Singapore grouper iridovirus (SGIV) induced
parapotosis-like cell death in host cells via the
activation of MAPK signaling...
programmed cell death , PCD
PCD is natural and genetically regulated process of removing unneeded
or damaged cells, and it...
Morphological and biochemical characteristics of apoptosis and
parapoptosis (Sperandio et al., 2000)
Virus infection induced PCD
Virus infection induced apoptosis. Virus infection induced autophagic PCD
So far, no report on...
MAPK signaling pathways and PCD
MAPKs have been identified: extracellular signal-
regulated kinase (ERK), c-Jun N-terminal...
MAPKs and virus infection
MAPK
(Han, Nat Immunol, 2006)
TLR could recognize the PAMPs of pathogens (virus, bacterial) and
...
Virus infection could activate MAPK
pathways in mammalian and humans.
Aquatic animal
viruses activate
MAPK signaling
pathw...
Iridovirus is one of the most important infectious
pathgens for maricultured grouper, Epinephelus spp !
Singapore grouper ...
1. How the SGIV interact with grouper host cells causing
cell death ?
2 、 What kind of host cell signaling pathways involv...
Huang et al, Aquaculture, 2009
SGIV infected grouper cells
Lai et al, J Fish Dis. 2008
GIV infected barramundi cells
Apopt...
Research results
1.SGIV infection induced different type of cell death and nucleus
morphology in host and non-host two fis...
Cells Characteristics
SGIV-FHM DNA fragementation, DNA ladder, TUNEL positive
SGIV-EAGS no fragementation,no DNA ladder, T...
SGIV-infectedEAGS:
appearance of cytoplamic
vaculoes, distended endoplasmic
reticulum (ER), large cluster of
swollen mitoc...
Examination of PS externalization and caspae activities
The exposure of PS on cell surface is a general marker of apoptoti...
UV-inactivated SGIV had not effect on EAGS cells, but still evoked obviously
apoptosis in FHM cells.
Virus replication was...
SGIV infection activated MAPK signaling
molecules in grouper cells
ERK, p38 MAPK and JNK molecules were phosphorylated at ...
Inhibition of ERK and JNK activities could delay the CPE and reduce the virus production.
MAPK signaling pathways were inv...
Inhibition of JNK pathway could inhibit SGIV gene transcription and
protein synthesis. No obvious changes for inhibition o...
SGIV infection evoked increased the expression of IL-8, IRF-1 and
TNFa, inhibition of JNK resulted in the reduction of 3 i...
Conclusions
SGIV infection induced nonapoptotic cell death-parapotosis
in grouper host cells.
MAPK signaling pathways involved in the SGIV
infection and replication in host cells.
ERK 、 JNK signals are essential for ...
Related publications:
1. Huang X., Huang Y., Ouyang Z., Cai J., Qin Q. 2011. J Gen Virol.,
Jun; 92:1292-301.
2. Huang X., ...
23
Acknowledges: National Basical Research
Program(973 program); NSFC; CAS
Thank you very muchThank you very much
for your attentionfor your attention !!
Upcoming SlideShare
Loading in …5
×

Singapore grouper iridovirus induced parapoptosis-like death in host cells via the activation of MAPK signaling

748 views

Published on

2013 International Symposium on Ranaviruses
by Jinlu Wu

Published in: Education, Technology
0 Comments
0 Likes
Statistics
Notes
  • Be the first to comment

  • Be the first to like this

No Downloads
Views
Total views
748
On SlideShare
0
From Embeds
0
Number of Embeds
6
Actions
Shares
0
Downloads
5
Comments
0
Likes
0
Embeds 0
No embeds

No notes for slide
  • 我们研究成果的主要创新点是 1 )。。。, 2 )。。。, 3 )。。。
  • Singapore grouper iridovirus induced parapoptosis-like death in host cells via the activation of MAPK signaling

    1. 1. Singapore grouper iridovirus (SGIV) induced parapotosis-like cell death in host cells via the activation of MAPK signalings Qiwei Qin South China Sea Institute of Oceanology Chinese Academy of Sciences 2013.7.27
    2. 2. programmed cell death , PCD PCD is natural and genetically regulated process of removing unneeded or damaged cells, and it plays critical roles in the balance between host defense and the invading pathogen during pathogen-host co-evolution. Research background (Dale E. Bredesen, 2007)
    3. 3. Morphological and biochemical characteristics of apoptosis and parapoptosis (Sperandio et al., 2000)
    4. 4. Virus infection induced PCD Virus infection induced apoptosis. Virus infection induced autophagic PCD So far, no report on virus infection induced parapoptosis !
    5. 5. MAPK signaling pathways and PCD MAPKs have been identified: extracellular signal- regulated kinase (ERK), c-Jun N-terminal kinases (JNK) and p38 MAPK ( Wada et al, 2004 )。 Mitogen-activated protein kinase (MAPK) cascades are evolutionary conserved intracellular signaling pathways that regulate gene expression, mitosis, proliferation, metabolism and programmed cell death.
    6. 6. MAPKs and virus infection MAPK (Han, Nat Immunol, 2006) TLR could recognize the PAMPs of pathogens (virus, bacterial) and trigger the host immune responses, including the activation of MAPK signaling pathways.
    7. 7. Virus infection could activate MAPK pathways in mammalian and humans. Aquatic animal viruses activate MAPK signaling pathways ??
    8. 8. Iridovirus is one of the most important infectious pathgens for maricultured grouper, Epinephelus spp ! Singapore grouper iridovirus (SGIV) isolated from diseased grouper is a novel species of ranavirus, family iridovidae ( Qin et al., 2003).
    9. 9. 1. How the SGIV interact with grouper host cells causing cell death ? 2 、 What kind of host cell signaling pathways involve in the virus infection ?
    10. 10. Huang et al, Aquaculture, 2009 SGIV infected grouper cells Lai et al, J Fish Dis. 2008 GIV infected barramundi cells Apoptosis Non-apoptosis Whether SGIV infection induced PCD is cell type dependent ?
    11. 11. Research results 1.SGIV infection induced different type of cell death and nucleus morphology in host and non-host two fish cells Cell types Characteristics SGIV-EAGS rounded, congregated, remain attach, condensed nuclei, non-apoptotic bodies SGIV-FHM rounded, detached, apoptotic bodies observed
    12. 12. Cells Characteristics SGIV-FHM DNA fragementation, DNA ladder, TUNEL positive SGIV-EAGS no fragementation,no DNA ladder, TUNEL negetive DNA fragmentation and DAN content analysis of SGIV-infected two fish cells
    13. 13. SGIV-infectedEAGS: appearance of cytoplamic vaculoes, distended endoplasmic reticulum (ER), large cluster of swollen mitochondria. SGIV-FHM: ER swelling
    14. 14. Examination of PS externalization and caspae activities The exposure of PS on cell surface is a general marker of apoptotic cells. The exposure of PS only occurred on the FHM cell surface during SGIV infection. Caspase-3 and -9 were activated in SGIV-infected FHM cells, but not in infected EAGS cells.
    15. 15. UV-inactivated SGIV had not effect on EAGS cells, but still evoked obviously apoptosis in FHM cells. Virus replication was essential for SGIV induced nonapoptotic cell death, but not for apoptosis.
    16. 16. SGIV infection activated MAPK signaling molecules in grouper cells ERK, p38 MAPK and JNK molecules were phosphorylated at the different stages during SGIV infection in host EAGS cells.
    17. 17. Inhibition of ERK and JNK activities could delay the CPE and reduce the virus production. MAPK signaling pathways were involved in SGIV replication in grouper host cells
    18. 18. Inhibition of JNK pathway could inhibit SGIV gene transcription and protein synthesis. No obvious changes for inhibition of p38 MAPK. JNK but not p38 MAPK is important for SGIV transcription and protein synthesis
    19. 19. SGIV infection evoked increased the expression of IL-8, IRF-1 and TNFa, inhibition of JNK resulted in the reduction of 3 immune genes, inhibition of p38 MAPK only reduced the expression of Fish immune genes were regulated by MAPKs IL-8 IRF-1 TNF-a
    20. 20. Conclusions SGIV infection induced nonapoptotic cell death-parapotosis in grouper host cells.
    21. 21. MAPK signaling pathways involved in the SGIV infection and replication in host cells. ERK 、 JNK signals are essential for virus replication and virus induced parapotosis SGIV-activated MAPK pathways could modulate the expression of immune genes and inflammatory cytokines
    22. 22. Related publications: 1. Huang X., Huang Y., Ouyang Z., Cai J., Qin Q. 2011. J Gen Virol., Jun; 92:1292-301. 2. Huang X., Huang Y., Ouyang Z., Xu L, Yan Y, Cui H, Han X, Qin Q. 2011. Apoptosis, Aug;16(8):831-45.
    23. 23. 23 Acknowledges: National Basical Research Program(973 program); NSFC; CAS
    24. 24. Thank you very muchThank you very much for your attentionfor your attention !!

    ×