Successfully reported this slideshow.
We use your LinkedIn profile and activity data to personalize ads and to show you more relevant ads. You can change your ad preferences anytime.

The Role of Vitamin D in Breast Cancer

5,972 views

Published on

The effects of radiation on vitamin D actions in normal mammary cells, by Natalia L. Martinez

Published in: Health & Medicine
  • Dating direct: ♥♥♥ http://bit.ly/2F7hN3u ♥♥♥
       Reply 
    Are you sure you want to  Yes  No
    Your message goes here
  • Follow the link, new dating source: ♥♥♥ http://bit.ly/2F7hN3u ♥♥♥
       Reply 
    Are you sure you want to  Yes  No
    Your message goes here
  • hundreds of girls living near you. F.U.C.K ONE TONIGHT! ➤➤ http://t.cn/AiuWKDWR
       Reply 
    Are you sure you want to  Yes  No
    Your message goes here

The Role of Vitamin D in Breast Cancer

  1. 1. The Role of Vitamin D in Breast Cancer : The effects of radiation on vitamin D actions in normal mammary cells Department of Chemistry, The College of New Rochelle Natalia L. Martinez Mentor: Elvira Longordo University at Albany Summer Research Program GEN*NY*Sis Center for Excellence in Cancer Genomics JoEllen Welsh and Erika LaPorta
  2. 2. Breast Cancer • Breast Cancer is the uncontrolled growth of breast cells, in the lobules or the ducts Normal Cancer
  3. 3. Cellular Origin of Cancer
  4. 4. What Causes Cancer? Intrinsic Factors • Heredity • Diet • Hormones Extrinsic Factors • Chemicals • Radiation
  5. 5. DNA Damage • Chemicals and Radiation can damage DNA • Cells can sense and repair damage – p53- acts in the damage response mechanism – tumor suppressor • There is now a focus on identifying additional agents that enhance DNA repair •http://www.haemophilia.org.nz/DBimages/FCK/Image/dna.jpg
  6. 6. The link between Cancer & vitamin D • Recent research has shown a link between vitamin D and the prevention and treatment of Breast Cancer • Role of Vitamin D –Regulates Calcium and Phosphorus levels in the blood –Prevention of Rickets
  7. 7. How is Vitamin D obtained? • Food Sources – Fatty Fish – Eggs – Fortified Foods • Sunlight Exposure – UVB light
  8. 8. From Vitamin D to 1α,25(OH)2D3 7-dehydrocholesterol found in the skin cholecalciferol Or vitamin D3. forming 25- hydroxycholecalciferolProducing the active form of vitamin D3, 1,25-dihydroxycholecalciferol.
  9. 9. UV Stimulated Synthesis of Calcitriol Biochemistry Fifth Ed. Berg, Tymoczo and Stryer. W.H. Freeman, 2002.
  10. 10. The vitamin D receptor (VDR) • It is found in the nucleus of different tissues through the body • VDR is a nuclear hormone receptor • VDR is the major mediator of 1,25D actions
  11. 11. 1,25D & VDR When the active form of vitamin D binds to its nuclear receptor VDR it regulates gene expression which influences cellular proliferation, differentiation and apoptosis
  12. 12. In My Project: • Study the effects of radiation on vitamin D actions in normal human mammary cells (HME) Hypothesis: • DNA damage caused by ionizing radiation will up- regulate p53 and VDR expression Western Blot • Protection of the cells can be achieved by the ligand binding of 1,25D3 to VDR in damaged cells Crystal violet assay & immunoflurescent microscopy
  13. 13. Investigated the effects of radiation on vitamin D receptor (VDR) expression
  14. 14. Procedure • Cell Culture -HME cells (9 plates) • Ionizing Radiation DOSES OF EXPOSURE Incubation Period. Sham-0KV-0mA; 1min. 3 plates –analyzed after 1hr. 2Gy- 130KV- 2mA; 1min. 3 plates- analyzed after 3hr. 8Gy- 130KV- 7.7mA; 1 min 3 plates- analyzed after 6hr. http://www.instapstudycenter.net/images/faxitron.GIF
  15. 15. Detection of p53 and VDR by Western Blot Lamin A/C Lamin A/C 8Gy 2Gy Sham. 8Gy. 2Gy Sham Sham 2Gy 8Gy p53 VDR 6hrs. 3hrs. 1hr.
  16. 16. Use immunofluorescence microscopy to monitor expression and sub-cellular localization of VDR after radiation exposure in the presence and absence of 1,25D Detection of VDR by Confocal Microscopy www.ion.ucl.ac.uk/.../neuropath/techniques.htm
  17. 17. Results 1,25DEtOH
  18. 18. Results – 1hr
  19. 19. Results – 3hr
  20. 20. Examine whether treatment with 1,25D alters HME cell response to radiation.
  21. 21. Procedure • Plated cells in 6 well plates • Treated with 100nM Vitamin D – 48hr. Prior to radiation exposure – Just before radiation exposure • Cultured for 4 days • Quantified cell density using Crystal Violet Assay
  22. 22. Morphology Sham-EtOH Sham-VitD 2Gy-EtOH 2Gy-VitD
  23. 23. Results Pretreated EtOH Sham EtOH 2Gy VitD Sham VitD 2Gy 0 1 2 3 * * Treatment Absorbance590 Cotreated EtOH Sham EtOH 2Gy VitD Sham VitD 2Gy 0 1 2 3 * * Treatment Absorbance590
  24. 24. Conclusion • HME cells express VDR & p53 proteins • Radiation may increase VDR & p53 expression • VDR localization is altered by radiation treatment • Vitamin D inhibits growth of HME cells
  25. 25. Acknowledgments • Dr. JoEllen Welsh • Erika LaPorta • Dr. Meggan Keith • Dr. Judy Narvaez • Dr. Christopher Fernando • Deanna Morehead • UASRP Staff and Scholars

×