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Blood smear evaluation

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Blood smear evaluation

  1. 1. BLOOD SMEAR EVAL
  2. 2. FEATHERED EDGE Quick scan on low power through entire slide for adequate distribution of cells Check for plt clumping & atypical cells at feathered edge. (if there is plt clumping there is no need to try and count plt in the monolayer)
  3. 3. MONOLAYER Where the action is at: diff 40X morph 100X  WBC morph, RBC morph, plt count (if no plt clumping at feathered edge)
  4. 4. NORMAL RBCS Canine-Biconcave with area of central pallor 7um Feline-no consistent central pallor, vary more in shape 6um
  5. 5. RBC MORPHOLOGY Evidence of regeneration  Polychromatophils-anisocytosis  nRBC (<4/100WBCs)
  6. 6. AUTOAGGLUTINATION
  7. 7. SPHEROCYTES Spherical, Loss normal bioconcave shape, no central pallor >5/HPF is abnormal
  8. 8. CRENATION/ECCHINOCYTES Often shrinking artifact-abn lytes, uremia, rattlesnake envenoation.
  9. 9. ACANTHOCYTES Irregularly spaced membrane projections-abnormal accumulation of lipids in RBC membrane-metabolic, HAS, dz of liver, spleen and kidney
  10. 10. SCHISTOCYTE Fragmented by mechanical injury. DIC, microvascular hemolysis, HAS, heartworm dz. 1/ 3HPF is abnormal
  11. 11. HEINZ BODIES Accumulations of damaged Hg-acetaminophen toxicosis, acute onion and zinc toxicity in dogs, DM, lymphoma in cats
  12. 12. BASOPHILIC STIPPLING Punctate aggregates of ribosomes-from highly regenerative anemia or lead toxicosis
  13. 13. HOWELL-JOLLY BODIES Nuclear remnants-regenerative anemia or splenic disease
  14. 14. PLATELETS # per 10HPF X 15,000
  15. 15. WBC MORPHOLOGY Neutrophils-lobulated nucleus with pale to light pink/ purple cytoplasm
  16. 16. BAND NEUTROPHIL U or J shaped nucleus, constriction of band <1/2 width of remainder, cytoplasm similar to mature neutrophils. <5% normal.
  17. 17. TOXIC CHANGES Presence of dohle bodies, cytoplasmic basophilia (cytoplasm is more blud- purple), foamy cytoplasmic vacuolization (soap bubbles).
  18. 18. LYMPHOCYTES Densely staining round to oval nuclei with small amounts of cytoplasm. Reactive (antigenic stimulation) darker cytoplasm, increased size.
  19. 19. MONOCYTES Lots of light to deep blue cytoplasm (darker then neut) with nuclei that is not round. Quite variable in appearance. Often larger then neuts and lymphs.
  20. 20. EOSINOPHILS In low numbers, slightly smaller then neuts with less lobulated nuclei, contains prominent pink granules which are rod shaped in cats.
  21. 21. BASOPHILS Largest, very uncommon, nucleus is ribbon like (not segmented) often with pink/ purple granules present.

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