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tissue culturing of sugercane

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tissue culturing

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tissue culturing of sugercane

  1. 1. Micropropogation of saccharum (sugarcane) By: Maira sultan
  2. 2. Introduction • Grass family (Poaceae) • Main product = sucrose fig. 1: sugarcane feilds • World's largest crop by production quantity • Brazil =largest producer • Pakistan =Punjab = 60 - 65 %
  3. 3. Tissue culture needs • Rapid, large-scale production • Disease-free • Newly developed varieties • Speed up the breeding • Commercialization process
  4. 4. Tissue culture Protocols 1. Explant source 2. Medium and condition 3. Callus induction 4. Shoot regeneration 5. Rooting medium 6. Environmental condition fig.2: invitro cultures 7. Acclimatization
  5. 5. Explant source • Healthy young meristems • Removing leaf sheath • Cut into 1.0 -1.5 cm length • Washed for 20-30 minutes • Alcohol (70 %) for 30 seconds fig.3: young sugarcane plants
  6. 6. medium and conditions • Semisolid basal MS medium • Concentrations and combinations of different PGRs
  7. 7. Callus induction • range of concentration of auxins • 2,4-D (0.5,1.0,2.0,2.5,3.0 and 4.0 mg/l) • IBA (0.5,1.0,2.0,2.5,3.0 and 4.0 mg/l) • NAA (0.5,1.0,2.0,2.5,3.0 and 4.0 mg/l) Fig.4: callus formation in sugarcane
  8. 8. Shoot regeneration • White friable calli • Cultured on MS medium • Combinations and concentrations of: BAP, Kinetin (0.5- 4.0mg/l) IBA, NAA (0.1-1.0mg/l) • Multiple shoot regeneration individually or in combination
  9. 9. Rooting • Half-strength (1/2 MS) • IBA • NAA • IAA (0.5-3.0mg/l) fig.5: roots of sugarcane
  10. 10. Environmental conditions • pH = 5.8 before gelling • Photoperiod = 16h • Temperature = 25 ± 3 C • Relative humidity = 70-80%
  11. 11. Acclimatization • Culture medium • plastic trays • 1/8 MS basal salt solution • After 30 days transplanted in to field condition
  12. 12. Results • Callus Induction: • Regeneration of Micro shoots:
  13. 13. Thank you

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