Cells: Honors Biology ~ Edgar
 
 
 
 
 
 
 
 
Leukocyte Extravasation   <ul><li>Chemoattraction </li></ul><ul><ul><li>Cytokines </li></ul></ul><ul><ul><li>Cellular adhe...
Cytokine: induce directed chemotaxis in nearby responsive cells
 
Harvard BioVisions
Cell Biology and Diabetes
Insulin
 
 
 
 
Cells in the pancreas of a 1-week-old mouse that express the Ptf1a transcription factor gene are revealed in this photogra...
 
 
Red arrows indicate Beta Cells
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Proinsulin - Orange
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Igf-1
Insulin-like growth factor 1
 
 
 
Looking good.  The pancreas of a mouse after it was transplanted with human beta cells ( left ) looks similar to that of a...
Pulse Chase Experiment
 
 
 
 
 
 
 
 
 
mRNA Protein Localization <ul><li>Some cells make 10,000+ proteins. </li></ul><ul><li>Typical Mammalian cell will contain ...
 
Cell Membranes and Transport Honors Biology ~ Edgar
 
 
 
 
 
Jmol
Harvard BioVisions
 
 
 
 
 
 
Sodium Potassium Pump
 
 
 
 
 
Gramicidin <ul><li>Antibiotic for gram positive bacteria. </li></ul><ul><li>Limited to topical application. </li></ul><ul>...
Prediction: ion flow higher, the same or lower?
 
 
 
 
 
 
 
Passive Transport
 
 
Osmosis
 
 
 
 
Osmosis
 
Concept Check <ul><li>If a Paramecium were to swim from a hypotonic environment to an isotonic one, would the activity of ...
 
Concept Check <ul><li>This diagram represents osmosis of water across a semipermeable membrane. The U-tube on the right sh...
Answer <ul><li>This diagram represents osmosis of water across a semipermeable membrane. The U-tube on the right shows the...
 
 
Upcoming SlideShare
Loading in …5
×

Honors - Cells & insulin, membrane and transport 1112b

457 views

Published on

Published in: Technology, Education
  • Be the first to comment

  • Be the first to like this

Honors - Cells & insulin, membrane and transport 1112b

  1. 1. Cells: Honors Biology ~ Edgar
  2. 10. Leukocyte Extravasation <ul><li>Chemoattraction </li></ul><ul><ul><li>Cytokines </li></ul></ul><ul><ul><li>Cellular adhesion molecules on endothelial cells </li></ul></ul><ul><li>Rolling Adhesion </li></ul><ul><ul><li>Selectin ~ carbohydrate ligands </li></ul></ul><ul><li>Tight Adhesion </li></ul><ul><ul><li>Juxtacrine signaling </li></ul></ul><ul><ul><li>Immobilize leucocytes </li></ul></ul><ul><li>Transmigtation </li></ul><ul><ul><li>chemotactic gradient </li></ul></ul>
  3. 11. Cytokine: induce directed chemotaxis in nearby responsive cells
  4. 13. Harvard BioVisions
  5. 14. Cell Biology and Diabetes
  6. 15. Insulin
  7. 20. Cells in the pancreas of a 1-week-old mouse that express the Ptf1a transcription factor gene are revealed in this photograph. The Ptf1a gene has been genetically engineered to express a bacterial enzyme that produces a dark blue color. In both mice and humans, Ptf1a is essential for formation of the entire pancreas, including insulin-secreting beta cells. By tracing the “cell lineage,” or family history, of Ptf1a-expressing cells, scientists hope to learn more about how to maintain—or restore—the function of beta cells. At top right is the sausage-shaped spleen (light orange), and at bottom is the duodenum. Photo by Fong Cheng Pan, Ph.D., research fellow, Department of Cell & Developmental Biology, Vanderbilt University. Courtesy of Christopher V. E. Wright, D.Phil.
  8. 23. Red arrows indicate Beta Cells
  9. 42. Proinsulin - Orange
  10. 57. Igf-1
  11. 58. Insulin-like growth factor 1
  12. 62. Looking good. The pancreas of a mouse after it was transplanted with human beta cells ( left ) looks similar to that of an animal that produces insulin normally ( right ). CREDIT: Narushima et al., Nature Biotechnology Brimming with b's. Newfound cells in the pancreas give rise to neurons (red) and insulin-producing b cells (green). CREDIT: SEABERG ET AL. , NATURE BIOTECHNOLOGY The full picture. Human ES cells can eventually give rise to cells that resemble pancreatic beta cells (labeled β).
  13. 63. Pulse Chase Experiment
  14. 73. mRNA Protein Localization <ul><li>Some cells make 10,000+ proteins. </li></ul><ul><li>Typical Mammalian cell will contain a billion protein molecules </li></ul><ul><li>How do cells get their proteins to their correct destinations and keep those molecules out of the wrong places? </li></ul>
  15. 75. Cell Membranes and Transport Honors Biology ~ Edgar
  16. 81. Jmol
  17. 82. Harvard BioVisions
  18. 89. Sodium Potassium Pump
  19. 95. Gramicidin <ul><li>Antibiotic for gram positive bacteria. </li></ul><ul><li>Limited to topical application. </li></ul><ul><li>Induces hemolysis at lower concentration then bacteria ….therefore no internal use. </li></ul>
  20. 96. Prediction: ion flow higher, the same or lower?
  21. 104. Passive Transport
  22. 107. Osmosis
  23. 112. Osmosis
  24. 114. Concept Check <ul><li>If a Paramecium were to swim from a hypotonic environment to an isotonic one, would the activity of its contractile vacuole increase or decrease? Why? </li></ul>
  25. 116. Concept Check <ul><li>This diagram represents osmosis of water across a semipermeable membrane. The U-tube on the right shows the results of the osmosis. What could you do to level the solutions in the two sides of the right hand U-tube? </li></ul><ul><ul><li>Add more water to the left hand side. </li></ul></ul><ul><ul><li>Add more water to the right hand side. </li></ul></ul><ul><ul><li>Add more solute to the left hand side. </li></ul></ul><ul><ul><li>Add more solute to the right hand side. </li></ul></ul>0
  26. 117. Answer <ul><li>This diagram represents osmosis of water across a semipermeable membrane. The U-tube on the right shows the results of the osmosis. What could you do to level the solutions in the two sides of the right hand U-tube? </li></ul><ul><ul><li>c) Add more solute to the left hand side. </li></ul></ul>0

×