SlideShare a Scribd company logo
1 of 15
Download to read offline
EVOLUTION OF TUBER MELANOSPORUM MYCELIUM
CONCENTRATION IN THE SOIL THROUGHOUT THE YEAR AND
EVALUATION OF FUNGAL BIODIVERSITY IN PRODUCTIVE AND NON-
PRODUCTIVE TREES
Marcos Morcillo1, Javier Parladé2, Joan Pera2, Mónica Sanchez1, Xavier Vilanova1
1 MICOLOGIA FORESTAL & APLICADA. Cami Del Fou, s/n 08459 Sant Antoni De
Vilamajor. (Barcelona) SPAIN
2 IRTA. Proteccio Vegetal Sostenible Ctra. de Cabrils, Km 2, 08348 Cabrils
(Barcelona) SPAIN
marcosmorcillo@micofora.com
xavier.parlade@irta.cat
Goals
three main objectives:
1) to evaluate the seasonal dynamics of Tuber melanosporum mycelium in a
plantation
2) to develop and validate a system to reinoculate black truffle in pre-productive
orchards and
3) to determine fungal biodiversity in black truffle truffieres on productive and
non productive trees, and reinoculated trees.
Research sites
Trial Sites
Two field trials with loam soils
2013, 2014
Granollers: 11 year Q. ilex, no irrigation
-  Seasonal mycelium concentration
-  Metagenomic biodiversity
Batea: 4 year old Q. ilex, drip irrigation
-  Seasonal mycelium concentration
-  Metagenomic biodiversity
-  Reinoculation trials
Quantification of soil mycelium biomass (qPCR)
kit Power Soil (MoBio) 0,25 g. Soil.
sondes Taqman-MGB, for T. melanosporum,
Standard curves with known mycelium quantities for each trial site.
Parladé et al. 2013. Mycorrhiza 23:99–106.
Distance to tree
(m)
Concentration of
mycelum mg/g *
0,2 8,090
1,5 0,008
n=6 for all treatments
qPCR seasonal dynamics
n=6 for each curve
Evaluation of fungal diversity
Metagenomic platforms
n=6 for each treatment plus control.
Samples mixed so a single
metagenomic per treatment
(no replicate)
Soil fungal diversity
11 year-old T. melanosporum orchard
Soil fungal diversity
11 year-old T. melanosporum orchard
Reinoculations
& spanish wells are a common practice:
qPCR & biodiversity to check effectiveness
qPCR after reinoculations
Concentration of T. melanosporum mycelium in june.
Control vs. Field inoculated (5g/tree) in april.
Significative differences.
Concentration of T. melanosporum mycelium
Control vs. Field june inoculations
Dose 1, 2 i 3 are 9, 5 i 2 grams of truffle /tree
No significative differences.
METAGENOMICS
biodiversity after reinoculations
Conclusions
•  Drip irrigation system seems not to favour truffle mycelium expansion in soil. 3 logarithms below outside
humidity bubble.
•  Seasonal differences in distribution of black truffle mycelium in the soil. Seasonal pattern dependent from
water or irrigation.
•  Significative more Tuber mycelium concentration and sequences in productive trees.
•  Idem for Scleroderma. Could it be a positive partner specie?
•  April inoculations increase (almost double) Tuber sp. sequences and mycelium concentration in june, as
compared to controls non-inoculated, reaching same levels of sequences than when inoculated in june at
5grams. BUT for agronomic reasons it should be better to inoculate in april, as we mostly do mechanically
with tractors over the brulê, in order not to disturb the active mycelium in june.
•  If possible it would be interesting to do the wells with spores even in february – march so spores could
have enough time to germinate and new males promote fecundations in may.
Can these curves become reference standard curves?
Future
Absolute values from Real Time PCR results hardly comparable between geographic
areas or when sampling and analysis are not done at the same time. But good to compare
treatments from single plantations when sampled and analyzed at the same time.
The results have a direct application in the development of new technologies for truffle
farming:
-  traceability of fungal mycelium
-  methods to increase yields
-  to evaluate the effects of different managements in beneficial microbial communities
for the ecosystem (biodiversity).
As metagenomic results seem to be quantitative, this technique may be useful for studies
on diversity dynamics in a plantation, as well as to identify fungal species associated
(positively or negatively) to Tuber persistence.
marcosmorcillo@micofora.com
xavier.parlade@irta.cat
Project Gencat 3844 Funded by

More Related Content

What's hot

Enhanced fungal resistance in transgenic cotton expressing an endochitinase g...
Enhanced fungal resistance in transgenic cotton expressing an endochitinase g...Enhanced fungal resistance in transgenic cotton expressing an endochitinase g...
Enhanced fungal resistance in transgenic cotton expressing an endochitinase g...Kalyani Rajalingham
 
Folding Biologia
Folding BiologiaFolding Biologia
Folding BiologiaLuz Eugenia
 
ISOLATION AND DETECTION OF MUTANTS
ISOLATION AND DETECTION OF MUTANTSISOLATION AND DETECTION OF MUTANTS
ISOLATION AND DETECTION OF MUTANTSSudhir Gupta
 
KarineHerveCV2015-
KarineHerveCV2015-KarineHerveCV2015-
KarineHerveCV2015-Karine HERVE
 
Isolation of mutant
Isolation of mutantIsolation of mutant
Isolation of mutantshveta arya
 
Rosenbloom_PosterAntibiotics
Rosenbloom_PosterAntibioticsRosenbloom_PosterAntibiotics
Rosenbloom_PosterAntibioticsAaron Rosenbloom
 
The landscape of microbial phenotypic traits and associated genes
The landscape of microbial phenotypic traits and  associated genesThe landscape of microbial phenotypic traits and  associated genes
The landscape of microbial phenotypic traits and associated genesFran Supek
 
Design and Evaluation of a 16S-based Integrated Solution to Study Bacterial D...
Design and Evaluation of a 16S-based Integrated Solution to Study Bacterial D...Design and Evaluation of a 16S-based Integrated Solution to Study Bacterial D...
Design and Evaluation of a 16S-based Integrated Solution to Study Bacterial D...Thermo Fisher Scientific
 
Phage typing
Phage typingPhage typing
Phage typingsiva ni
 
Isolation of antibiotic resistance mutant by replica plating
Isolation of antibiotic resistance mutant by replica platingIsolation of antibiotic resistance mutant by replica plating
Isolation of antibiotic resistance mutant by replica platingbenazeer fathima
 
Franz et al tdwg 2016 introducing lep net
Franz et al tdwg 2016 introducing lep netFranz et al tdwg 2016 introducing lep net
Franz et al tdwg 2016 introducing lep nettaxonbytes
 
Isolation of mutants
Isolation of mutantsIsolation of mutants
Isolation of mutantsshveta arya
 

What's hot (20)

Abstract
AbstractAbstract
Abstract
 
Enhanced fungal resistance in transgenic cotton expressing an endochitinase g...
Enhanced fungal resistance in transgenic cotton expressing an endochitinase g...Enhanced fungal resistance in transgenic cotton expressing an endochitinase g...
Enhanced fungal resistance in transgenic cotton expressing an endochitinase g...
 
Folding Biologia
Folding BiologiaFolding Biologia
Folding Biologia
 
Microbiology-Phage Typing
Microbiology-Phage  TypingMicrobiology-Phage  Typing
Microbiology-Phage Typing
 
ABRCMS Poster
ABRCMS  Poster ABRCMS  Poster
ABRCMS Poster
 
ISOLATION AND DETECTION OF MUTANTS
ISOLATION AND DETECTION OF MUTANTSISOLATION AND DETECTION OF MUTANTS
ISOLATION AND DETECTION OF MUTANTS
 
Scientific Sessions 2015: Typing of STI pathogens
Scientific Sessions 2015: Typing of STI pathogensScientific Sessions 2015: Typing of STI pathogens
Scientific Sessions 2015: Typing of STI pathogens
 
KarineHerveCV2015-
KarineHerveCV2015-KarineHerveCV2015-
KarineHerveCV2015-
 
Isolation of mutant
Isolation of mutantIsolation of mutant
Isolation of mutant
 
Rosenbloom_PosterAntibiotics
Rosenbloom_PosterAntibioticsRosenbloom_PosterAntibiotics
Rosenbloom_PosterAntibiotics
 
The landscape of microbial phenotypic traits and associated genes
The landscape of microbial phenotypic traits and  associated genesThe landscape of microbial phenotypic traits and  associated genes
The landscape of microbial phenotypic traits and associated genes
 
Metagenomic
MetagenomicMetagenomic
Metagenomic
 
A windown to the past
A windown to the pastA windown to the past
A windown to the past
 
Design and Evaluation of a 16S-based Integrated Solution to Study Bacterial D...
Design and Evaluation of a 16S-based Integrated Solution to Study Bacterial D...Design and Evaluation of a 16S-based Integrated Solution to Study Bacterial D...
Design and Evaluation of a 16S-based Integrated Solution to Study Bacterial D...
 
Phage typing
Phage typingPhage typing
Phage typing
 
Isolation of antibiotic resistance mutant by replica plating
Isolation of antibiotic resistance mutant by replica platingIsolation of antibiotic resistance mutant by replica plating
Isolation of antibiotic resistance mutant by replica plating
 
Franz et al tdwg 2016 introducing lep net
Franz et al tdwg 2016 introducing lep netFranz et al tdwg 2016 introducing lep net
Franz et al tdwg 2016 introducing lep net
 
Phytobiomes
Phytobiomes Phytobiomes
Phytobiomes
 
DETECTION AND ISOLATION
DETECTION AND ISOLATIONDETECTION AND ISOLATION
DETECTION AND ISOLATION
 
Isolation of mutants
Isolation of mutantsIsolation of mutants
Isolation of mutants
 

Similar to EVOLUTION OF TUBER MELANOSPORUM MYCELIUM CONCENTRATION IN THE SOIL THROUGHOUT THE YEAR AND EVALUATION OF FUNGAL BIODIVERSITY IN PRODUCTIVE AND NONPRODUCTIVE TREES Morcillo lecture iwemm8 .compressed

Tuber borchii farming on hazels in Chile. Morcillo et al tuber 2013
Tuber borchii farming on hazels in Chile. Morcillo et al tuber 2013Tuber borchii farming on hazels in Chile. Morcillo et al tuber 2013
Tuber borchii farming on hazels in Chile. Morcillo et al tuber 2013Micologia Forestal & Aplicada
 
Vilanova et al tuber 2013 characterisation of pseuromonadaceae populations in...
Vilanova et al tuber 2013 characterisation of pseuromonadaceae populations in...Vilanova et al tuber 2013 characterisation of pseuromonadaceae populations in...
Vilanova et al tuber 2013 characterisation of pseuromonadaceae populations in...Micologia Forestal & Aplicada
 
Thrips Management Alternatives in the Field
Thrips Management Alternatives in the FieldThrips Management Alternatives in the Field
Thrips Management Alternatives in the FieldGardening
 
Benefits Over Traditional Wired Sensing Technology
Benefits Over Traditional Wired Sensing TechnologyBenefits Over Traditional Wired Sensing Technology
Benefits Over Traditional Wired Sensing TechnologyChristina Valadez
 
MAGIC population in Vegetables
MAGIC population in VegetablesMAGIC population in Vegetables
MAGIC population in VegetablesAnusha K R
 
Effect of Clariodeoglomusclariodeorum on morphology and abundant of carrot ro...
Effect of Clariodeoglomusclariodeorum on morphology and abundant of carrot ro...Effect of Clariodeoglomusclariodeorum on morphology and abundant of carrot ro...
Effect of Clariodeoglomusclariodeorum on morphology and abundant of carrot ro...AI Publications
 
Schundler UBC Polypore Proposal
Schundler UBC Polypore ProposalSchundler UBC Polypore Proposal
Schundler UBC Polypore ProposalGreg Schundler
 
Modelling Calçot Production By Means Of Gompertz Equation
Modelling Calçot Production By Means Of Gompertz EquationModelling Calçot Production By Means Of Gompertz Equation
Modelling Calçot Production By Means Of Gompertz Equationmarcalplans
 
Understanding the Rearing of mosquito in laboratory and Mosquito Vector Surve...
Understanding the Rearing of mosquito in laboratory and Mosquito Vector Surve...Understanding the Rearing of mosquito in laboratory and Mosquito Vector Surve...
Understanding the Rearing of mosquito in laboratory and Mosquito Vector Surve...Muhammad Kamran (Sial)
 
Introduction to Research Proposal
Introduction to Research ProposalIntroduction to Research Proposal
Introduction to Research ProposalJennifer Luther
 
Culture media and techniques
Culture media and techniquesCulture media and techniques
Culture media and techniquesDr. Ritu Gupta
 

Similar to EVOLUTION OF TUBER MELANOSPORUM MYCELIUM CONCENTRATION IN THE SOIL THROUGHOUT THE YEAR AND EVALUATION OF FUNGAL BIODIVERSITY IN PRODUCTIVE AND NONPRODUCTIVE TREES Morcillo lecture iwemm8 .compressed (20)

Tuber borchii farming on hazels in Chile. Morcillo et al tuber 2013
Tuber borchii farming on hazels in Chile. Morcillo et al tuber 2013Tuber borchii farming on hazels in Chile. Morcillo et al tuber 2013
Tuber borchii farming on hazels in Chile. Morcillo et al tuber 2013
 
Vilanova et al tuber 2013 characterisation of pseuromonadaceae populations in...
Vilanova et al tuber 2013 characterisation of pseuromonadaceae populations in...Vilanova et al tuber 2013 characterisation of pseuromonadaceae populations in...
Vilanova et al tuber 2013 characterisation of pseuromonadaceae populations in...
 
Thrips Management Alternatives in the Field
Thrips Management Alternatives in the FieldThrips Management Alternatives in the Field
Thrips Management Alternatives in the Field
 
Benefits Over Traditional Wired Sensing Technology
Benefits Over Traditional Wired Sensing TechnologyBenefits Over Traditional Wired Sensing Technology
Benefits Over Traditional Wired Sensing Technology
 
Research proposal
Research proposalResearch proposal
Research proposal
 
MAGIC population in Vegetables
MAGIC population in VegetablesMAGIC population in Vegetables
MAGIC population in Vegetables
 
Effect of Clariodeoglomusclariodeorum on morphology and abundant of carrot ro...
Effect of Clariodeoglomusclariodeorum on morphology and abundant of carrot ro...Effect of Clariodeoglomusclariodeorum on morphology and abundant of carrot ro...
Effect of Clariodeoglomusclariodeorum on morphology and abundant of carrot ro...
 
Unlocking the value and use potential of genetic resources
Unlocking the value and use potential of genetic resourcesUnlocking the value and use potential of genetic resources
Unlocking the value and use potential of genetic resources
 
Proposal presentation 11
Proposal presentation 11Proposal presentation 11
Proposal presentation 11
 
marais2017 (1).pdf
marais2017 (1).pdfmarais2017 (1).pdf
marais2017 (1).pdf
 
Kat.ppt
Kat.pptKat.ppt
Kat.ppt
 
Srep18078
Srep18078Srep18078
Srep18078
 
Synopsis Seminar
Synopsis Seminar Synopsis Seminar
Synopsis Seminar
 
Schundler UBC Polypore Proposal
Schundler UBC Polypore ProposalSchundler UBC Polypore Proposal
Schundler UBC Polypore Proposal
 
Modelling Calçot Production By Means Of Gompertz Equation
Modelling Calçot Production By Means Of Gompertz EquationModelling Calçot Production By Means Of Gompertz Equation
Modelling Calçot Production By Means Of Gompertz Equation
 
Magic population
Magic populationMagic population
Magic population
 
Soil microbial diversity
Soil microbial diversitySoil microbial diversity
Soil microbial diversity
 
Understanding the Rearing of mosquito in laboratory and Mosquito Vector Surve...
Understanding the Rearing of mosquito in laboratory and Mosquito Vector Surve...Understanding the Rearing of mosquito in laboratory and Mosquito Vector Surve...
Understanding the Rearing of mosquito in laboratory and Mosquito Vector Surve...
 
Introduction to Research Proposal
Introduction to Research ProposalIntroduction to Research Proposal
Introduction to Research Proposal
 
Culture media and techniques
Culture media and techniquesCulture media and techniques
Culture media and techniques
 

More from Micologia Forestal & Aplicada

Viabilidad y rentabilidad de una plantación de pino piñonero micorrizado co...
Viabilidad y rentabilidad de una plantación de pino piñonero micorrizado co...Viabilidad y rentabilidad de una plantación de pino piñonero micorrizado co...
Viabilidad y rentabilidad de una plantación de pino piñonero micorrizado co...Micologia Forestal & Aplicada
 
Xerrada efecte bacteris en la producció de tòfones Irta Micofora 2017
Xerrada efecte bacteris en la producció de tòfones Irta Micofora 2017Xerrada efecte bacteris en la producció de tòfones Irta Micofora 2017
Xerrada efecte bacteris en la producció de tòfones Irta Micofora 2017Micologia Forestal & Aplicada
 
Ponencia quantificació miceli tòfona negra al sòl i biodiversitat en tofon...
Ponencia quantificació miceli tòfona negra al sòl i biodiversitat en tofon...Ponencia quantificació miceli tòfona negra al sòl i biodiversitat en tofon...
Ponencia quantificació miceli tòfona negra al sòl i biodiversitat en tofon...Micologia Forestal & Aplicada
 
Poster tuber borchii research in portugal iwemm morocco
Poster tuber borchii research in portugal iwemm moroccoPoster tuber borchii research in portugal iwemm morocco
Poster tuber borchii research in portugal iwemm moroccoMicologia Forestal & Aplicada
 
¿Por qué es tan difícil cultivar hongos micorrícicos comestibles?
¿Por qué es tan difícil cultivar hongos micorrícicos comestibles?¿Por qué es tan difícil cultivar hongos micorrícicos comestibles?
¿Por qué es tan difícil cultivar hongos micorrícicos comestibles?Micologia Forestal & Aplicada
 
Jornada tòfones i tubericultura IRTA caldes montbui
Jornada tòfones i tubericultura IRTA caldes montbuiJornada tòfones i tubericultura IRTA caldes montbui
Jornada tòfones i tubericultura IRTA caldes montbuiMicologia Forestal & Aplicada
 
1 cultivo de setas micorrícicas comestibles 2014 marcos morcillo
1  cultivo de setas micorrícicas comestibles 2014 marcos morcillo1  cultivo de setas micorrícicas comestibles 2014 marcos morcillo
1 cultivo de setas micorrícicas comestibles 2014 marcos morcilloMicologia Forestal & Aplicada
 
2 cultivo de la trufa en el mundo 2014 marcos morcillo
2  cultivo de la trufa en el mundo 2014 marcos morcillo2  cultivo de la trufa en el mundo 2014 marcos morcillo
2 cultivo de la trufa en el mundo 2014 marcos morcilloMicologia Forestal & Aplicada
 
3 cultivo de tuber aestivum, uncinatum, borchii y brumale 2014 marcos morcillo
3  cultivo de tuber aestivum, uncinatum, borchii y brumale 2014 marcos morcillo3  cultivo de tuber aestivum, uncinatum, borchii y brumale 2014 marcos morcillo
3 cultivo de tuber aestivum, uncinatum, borchii y brumale 2014 marcos morcilloMicologia Forestal & Aplicada
 
4 mercado trufas oferta -demanda y cadena valor 2014 marcos morcillo
4  mercado trufas oferta -demanda y cadena valor 2014 marcos morcillo4  mercado trufas oferta -demanda y cadena valor 2014 marcos morcillo
4 mercado trufas oferta -demanda y cadena valor 2014 marcos morcilloMicologia Forestal & Aplicada
 
Poster morcillo et al tuber borchii micorrhization depending on host tree a...
Poster morcillo et al   tuber borchii micorrhization depending on host tree a...Poster morcillo et al   tuber borchii micorrhization depending on host tree a...
Poster morcillo et al tuber borchii micorrhization depending on host tree a...Micologia Forestal & Aplicada
 

More from Micologia Forestal & Aplicada (20)

Truffle farming guide micofora booklet
Truffle farming guide   micofora bookletTruffle farming guide   micofora booklet
Truffle farming guide micofora booklet
 
Viabilidad y rentabilidad de una plantación de pino piñonero micorrizado co...
Viabilidad y rentabilidad de una plantación de pino piñonero micorrizado co...Viabilidad y rentabilidad de una plantación de pino piñonero micorrizado co...
Viabilidad y rentabilidad de una plantación de pino piñonero micorrizado co...
 
Xerrada efecte bacteris en la producció de tòfones Irta Micofora 2017
Xerrada efecte bacteris en la producció de tòfones Irta Micofora 2017Xerrada efecte bacteris en la producció de tòfones Irta Micofora 2017
Xerrada efecte bacteris en la producció de tòfones Irta Micofora 2017
 
Ponencia quantificació miceli tòfona negra al sòl i biodiversitat en tofon...
Ponencia quantificació miceli tòfona negra al sòl i biodiversitat en tofon...Ponencia quantificació miceli tòfona negra al sòl i biodiversitat en tofon...
Ponencia quantificació miceli tòfona negra al sòl i biodiversitat en tofon...
 
Hongos silvestres en guatemala
Hongos silvestres en guatemalaHongos silvestres en guatemala
Hongos silvestres en guatemala
 
Cultivo de hongos y desarrollo rural en nicaragua
Cultivo de hongos y desarrollo rural en nicaraguaCultivo de hongos y desarrollo rural en nicaragua
Cultivo de hongos y desarrollo rural en nicaragua
 
Cómo tomar muestras de suelo
Cómo tomar muestras de sueloCómo tomar muestras de suelo
Cómo tomar muestras de suelo
 
Poster tuber borchii research in portugal iwemm morocco
Poster tuber borchii research in portugal iwemm moroccoPoster tuber borchii research in portugal iwemm morocco
Poster tuber borchii research in portugal iwemm morocco
 
¿Por qué es tan difícil cultivar hongos micorrícicos comestibles?
¿Por qué es tan difícil cultivar hongos micorrícicos comestibles?¿Por qué es tan difícil cultivar hongos micorrícicos comestibles?
¿Por qué es tan difícil cultivar hongos micorrícicos comestibles?
 
Barcelona Truffle Tour notes January 2016
Barcelona Truffle Tour notes January 2016Barcelona Truffle Tour notes January 2016
Barcelona Truffle Tour notes January 2016
 
Jornada tòfones i tubericultura IRTA caldes montbui
Jornada tòfones i tubericultura IRTA caldes montbuiJornada tòfones i tubericultura IRTA caldes montbui
Jornada tòfones i tubericultura IRTA caldes montbui
 
Cape kaliakra moments 2015 interview bulgaria
Cape kaliakra moments 2015 interview bulgariaCape kaliakra moments 2015 interview bulgaria
Cape kaliakra moments 2015 interview bulgaria
 
Barcelona truffle-tour-2015-16
Barcelona truffle-tour-2015-16Barcelona truffle-tour-2015-16
Barcelona truffle-tour-2015-16
 
Cambios de paradigma en truficultura
Cambios de paradigma en truficultura Cambios de paradigma en truficultura
Cambios de paradigma en truficultura
 
Ponencia marcos morcillo cultiu tuber borchii
Ponencia marcos morcillo   cultiu tuber borchiiPonencia marcos morcillo   cultiu tuber borchii
Ponencia marcos morcillo cultiu tuber borchii
 
1 cultivo de setas micorrícicas comestibles 2014 marcos morcillo
1  cultivo de setas micorrícicas comestibles 2014 marcos morcillo1  cultivo de setas micorrícicas comestibles 2014 marcos morcillo
1 cultivo de setas micorrícicas comestibles 2014 marcos morcillo
 
2 cultivo de la trufa en el mundo 2014 marcos morcillo
2  cultivo de la trufa en el mundo 2014 marcos morcillo2  cultivo de la trufa en el mundo 2014 marcos morcillo
2 cultivo de la trufa en el mundo 2014 marcos morcillo
 
3 cultivo de tuber aestivum, uncinatum, borchii y brumale 2014 marcos morcillo
3  cultivo de tuber aestivum, uncinatum, borchii y brumale 2014 marcos morcillo3  cultivo de tuber aestivum, uncinatum, borchii y brumale 2014 marcos morcillo
3 cultivo de tuber aestivum, uncinatum, borchii y brumale 2014 marcos morcillo
 
4 mercado trufas oferta -demanda y cadena valor 2014 marcos morcillo
4  mercado trufas oferta -demanda y cadena valor 2014 marcos morcillo4  mercado trufas oferta -demanda y cadena valor 2014 marcos morcillo
4 mercado trufas oferta -demanda y cadena valor 2014 marcos morcillo
 
Poster morcillo et al tuber borchii micorrhization depending on host tree a...
Poster morcillo et al   tuber borchii micorrhization depending on host tree a...Poster morcillo et al   tuber borchii micorrhization depending on host tree a...
Poster morcillo et al tuber borchii micorrhization depending on host tree a...
 

Recently uploaded

Triploidy ...............................pptx
Triploidy ...............................pptxTriploidy ...............................pptx
Triploidy ...............................pptxCherry
 
Exomoons & Exorings with the Habitable Worlds Observatory I: On the Detection...
Exomoons & Exorings with the Habitable Worlds Observatory I: On the Detection...Exomoons & Exorings with the Habitable Worlds Observatory I: On the Detection...
Exomoons & Exorings with the Habitable Worlds Observatory I: On the Detection...Sérgio Sacani
 
Constraints on Neutrino Natal Kicks from Black-Hole Binary VFTS 243
Constraints on Neutrino Natal Kicks from Black-Hole Binary VFTS 243Constraints on Neutrino Natal Kicks from Black-Hole Binary VFTS 243
Constraints on Neutrino Natal Kicks from Black-Hole Binary VFTS 243Sérgio Sacani
 
Film Coated Tablet and Film Coating raw materials.pdf
Film Coated Tablet and Film Coating raw materials.pdfFilm Coated Tablet and Film Coating raw materials.pdf
Film Coated Tablet and Film Coating raw materials.pdfPharmatech-rx
 
Plasma proteins_ Dr.Muralinath_Dr.c. kalyan
Plasma proteins_ Dr.Muralinath_Dr.c. kalyanPlasma proteins_ Dr.Muralinath_Dr.c. kalyan
Plasma proteins_ Dr.Muralinath_Dr.c. kalyanmuralinath2
 
Jet reorientation in central galaxies of clusters and groups: insights from V...
Jet reorientation in central galaxies of clusters and groups: insights from V...Jet reorientation in central galaxies of clusters and groups: insights from V...
Jet reorientation in central galaxies of clusters and groups: insights from V...Sérgio Sacani
 
ERTHROPOIESIS: Dr. E. Muralinath & R. Gnana Lahari
ERTHROPOIESIS: Dr. E. Muralinath & R. Gnana LahariERTHROPOIESIS: Dr. E. Muralinath & R. Gnana Lahari
ERTHROPOIESIS: Dr. E. Muralinath & R. Gnana Laharimuralinath2
 
GBSN - Biochemistry (Unit 4) Chemistry of Carbohydrates
GBSN - Biochemistry (Unit 4) Chemistry of CarbohydratesGBSN - Biochemistry (Unit 4) Chemistry of Carbohydrates
GBSN - Biochemistry (Unit 4) Chemistry of CarbohydratesAreesha Ahmad
 
The Scientific names of some important families of Industrial plants .pdf
The Scientific names of some important families of Industrial plants .pdfThe Scientific names of some important families of Industrial plants .pdf
The Scientific names of some important families of Industrial plants .pdfMohamed Said
 
WASP-69b’s Escaping Envelope Is Confined to a Tail Extending at Least 7 Rp
WASP-69b’s Escaping Envelope Is Confined to a Tail Extending at Least 7 RpWASP-69b’s Escaping Envelope Is Confined to a Tail Extending at Least 7 Rp
WASP-69b’s Escaping Envelope Is Confined to a Tail Extending at Least 7 RpSérgio Sacani
 
Land use land cover change analysis and detection of its drivers using geospa...
Land use land cover change analysis and detection of its drivers using geospa...Land use land cover change analysis and detection of its drivers using geospa...
Land use land cover change analysis and detection of its drivers using geospa...MohammedAhmed246550
 
family therapy psychotherapy types .pdf
family therapy psychotherapy types  .pdffamily therapy psychotherapy types  .pdf
family therapy psychotherapy types .pdfhaseebahmeddrama
 
The solar dynamo begins near the surface
The solar dynamo begins near the surfaceThe solar dynamo begins near the surface
The solar dynamo begins near the surfaceSérgio Sacani
 
Continuum emission from within the plunging region of black hole discs
Continuum emission from within the plunging region of black hole discsContinuum emission from within the plunging region of black hole discs
Continuum emission from within the plunging region of black hole discsSérgio Sacani
 
GBSN - Microbiology (Unit 6) Human and Microbial interaction
GBSN - Microbiology (Unit 6) Human and Microbial interactionGBSN - Microbiology (Unit 6) Human and Microbial interaction
GBSN - Microbiology (Unit 6) Human and Microbial interactionAreesha Ahmad
 
Aerodynamics. flippatterncn5tm5ttnj6nmnynyppt
Aerodynamics. flippatterncn5tm5ttnj6nmnynypptAerodynamics. flippatterncn5tm5ttnj6nmnynyppt
Aerodynamics. flippatterncn5tm5ttnj6nmnynypptsreddyrahul
 
GBSN - Microbiology (Unit 7) Microbiology in Everyday Life
GBSN - Microbiology (Unit 7) Microbiology in Everyday LifeGBSN - Microbiology (Unit 7) Microbiology in Everyday Life
GBSN - Microbiology (Unit 7) Microbiology in Everyday LifeAreesha Ahmad
 
Mining Activity and Investment Opportunity in Myanmar.pptx
Mining Activity and Investment Opportunity in Myanmar.pptxMining Activity and Investment Opportunity in Myanmar.pptx
Mining Activity and Investment Opportunity in Myanmar.pptxKyawThanTint
 
INSIGHT Partner Profile: Tampere University
INSIGHT Partner Profile: Tampere UniversityINSIGHT Partner Profile: Tampere University
INSIGHT Partner Profile: Tampere UniversitySteffi Friedrichs
 
Cell Immobilization Methods and Applications.pptx
Cell Immobilization Methods and Applications.pptxCell Immobilization Methods and Applications.pptx
Cell Immobilization Methods and Applications.pptxCherry
 

Recently uploaded (20)

Triploidy ...............................pptx
Triploidy ...............................pptxTriploidy ...............................pptx
Triploidy ...............................pptx
 
Exomoons & Exorings with the Habitable Worlds Observatory I: On the Detection...
Exomoons & Exorings with the Habitable Worlds Observatory I: On the Detection...Exomoons & Exorings with the Habitable Worlds Observatory I: On the Detection...
Exomoons & Exorings with the Habitable Worlds Observatory I: On the Detection...
 
Constraints on Neutrino Natal Kicks from Black-Hole Binary VFTS 243
Constraints on Neutrino Natal Kicks from Black-Hole Binary VFTS 243Constraints on Neutrino Natal Kicks from Black-Hole Binary VFTS 243
Constraints on Neutrino Natal Kicks from Black-Hole Binary VFTS 243
 
Film Coated Tablet and Film Coating raw materials.pdf
Film Coated Tablet and Film Coating raw materials.pdfFilm Coated Tablet and Film Coating raw materials.pdf
Film Coated Tablet and Film Coating raw materials.pdf
 
Plasma proteins_ Dr.Muralinath_Dr.c. kalyan
Plasma proteins_ Dr.Muralinath_Dr.c. kalyanPlasma proteins_ Dr.Muralinath_Dr.c. kalyan
Plasma proteins_ Dr.Muralinath_Dr.c. kalyan
 
Jet reorientation in central galaxies of clusters and groups: insights from V...
Jet reorientation in central galaxies of clusters and groups: insights from V...Jet reorientation in central galaxies of clusters and groups: insights from V...
Jet reorientation in central galaxies of clusters and groups: insights from V...
 
ERTHROPOIESIS: Dr. E. Muralinath & R. Gnana Lahari
ERTHROPOIESIS: Dr. E. Muralinath & R. Gnana LahariERTHROPOIESIS: Dr. E. Muralinath & R. Gnana Lahari
ERTHROPOIESIS: Dr. E. Muralinath & R. Gnana Lahari
 
GBSN - Biochemistry (Unit 4) Chemistry of Carbohydrates
GBSN - Biochemistry (Unit 4) Chemistry of CarbohydratesGBSN - Biochemistry (Unit 4) Chemistry of Carbohydrates
GBSN - Biochemistry (Unit 4) Chemistry of Carbohydrates
 
The Scientific names of some important families of Industrial plants .pdf
The Scientific names of some important families of Industrial plants .pdfThe Scientific names of some important families of Industrial plants .pdf
The Scientific names of some important families of Industrial plants .pdf
 
WASP-69b’s Escaping Envelope Is Confined to a Tail Extending at Least 7 Rp
WASP-69b’s Escaping Envelope Is Confined to a Tail Extending at Least 7 RpWASP-69b’s Escaping Envelope Is Confined to a Tail Extending at Least 7 Rp
WASP-69b’s Escaping Envelope Is Confined to a Tail Extending at Least 7 Rp
 
Land use land cover change analysis and detection of its drivers using geospa...
Land use land cover change analysis and detection of its drivers using geospa...Land use land cover change analysis and detection of its drivers using geospa...
Land use land cover change analysis and detection of its drivers using geospa...
 
family therapy psychotherapy types .pdf
family therapy psychotherapy types  .pdffamily therapy psychotherapy types  .pdf
family therapy psychotherapy types .pdf
 
The solar dynamo begins near the surface
The solar dynamo begins near the surfaceThe solar dynamo begins near the surface
The solar dynamo begins near the surface
 
Continuum emission from within the plunging region of black hole discs
Continuum emission from within the plunging region of black hole discsContinuum emission from within the plunging region of black hole discs
Continuum emission from within the plunging region of black hole discs
 
GBSN - Microbiology (Unit 6) Human and Microbial interaction
GBSN - Microbiology (Unit 6) Human and Microbial interactionGBSN - Microbiology (Unit 6) Human and Microbial interaction
GBSN - Microbiology (Unit 6) Human and Microbial interaction
 
Aerodynamics. flippatterncn5tm5ttnj6nmnynyppt
Aerodynamics. flippatterncn5tm5ttnj6nmnynypptAerodynamics. flippatterncn5tm5ttnj6nmnynyppt
Aerodynamics. flippatterncn5tm5ttnj6nmnynyppt
 
GBSN - Microbiology (Unit 7) Microbiology in Everyday Life
GBSN - Microbiology (Unit 7) Microbiology in Everyday LifeGBSN - Microbiology (Unit 7) Microbiology in Everyday Life
GBSN - Microbiology (Unit 7) Microbiology in Everyday Life
 
Mining Activity and Investment Opportunity in Myanmar.pptx
Mining Activity and Investment Opportunity in Myanmar.pptxMining Activity and Investment Opportunity in Myanmar.pptx
Mining Activity and Investment Opportunity in Myanmar.pptx
 
INSIGHT Partner Profile: Tampere University
INSIGHT Partner Profile: Tampere UniversityINSIGHT Partner Profile: Tampere University
INSIGHT Partner Profile: Tampere University
 
Cell Immobilization Methods and Applications.pptx
Cell Immobilization Methods and Applications.pptxCell Immobilization Methods and Applications.pptx
Cell Immobilization Methods and Applications.pptx
 

EVOLUTION OF TUBER MELANOSPORUM MYCELIUM CONCENTRATION IN THE SOIL THROUGHOUT THE YEAR AND EVALUATION OF FUNGAL BIODIVERSITY IN PRODUCTIVE AND NONPRODUCTIVE TREES Morcillo lecture iwemm8 .compressed

  • 1. EVOLUTION OF TUBER MELANOSPORUM MYCELIUM CONCENTRATION IN THE SOIL THROUGHOUT THE YEAR AND EVALUATION OF FUNGAL BIODIVERSITY IN PRODUCTIVE AND NON- PRODUCTIVE TREES Marcos Morcillo1, Javier Parladé2, Joan Pera2, Mónica Sanchez1, Xavier Vilanova1 1 MICOLOGIA FORESTAL & APLICADA. Cami Del Fou, s/n 08459 Sant Antoni De Vilamajor. (Barcelona) SPAIN 2 IRTA. Proteccio Vegetal Sostenible Ctra. de Cabrils, Km 2, 08348 Cabrils (Barcelona) SPAIN marcosmorcillo@micofora.com xavier.parlade@irta.cat
  • 2. Goals three main objectives: 1) to evaluate the seasonal dynamics of Tuber melanosporum mycelium in a plantation 2) to develop and validate a system to reinoculate black truffle in pre-productive orchards and 3) to determine fungal biodiversity in black truffle truffieres on productive and non productive trees, and reinoculated trees.
  • 4. Trial Sites Two field trials with loam soils 2013, 2014 Granollers: 11 year Q. ilex, no irrigation -  Seasonal mycelium concentration -  Metagenomic biodiversity Batea: 4 year old Q. ilex, drip irrigation -  Seasonal mycelium concentration -  Metagenomic biodiversity -  Reinoculation trials
  • 5. Quantification of soil mycelium biomass (qPCR) kit Power Soil (MoBio) 0,25 g. Soil. sondes Taqman-MGB, for T. melanosporum, Standard curves with known mycelium quantities for each trial site. Parladé et al. 2013. Mycorrhiza 23:99–106. Distance to tree (m) Concentration of mycelum mg/g * 0,2 8,090 1,5 0,008 n=6 for all treatments
  • 6. qPCR seasonal dynamics n=6 for each curve
  • 7. Evaluation of fungal diversity Metagenomic platforms n=6 for each treatment plus control. Samples mixed so a single metagenomic per treatment (no replicate)
  • 8. Soil fungal diversity 11 year-old T. melanosporum orchard
  • 9. Soil fungal diversity 11 year-old T. melanosporum orchard
  • 10. Reinoculations & spanish wells are a common practice: qPCR & biodiversity to check effectiveness
  • 11. qPCR after reinoculations Concentration of T. melanosporum mycelium in june. Control vs. Field inoculated (5g/tree) in april. Significative differences. Concentration of T. melanosporum mycelium Control vs. Field june inoculations Dose 1, 2 i 3 are 9, 5 i 2 grams of truffle /tree No significative differences.
  • 13. Conclusions •  Drip irrigation system seems not to favour truffle mycelium expansion in soil. 3 logarithms below outside humidity bubble. •  Seasonal differences in distribution of black truffle mycelium in the soil. Seasonal pattern dependent from water or irrigation. •  Significative more Tuber mycelium concentration and sequences in productive trees. •  Idem for Scleroderma. Could it be a positive partner specie? •  April inoculations increase (almost double) Tuber sp. sequences and mycelium concentration in june, as compared to controls non-inoculated, reaching same levels of sequences than when inoculated in june at 5grams. BUT for agronomic reasons it should be better to inoculate in april, as we mostly do mechanically with tractors over the brulê, in order not to disturb the active mycelium in june. •  If possible it would be interesting to do the wells with spores even in february – march so spores could have enough time to germinate and new males promote fecundations in may.
  • 14. Can these curves become reference standard curves? Future Absolute values from Real Time PCR results hardly comparable between geographic areas or when sampling and analysis are not done at the same time. But good to compare treatments from single plantations when sampled and analyzed at the same time. The results have a direct application in the development of new technologies for truffle farming: -  traceability of fungal mycelium -  methods to increase yields -  to evaluate the effects of different managements in beneficial microbial communities for the ecosystem (biodiversity). As metagenomic results seem to be quantitative, this technique may be useful for studies on diversity dynamics in a plantation, as well as to identify fungal species associated (positively or negatively) to Tuber persistence.