The post-genomic era: epigenetic sequencing applications and data integration
by dr. ir. Maté Ongenaert - Center for Medical Genetics, Ghent University
The past decade is known as the post-genomic era. Ever since the first published human genomes, the pace to determine new genomes ever increased. In addition, a number of new sequencing applications gave access to previously unexplored areas at a genome-wide scale such as whole epigenomes.
In this talk, the data generated from a number of sequencing techniques to determine whole DNA-methylomes and whole genome histone marks will be discussed.
Main goal: to convince scientists that the analysis tools have matured to a level that, using a good manual and insight in the mechanisms behind the analysis, they can do their own basic analyses.
Starting from a raw sequence file, over quality control to mapping to the reference genome, peak calling, visualization and identification of differentially methylated sites: within the time-frame of this talk, the entire process will be demonstrated.
As epigenetics regulates genomic processes and literally is a layer above genetics, able to fine-tune regulatory processes, several layers of information should be look at to understand the underlying mechanisms.
Important aspect in the analysis of epigenetic datasets thus is the integration of several data sources (expression results, re-expression results, DNA-methylation information and histone-modifications).