M. Boonmee , S. Intarapanich, O. Cotano Department of Biotechnology Faculty of Technology, Khon Kaen University
Cane sugar sugar Chemicals Ethanol Ethyl acetate Acetic acid
Strain selection (TISTR) Batch fermentation Fed-batch fermentation Initial ethanol concentration Productivity (flask) Aera...
46.0 44.3 42.6 19 ± 2 °C 23 ± 2 °C 26 ± 2 °C 38 h 40 h Acetic acid production at room temperature (flask shaking at 200 rpm)
Effect of controlled temperature (acetic acid concentration) 44.6 44.4 46.0 44.3 42.6 44.9
Effect of controlled temperature (acetic acid yield) 1.02 0.97 0.94 0.97 0.93 0.92
Effect of controlled temperature (acetic acid productivity) 0.59 0.86 0.57 0.70 0.35 0.87
Acetic acid production  in 2–L fermenter (2 vvm, pO 2  20%  ,  30°C) 19.7
Acetic acid production  in 2–L fermenter (2 vvm, pO 2  20%  ,  30°C) 18.8
Acetic acid production  in 2–L fermenter (2 vvm, pO 2  20%  ,  30°C) 20.7
<ul><li>Temperature variation effects acid productivity, not final concentration </li></ul><ul><li>A. pasteurianus  tolera...
<ul><li>The Office of the Cane and Sugar Board </li></ul><ul><li>Department of Biotechnology, KKU </li></ul>
M. Boonmee , S. Intarapanich, O. Cotano Department of Biotechnology Faculty of Technology, Khon Kaen University
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Effect of Temperature Control on Acetic Acid Production

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Presentation given at Naraesuan University Conference in 2007

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Effect of Temperature Control on Acetic Acid Production

  1. 1. M. Boonmee , S. Intarapanich, O. Cotano Department of Biotechnology Faculty of Technology, Khon Kaen University
  2. 2. Cane sugar sugar Chemicals Ethanol Ethyl acetate Acetic acid
  3. 3. Strain selection (TISTR) Batch fermentation Fed-batch fermentation Initial ethanol concentration Productivity (flask) Aeration study pH study
  4. 4. 46.0 44.3 42.6 19 ± 2 °C 23 ± 2 °C 26 ± 2 °C 38 h 40 h Acetic acid production at room temperature (flask shaking at 200 rpm)
  5. 5. Effect of controlled temperature (acetic acid concentration) 44.6 44.4 46.0 44.3 42.6 44.9
  6. 6. Effect of controlled temperature (acetic acid yield) 1.02 0.97 0.94 0.97 0.93 0.92
  7. 7. Effect of controlled temperature (acetic acid productivity) 0.59 0.86 0.57 0.70 0.35 0.87
  8. 8. Acetic acid production in 2–L fermenter (2 vvm, pO 2 20% , 30°C) 19.7
  9. 9. Acetic acid production in 2–L fermenter (2 vvm, pO 2 20% , 30°C) 18.8
  10. 10. Acetic acid production in 2–L fermenter (2 vvm, pO 2 20% , 30°C) 20.7
  11. 11. <ul><li>Temperature variation effects acid productivity, not final concentration </li></ul><ul><li>A. pasteurianus tolerates lower temperature than A. aceti. </li></ul><ul><li>Aeration in fermentor allows better productivity with the expense of substrate loss. </li></ul>
  12. 12. <ul><li>The Office of the Cane and Sugar Board </li></ul><ul><li>Department of Biotechnology, KKU </li></ul>
  13. 13.
  14. 14. M. Boonmee , S. Intarapanich, O. Cotano Department of Biotechnology Faculty of Technology, Khon Kaen University

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