beetles, Podagrica Spp. (Coleoptera:...
Effects of Cropping Patterns on the Flea Beetles, Podagrica Spp. (Coleoptera:...Effects of Cropping Patterns on the Flea Beetles, Podagrica Spp. (Coleoptera:...
Effects of Cropping Patterns on the Flea Beetles, Podagrica Spp. (Coleoptera:...
 
DETERMINATIONS OF IMIDACLOPRID AND NANO- IMIDACLOPRID ON SOME TOMATO SERIOUS ...
DETERMINATIONS OF IMIDACLOPRID AND NANO- IMIDACLOPRID ON SOME TOMATO SERIOUS ...DETERMINATIONS OF IMIDACLOPRID AND NANO- IMIDACLOPRID ON SOME TOMATO SERIOUS ...
DETERMINATIONS OF IMIDACLOPRID AND NANO- IMIDACLOPRID ON SOME TOMATO SERIOUS ...
 
Verticillium1996
Verticillium1996Verticillium1996
Verticillium1996
 
Effect of Different Weed Control Practices On Proximate Composition, Nutrient...
Effect of Different Weed Control Practices On Proximate Composition, Nutrient...Effect of Different Weed Control Practices On Proximate Composition, Nutrient...
Effect of Different Weed Control Practices On Proximate Composition, Nutrient...
 
Deinococcus.full
Deinococcus.fullDeinococcus.full
Deinococcus.full
 
5 mohammad chamani
5 mohammad chamani5 mohammad chamani
5 mohammad chamani
 
Distribution of ground dwelling spider genera among berseem crop at Okara dis...
Distribution of ground dwelling spider genera among berseem crop at Okara dis...Distribution of ground dwelling spider genera among berseem crop at Okara dis...
Distribution of ground dwelling spider genera among berseem crop at Okara dis...
 
Characterization of Arsenic contaminated Rice (Oryza Sativa L.) through RAPD ...
Characterization of Arsenic contaminated Rice (Oryza Sativa L.) through RAPD ...Characterization of Arsenic contaminated Rice (Oryza Sativa L.) through RAPD ...
Characterization of Arsenic contaminated Rice (Oryza Sativa L.) through RAPD ...
 
Characterization of Arsenic contaminated Rice (Oryza Sativa L.) through RAPD ...
Characterization of Arsenic contaminated Rice (Oryza Sativa L.) through RAPD ...Characterization of Arsenic contaminated Rice (Oryza Sativa L.) through RAPD ...
Characterization of Arsenic contaminated Rice (Oryza Sativa L.) through RAPD ...
 
Aijrfans14 215
Aijrfans14 215Aijrfans14 215
Aijrfans14 215
 
AM Fungal Status in Ketaka: Pandanus fascicularis From Coastal Region of Konk...
AM Fungal Status in Ketaka: Pandanus fascicularis From Coastal Region of Konk...AM Fungal Status in Ketaka: Pandanus fascicularis From Coastal Region of Konk...
AM Fungal Status in Ketaka: Pandanus fascicularis From Coastal Region of Konk...
 
A0560106
A0560106A0560106
A0560106
 
A0560106
A0560106A0560106
A0560106
 

More from Micologia Forestal & Aplicada

Truffle farming guide micofora booklet
Truffle farming guide   micofora bookletTruffle farming guide   micofora booklet
Truffle farming guide micofora booklet
Micologia Forestal & Aplicada
 
Viabilidad y rentabilidad de una plantación de pino piñonero micorrizado co...
Viabilidad y rentabilidad de una plantación de pino piñonero micorrizado co...Viabilidad y rentabilidad de una plantación de pino piñonero micorrizado co...
Viabilidad y rentabilidad de una plantación de pino piñonero micorrizado co...
Micologia Forestal & Aplicada
 
Lecture xavi vilanova effcet of bacteria on truffle mycelium growth irta mic...
Lecture xavi vilanova effcet of bacteria on truffle mycelium growth irta  mic...Lecture xavi vilanova effcet of bacteria on truffle mycelium growth irta  mic...
Lecture xavi vilanova effcet of bacteria on truffle mycelium growth irta mic...
Micologia Forestal & Aplicada
 
Xerrada efecte bacteris en la producció de tòfones Irta Micofora 2017
Xerrada efecte bacteris en la producció de tòfones Irta Micofora 2017Xerrada efecte bacteris en la producció de tòfones Irta Micofora 2017
Xerrada efecte bacteris en la producció de tòfones Irta Micofora 2017
Micologia Forestal & Aplicada
 
Ponencia quantificació miceli tòfona negra al sòl i biodiversitat en tofon...
Ponencia quantificació miceli tòfona negra al sòl i biodiversitat en tofon...Ponencia quantificació miceli tòfona negra al sòl i biodiversitat en tofon...
Ponencia quantificació miceli tòfona negra al sòl i biodiversitat en tofon...
Micologia Forestal & Aplicada
 
Hongos silvestres en guatemala
Hongos silvestres en guatemalaHongos silvestres en guatemala
Hongos silvestres en guatemala
Micologia Forestal & Aplicada
 
Cultivo de hongos y desarrollo rural en nicaragua
Cultivo de hongos y desarrollo rural en nicaraguaCultivo de hongos y desarrollo rural en nicaragua
Cultivo de hongos y desarrollo rural en nicaragua
Micologia Forestal & Aplicada
 
Cómo tomar muestras de suelo
Cómo tomar muestras de sueloCómo tomar muestras de suelo
Cómo tomar muestras de suelo
Micologia Forestal & Aplicada
 
¿Por qué es tan difícil cultivar hongos micorrícicos comestibles?
¿Por qué es tan difícil cultivar hongos micorrícicos comestibles?¿Por qué es tan difícil cultivar hongos micorrícicos comestibles?
¿Por qué es tan difícil cultivar hongos micorrícicos comestibles?
Micologia Forestal & Aplicada
 
Barcelona Truffle Tour notes January 2016
Barcelona Truffle Tour notes January 2016Barcelona Truffle Tour notes January 2016
Barcelona Truffle Tour notes January 2016
Micologia Forestal & Aplicada
 
Jornada tòfones i tubericultura IRTA caldes montbui
Jornada tòfones i tubericultura IRTA caldes montbuiJornada tòfones i tubericultura IRTA caldes montbui
Jornada tòfones i tubericultura IRTA caldes montbui
Micologia Forestal & Aplicada
 
Cape kaliakra moments 2015 interview bulgaria
Cape kaliakra moments 2015 interview bulgariaCape kaliakra moments 2015 interview bulgaria
Cape kaliakra moments 2015 interview bulgaria
Micologia Forestal & Aplicada
 
Barcelona truffle-tour-2015-16
Barcelona truffle-tour-2015-16Barcelona truffle-tour-2015-16
Barcelona truffle-tour-2015-16
Micologia Forestal & Aplicada
 
Cambios de paradigma en truficultura
Cambios de paradigma en truficultura Cambios de paradigma en truficultura
Cambios de paradigma en truficultura
Micologia Forestal & Aplicada
 
Ponencia marcos morcillo cultiu tuber borchii
Ponencia marcos morcillo   cultiu tuber borchiiPonencia marcos morcillo   cultiu tuber borchii
Ponencia marcos morcillo cultiu tuber borchii
Micologia Forestal & Aplicada
 
1 cultivo de setas micorrícicas comestibles 2014 marcos morcillo
1  cultivo de setas micorrícicas comestibles 2014 marcos morcillo1  cultivo de setas micorrícicas comestibles 2014 marcos morcillo
1 cultivo de setas micorrícicas comestibles 2014 marcos morcillo
Micologia Forestal & Aplicada
 
2 cultivo de la trufa en el mundo 2014 marcos morcillo
2  cultivo de la trufa en el mundo 2014 marcos morcillo2  cultivo de la trufa en el mundo 2014 marcos morcillo
2 cultivo de la trufa en el mundo 2014 marcos morcillo
Micologia Forestal & Aplicada
 
3 cultivo de tuber aestivum, uncinatum, borchii y brumale 2014 marcos morcillo
3  cultivo de tuber aestivum, uncinatum, borchii y brumale 2014 marcos morcillo3  cultivo de tuber aestivum, uncinatum, borchii y brumale 2014 marcos morcillo
3 cultivo de tuber aestivum, uncinatum, borchii y brumale 2014 marcos morcillo
Micologia Forestal & Aplicada
 
4 mercado trufas oferta -demanda y cadena valor 2014 marcos morcillo
4  mercado trufas oferta -demanda y cadena valor 2014 marcos morcillo4  mercado trufas oferta -demanda y cadena valor 2014 marcos morcillo
4 mercado trufas oferta -demanda y cadena valor 2014 marcos morcillo
Micologia Forestal & Aplicada
 
Poster morcillo et al tuber borchii micorrhization depending on host tree a...
Poster morcillo et al   tuber borchii micorrhization depending on host tree a...Poster morcillo et al   tuber borchii micorrhization depending on host tree a...
Poster morcillo et al tuber borchii micorrhization depending on host tree a...
Micologia Forestal & Aplicada
 

More from Micologia Forestal & Aplicada (20)

Truffle farming guide micofora booklet
Truffle farming guide   micofora bookletTruffle farming guide   micofora booklet
Truffle farming guide micofora booklet
 
Viabilidad y rentabilidad de una plantación de pino piñonero micorrizado co...
Viabilidad y rentabilidad de una plantación de pino piñonero micorrizado co...Viabilidad y rentabilidad de una plantación de pino piñonero micorrizado co...
Viabilidad y rentabilidad de una plantación de pino piñonero micorrizado co...
 
Lecture xavi vilanova effcet of bacteria on truffle mycelium growth irta mic...
Lecture xavi vilanova effcet of bacteria on truffle mycelium growth irta  mic...Lecture xavi vilanova effcet of bacteria on truffle mycelium growth irta  mic...
Lecture xavi vilanova effcet of bacteria on truffle mycelium growth irta mic...
 
Xerrada efecte bacteris en la producció de tòfones Irta Micofora 2017
Xerrada efecte bacteris en la producció de tòfones Irta Micofora 2017Xerrada efecte bacteris en la producció de tòfones Irta Micofora 2017
Xerrada efecte bacteris en la producció de tòfones Irta Micofora 2017
 
Ponencia quantificació miceli tòfona negra al sòl i biodiversitat en tofon...
Ponencia quantificació miceli tòfona negra al sòl i biodiversitat en tofon...Ponencia quantificació miceli tòfona negra al sòl i biodiversitat en tofon...
Ponencia quantificació miceli tòfona negra al sòl i biodiversitat en tofon...
 
Hongos silvestres en guatemala
Hongos silvestres en guatemalaHongos silvestres en guatemala
Hongos silvestres en guatemala
 
Cultivo de hongos y desarrollo rural en nicaragua
Cultivo de hongos y desarrollo rural en nicaraguaCultivo de hongos y desarrollo rural en nicaragua
Cultivo de hongos y desarrollo rural en nicaragua
 
Cómo tomar muestras de suelo
Cómo tomar muestras de sueloCómo tomar muestras de suelo
Cómo tomar muestras de suelo
 
¿Por qué es tan difícil cultivar hongos micorrícicos comestibles?
¿Por qué es tan difícil cultivar hongos micorrícicos comestibles?¿Por qué es tan difícil cultivar hongos micorrícicos comestibles?
¿Por qué es tan difícil cultivar hongos micorrícicos comestibles?
 
Barcelona Truffle Tour notes January 2016
Barcelona Truffle Tour notes January 2016Barcelona Truffle Tour notes January 2016
Barcelona Truffle Tour notes January 2016
 
Jornada tòfones i tubericultura IRTA caldes montbui
Jornada tòfones i tubericultura IRTA caldes montbuiJornada tòfones i tubericultura IRTA caldes montbui
Jornada tòfones i tubericultura IRTA caldes montbui
 
Cape kaliakra moments 2015 interview bulgaria
Cape kaliakra moments 2015 interview bulgariaCape kaliakra moments 2015 interview bulgaria
Cape kaliakra moments 2015 interview bulgaria
 
Barcelona truffle-tour-2015-16
Barcelona truffle-tour-2015-16Barcelona truffle-tour-2015-16
Barcelona truffle-tour-2015-16
 
Cambios de paradigma en truficultura
Cambios de paradigma en truficultura Cambios de paradigma en truficultura
Cambios de paradigma en truficultura
 
Ponencia marcos morcillo cultiu tuber borchii
Ponencia marcos morcillo   cultiu tuber borchiiPonencia marcos morcillo   cultiu tuber borchii
Ponencia marcos morcillo cultiu tuber borchii
 
1 cultivo de setas micorrícicas comestibles 2014 marcos morcillo
1  cultivo de setas micorrícicas comestibles 2014 marcos morcillo1  cultivo de setas micorrícicas comestibles 2014 marcos morcillo
1 cultivo de setas micorrícicas comestibles 2014 marcos morcillo
 
2 cultivo de la trufa en el mundo 2014 marcos morcillo
2  cultivo de la trufa en el mundo 2014 marcos morcillo2  cultivo de la trufa en el mundo 2014 marcos morcillo
2 cultivo de la trufa en el mundo 2014 marcos morcillo
 
3 cultivo de tuber aestivum, uncinatum, borchii y brumale 2014 marcos morcillo
3  cultivo de tuber aestivum, uncinatum, borchii y brumale 2014 marcos morcillo3  cultivo de tuber aestivum, uncinatum, borchii y brumale 2014 marcos morcillo
3 cultivo de tuber aestivum, uncinatum, borchii y brumale 2014 marcos morcillo
 
4 mercado trufas oferta -demanda y cadena valor 2014 marcos morcillo
4  mercado trufas oferta -demanda y cadena valor 2014 marcos morcillo4  mercado trufas oferta -demanda y cadena valor 2014 marcos morcillo
4 mercado trufas oferta -demanda y cadena valor 2014 marcos morcillo
 
Poster morcillo et al tuber borchii micorrhization depending on host tree a...
Poster morcillo et al   tuber borchii micorrhization depending on host tree a...Poster morcillo et al   tuber borchii micorrhization depending on host tree a...
Poster morcillo et al tuber borchii micorrhization depending on host tree a...
 

Recently uploaded

Energy Efficient Video Encoding for Cloud and Edge Computing Instances
Energy Efficient Video Encoding for Cloud and Edge Computing InstancesEnergy Efficient Video Encoding for Cloud and Edge Computing Instances
Energy Efficient Video Encoding for Cloud and Edge Computing Instances
Alpen-Adria-Universität
 
WeTestAthens: Postman's AI & Automation Techniques
WeTestAthens: Postman's AI & Automation TechniquesWeTestAthens: Postman's AI & Automation Techniques
WeTestAthens: Postman's AI & Automation Techniques
Postman
 
Presentation of the OECD Artificial Intelligence Review of Germany
Presentation of the OECD Artificial Intelligence Review of GermanyPresentation of the OECD Artificial Intelligence Review of Germany
Presentation of the OECD Artificial Intelligence Review of Germany
innovationoecd
 
Generating privacy-protected synthetic data using Secludy and Milvus
Generating privacy-protected synthetic data using Secludy and MilvusGenerating privacy-protected synthetic data using Secludy and Milvus
Generating privacy-protected synthetic data using Secludy and Milvus
Zilliz
 
Digital Banking in the Cloud: How Citizens Bank Unlocked Their Mainframe
Digital Banking in the Cloud: How Citizens Bank Unlocked Their MainframeDigital Banking in the Cloud: How Citizens Bank Unlocked Their Mainframe
Digital Banking in the Cloud: How Citizens Bank Unlocked Their Mainframe
Precisely
 
Salesforce Integration for Bonterra Impact Management (fka Social Solutions A...
Salesforce Integration for Bonterra Impact Management (fka Social Solutions A...Salesforce Integration for Bonterra Impact Management (fka Social Solutions A...
Salesforce Integration for Bonterra Impact Management (fka Social Solutions A...
Jeffrey Haguewood
 
Best 20 SEO Techniques To Improve Website Visibility In SERP
Best 20 SEO Techniques To Improve Website Visibility In SERPBest 20 SEO Techniques To Improve Website Visibility In SERP
Best 20 SEO Techniques To Improve Website Visibility In SERP
Pixlogix Infotech
 
Building Production Ready Search Pipelines with Spark and Milvus
Building Production Ready Search Pipelines with Spark and MilvusBuilding Production Ready Search Pipelines with Spark and Milvus
Building Production Ready Search Pipelines with Spark and Milvus
Zilliz
 
Programming Foundation Models with DSPy - Meetup Slides
Programming Foundation Models with DSPy - Meetup SlidesProgramming Foundation Models with DSPy - Meetup Slides
Programming Foundation Models with DSPy - Meetup Slides
Zilliz
 
Your One-Stop Shop for Python Success: Top 10 US Python Development Providers
Your One-Stop Shop for Python Success: Top 10 US Python Development ProvidersYour One-Stop Shop for Python Success: Top 10 US Python Development Providers
Your One-Stop Shop for Python Success: Top 10 US Python Development Providers
akankshawande
 
5th LF Energy Power Grid Model Meet-up Slides
5th LF Energy Power Grid Model Meet-up Slides5th LF Energy Power Grid Model Meet-up Slides
5th LF Energy Power Grid Model Meet-up Slides
DanBrown980551
 
zkStudyClub - LatticeFold: A Lattice-based Folding Scheme and its Application...
zkStudyClub - LatticeFold: A Lattice-based Folding Scheme and its Application...zkStudyClub - LatticeFold: A Lattice-based Folding Scheme and its Application...
zkStudyClub - LatticeFold: A Lattice-based Folding Scheme and its Application...
Alex Pruden
 
FREE A4 Cyber Security Awareness Posters-Social Engineering part 3
FREE A4 Cyber Security Awareness  Posters-Social Engineering part 3FREE A4 Cyber Security Awareness  Posters-Social Engineering part 3
FREE A4 Cyber Security Awareness Posters-Social Engineering part 3
Data Hops
 
Astute Business Solutions | Oracle Cloud Partner |
Astute Business Solutions | Oracle Cloud Partner |Astute Business Solutions | Oracle Cloud Partner |
Astute Business Solutions | Oracle Cloud Partner |
AstuteBusiness
 
Choosing The Best AWS Service For Your Website + API.pptx
Choosing The Best AWS Service For Your Website + API.pptxChoosing The Best AWS Service For Your Website + API.pptx
Choosing The Best AWS Service For Your Website + API.pptx
Brandon Minnick, MBA
 
Main news related to the CCS TSI 2023 (2023/1695)
Main news related to the CCS TSI 2023 (2023/1695)Main news related to the CCS TSI 2023 (2023/1695)
Main news related to the CCS TSI 2023 (2023/1695)
Jakub Marek
 
Let's Integrate MuleSoft RPA, COMPOSER, APM with AWS IDP along with Slack
Let's Integrate MuleSoft RPA, COMPOSER, APM with AWS IDP along with SlackLet's Integrate MuleSoft RPA, COMPOSER, APM with AWS IDP along with Slack
Let's Integrate MuleSoft RPA, COMPOSER, APM with AWS IDP along with Slack
shyamraj55
 
A Comprehensive Guide to DeFi Development Services in 2024
A Comprehensive Guide to DeFi Development Services in 2024A Comprehensive Guide to DeFi Development Services in 2024
A Comprehensive Guide to DeFi Development Services in 2024
Intelisync
 
How to Interpret Trends in the Kalyan Rajdhani Mix Chart.pdf
How to Interpret Trends in the Kalyan Rajdhani Mix Chart.pdfHow to Interpret Trends in the Kalyan Rajdhani Mix Chart.pdf
How to Interpret Trends in the Kalyan Rajdhani Mix Chart.pdf
Chart Kalyan
 
Azure API Management to expose backend services securely
Azure API Management to expose backend services securelyAzure API Management to expose backend services securely
Azure API Management to expose backend services securely
Dinusha Kumarasiri
 

Recently uploaded (20)

Energy Efficient Video Encoding for Cloud and Edge Computing Instances
Energy Efficient Video Encoding for Cloud and Edge Computing InstancesEnergy Efficient Video Encoding for Cloud and Edge Computing Instances
Energy Efficient Video Encoding for Cloud and Edge Computing Instances
 
WeTestAthens: Postman's AI & Automation Techniques
WeTestAthens: Postman's AI & Automation TechniquesWeTestAthens: Postman's AI & Automation Techniques
WeTestAthens: Postman's AI & Automation Techniques
 
Presentation of the OECD Artificial Intelligence Review of Germany
Presentation of the OECD Artificial Intelligence Review of GermanyPresentation of the OECD Artificial Intelligence Review of Germany
Presentation of the OECD Artificial Intelligence Review of Germany
 
Generating privacy-protected synthetic data using Secludy and Milvus
Generating privacy-protected synthetic data using Secludy and MilvusGenerating privacy-protected synthetic data using Secludy and Milvus
Generating privacy-protected synthetic data using Secludy and Milvus
 
Digital Banking in the Cloud: How Citizens Bank Unlocked Their Mainframe
Digital Banking in the Cloud: How Citizens Bank Unlocked Their MainframeDigital Banking in the Cloud: How Citizens Bank Unlocked Their Mainframe
Digital Banking in the Cloud: How Citizens Bank Unlocked Their Mainframe
 
Salesforce Integration for Bonterra Impact Management (fka Social Solutions A...
Salesforce Integration for Bonterra Impact Management (fka Social Solutions A...Salesforce Integration for Bonterra Impact Management (fka Social Solutions A...
Salesforce Integration for Bonterra Impact Management (fka Social Solutions A...
 
Best 20 SEO Techniques To Improve Website Visibility In SERP
Best 20 SEO Techniques To Improve Website Visibility In SERPBest 20 SEO Techniques To Improve Website Visibility In SERP
Best 20 SEO Techniques To Improve Website Visibility In SERP
 
Building Production Ready Search Pipelines with Spark and Milvus
Building Production Ready Search Pipelines with Spark and MilvusBuilding Production Ready Search Pipelines with Spark and Milvus
Building Production Ready Search Pipelines with Spark and Milvus
 
Programming Foundation Models with DSPy - Meetup Slides
Programming Foundation Models with DSPy - Meetup SlidesProgramming Foundation Models with DSPy - Meetup Slides
Programming Foundation Models with DSPy - Meetup Slides
 
Your One-Stop Shop for Python Success: Top 10 US Python Development Providers
Your One-Stop Shop for Python Success: Top 10 US Python Development ProvidersYour One-Stop Shop for Python Success: Top 10 US Python Development Providers
Your One-Stop Shop for Python Success: Top 10 US Python Development Providers
 
5th LF Energy Power Grid Model Meet-up Slides
5th LF Energy Power Grid Model Meet-up Slides5th LF Energy Power Grid Model Meet-up Slides
5th LF Energy Power Grid Model Meet-up Slides
 
zkStudyClub - LatticeFold: A Lattice-based Folding Scheme and its Application...
zkStudyClub - LatticeFold: A Lattice-based Folding Scheme and its Application...zkStudyClub - LatticeFold: A Lattice-based Folding Scheme and its Application...
zkStudyClub - LatticeFold: A Lattice-based Folding Scheme and its Application...
 
FREE A4 Cyber Security Awareness Posters-Social Engineering part 3
FREE A4 Cyber Security Awareness  Posters-Social Engineering part 3FREE A4 Cyber Security Awareness  Posters-Social Engineering part 3
FREE A4 Cyber Security Awareness Posters-Social Engineering part 3
 
Astute Business Solutions | Oracle Cloud Partner |
Astute Business Solutions | Oracle Cloud Partner |Astute Business Solutions | Oracle Cloud Partner |
Astute Business Solutions | Oracle Cloud Partner |
 
Choosing The Best AWS Service For Your Website + API.pptx
Choosing The Best AWS Service For Your Website + API.pptxChoosing The Best AWS Service For Your Website + API.pptx
Choosing The Best AWS Service For Your Website + API.pptx
 
Main news related to the CCS TSI 2023 (2023/1695)
Main news related to the CCS TSI 2023 (2023/1695)Main news related to the CCS TSI 2023 (2023/1695)
Main news related to the CCS TSI 2023 (2023/1695)
 
Let's Integrate MuleSoft RPA, COMPOSER, APM with AWS IDP along with Slack
Let's Integrate MuleSoft RPA, COMPOSER, APM with AWS IDP along with SlackLet's Integrate MuleSoft RPA, COMPOSER, APM with AWS IDP along with Slack
Let's Integrate MuleSoft RPA, COMPOSER, APM with AWS IDP along with Slack
 
A Comprehensive Guide to DeFi Development Services in 2024
A Comprehensive Guide to DeFi Development Services in 2024A Comprehensive Guide to DeFi Development Services in 2024
A Comprehensive Guide to DeFi Development Services in 2024
 
How to Interpret Trends in the Kalyan Rajdhani Mix Chart.pdf
How to Interpret Trends in the Kalyan Rajdhani Mix Chart.pdfHow to Interpret Trends in the Kalyan Rajdhani Mix Chart.pdf
How to Interpret Trends in the Kalyan Rajdhani Mix Chart.pdf
 
Azure API Management to expose backend services securely
Azure API Management to expose backend services securelyAzure API Management to expose backend services securely
Azure API Management to expose backend services securely
 

Vilanova et al tuber 2013 characterisation of pseuromonadaceae populations in tuber melanosporum wild truffieres

  • 1. Characterisation of Pseudomonadaceae populations in Tuber melanosporum wild truffieres Vilanova, X1*.; Sanchez, M1.; Viñas, M2. Morcillo, M. 1 1* Micología Forestal & Aplicada S. L. Address* Rambla Arnau de Vilanova , 6 local D 08800 Vilanova i la Geltrú (Barcelona-Spain). E-mail* xaviervilanova@micofora.com URL* www.micofora.com 2 Giro Joint Research Unit IRTA-UPC, IRTA, Torre Marimon, E-08140-Caldes de Montbui (Barcelona-Spain). Abstract In the last decade several surveys have revealed an important role of Pseudomonas genus as a Mycorrhizal Helper Bacteria (MHB). Moreover, the bioaugmentation of environmental isolated Pseudomonas strains to the substrate at inoculation time in different plant species throughout mycorrhization assays have been related on the occurrence of a greater number of mycorrhiza of Tuber genus. In this work, a selective culture medium was designed to isolate bacteria belonging to Pseudomonaceae family from soil samples taken from two different wild truffières. The amount of culturable Pseudomonadaceae populations was monitored using the selective medium throughout one year in different wild truffières. A total of eight samples per monitored truffières were taken every 40 days for the two monitored truffières. In the same area, non-truffle producing evergreen oaks were used as negative controls being sampled simultaneously to the monitored truffières. The results revealed both a low diversity of colonial morphology and a similar abundance of culturable Pseudomonadaceae strains on the selective- designed agar medium throughout the one-year survey in both monitored truffières (6.44 x 104 ± 3.54 x 104 -1 -1 CFU · g and 5.71 x 104 ± 3.25 x 104 CFU · g respectively (± standard deviation of n=8). Non-producing trees showed similar colonial morphologies to those found from the truffières, but less abundant (2.54 x 104 ± 2.54 x 104 CFU · g-1of soil (± standard deviation of n=8)). Finally, thirty samples from both truffières and controls were characterized by means of 16SrRNA gene-based PCR-DGGE (Polymerase Chain Reaction (PCR) with a subsequent Denaturing Gradient Gel Electrophoresis (DGGE). We obtained a low diversity (1-4 DGGE bands) in all the studied samples (both truffières and controls). It is noteworthy that most of detected bands migrated mainly in two defined positions from the DGGE gel, corresponding to a two different ribotypes belonging to Pseudomonas chlororaphis and Stenotrophomonas spp. respectively. KEYWORDS Pseudomonas, MHB, Tuber melanosporum, wild truffieres.