Expert in immunotechnology, ModiQuestResearch is a Contract ResearchOrganisation. Since its establishment in2004, the company has been theprivileged partner of prestigious industrialand academic laboratories for theirresearch, development, diagnostics andtherapeutics projects.
ModiQuest Research at a glance • LifeScience Park Oss • Company headquarters • Highly Educated & Experienced people • Contract Research Organisation • Communication • Proprietary technologies • Focus on Antibody development
Antibody generation with a strong focus on low immunogenic and difficult antigens
Monoclonal Antibodies via Hybridoma Technology Immunization • Various immunization protocols in multiple species Fusion • Proprietary electrofusion of spleen cells with myeloma cell line • Cells are seeded in semi-solid medium ClonePix-FL based cell sorting • Viability/ antibody production/ antigen binding • Individual clones are transferred to 96 well plate
Monoclonal Antibodies via B-cell selection• Proprietary antigen specific B-cell selection• Vaccinated, infectious disease, autoimmune• Rabbit, Lama, Mouse, Human
Monoclonal Antibodies via Phage Display• Human naïve scFv library• Human disease scFv libraries - e.g. Rheumatoid Arthritis, Multiple Sclerosis, Scleroderma, SLE, Malaria, Chagas• Chicken, Mouse, Rabbit scFv libraries - naïve and immune• Lama libraries - naïve and immune
Antibody ProductionMammalian antibody-expressing cell lines and vectors MQR proprietary expression vectors Fully human and chimeric antibodies: • Human IgA1, IgM, IgG1, IgG3, and IgG4 (both Kappa & Lambda) • Mouse IgG1, IgG2a, IgG2b, and IgG3 • Chicken IgY Transient production: CHO, HEK-293 Stable cell lines: CHO, HEK-293
Assay Development• Animal model: CAIA mouse model• ELISA• Flowcytometry• Bio-assay development DBA/1 mice treated with a mixture of 8 collagen- specific monoclonal antibodies (2.3 mg total). A control antibody was used at the same concentration. LPS was injected on day 7. Pictures are histological preparations of hind paws 25 days after anti-collagen injection. SO = Safranin O staining; Blue is bone, red is cartilage. HE = Haematoxylin staining; * = inflammatory infiltrate.
ModiQuest Research Workflow Antigen Antibody Antibody Antibody Antibody Assay Selection Generation Optimization Production Processing Development• Peptide Design & Modeling • Affinity maturation • Labeling• Peptide synthesis • # different proprietary vectors • Purification• # different immunization available for reformatting techniques and/or chimerization • Tagging or fusion • Humanization • Antibody sequencing via RACE • Proprietary fusion method • Many cell lines available • Functionality study • # different species & strains available • Transient production • Binding capacity study • # different techniques on different • Stable cell line • Bio-Assay species available • Gen amplification • Animal model • Polyclonal • Up to grams scale production • Hybridoma monoclonal • B-cell selection, monoclonal • Phage Display, recombinant monoclonal