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SPLCV, Whiteflies & MeSummer 2011<br />August  6, 2011<br />Laura M. Jenkins<br />U.S. Vegetable Laboratory<br />2700 Sava...
Sweet Potatoes & SPLCV<br />Sweet Potato Leaf Curl Virus (SPLCV) Transmission<br />Purpose: To determine if SPLCV could be...
Grafted I. Setosaonto uninfected sweet potato plants</li></li></ul><li>Sweet Potatoes & SPLCV<br />Sweet Potato Leaf Curl ...
Grafted uninfected sweet potato plants onto I. Setosa</li></li></ul><li>Sweet Potatoes & SPLCV<br />Sweet Potato Leaf Curl...
Whitefly Cultivation<br />Whitefly Cultivation Study<br /><ul><li>Purpose: To evaluate population dynamics of whiteflies b...
Cucumbers: Flowered & Non-Flowered
Squash: Flowered & Non-Flowered</li></li></ul><li>Whitefly Cultivation<br />Whitefly Cultivation Study<br /><ul><li>Purpos...
2:1 ratio for non-flowered cucumber to flowered cucumber leaves respectively</li></li></ul><li>DNA Mutations<br /><ul><li>...
Purpose: To determine if diploidic watermelons can be mutated into tetraploidic watermelons and yield advantageous charact...
Procedures for DNA Extraction & Analysis:
Collected tissue samples from potentially mutated watermelon leaves
Utilized enzymes, buffers, sand, ceramic beads, centrifuging, vortexing and incubation to separate the supernatant from ex...
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SPLCV & Whiteflies

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Summer Internship Research at the USDA Vegetable Laboratory

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SPLCV & Whiteflies

  1. 1. SPLCV, Whiteflies & MeSummer 2011<br />August 6, 2011<br />Laura M. Jenkins<br />U.S. Vegetable Laboratory<br />2700 Savannah Highway <br />Charleston, SC<br />
  2. 2. Sweet Potatoes & SPLCV<br />Sweet Potato Leaf Curl Virus (SPLCV) Transmission<br />Purpose: To determine if SPLCV could be transferred from one SPLCV host (Ipomoea Setosa) to other potential hosts (I. Pes-trigridis, I. Tricolor, etc.)<br />Procedures:<br /><ul><li>Utilized collard greens (Brassicaoleracea L.), a non-host, to create an environment for the sweet-potato crop-eating whitefly (Bemisiatabaci) population to thrive
  3. 3. Grafted I. Setosaonto uninfected sweet potato plants</li></li></ul><li>Sweet Potatoes & SPLCV<br />Sweet Potato Leaf Curl Virus (SPLCV) Transmission<br />Purpose: To determine if SPLCV could be transferred from one SPLCV host (Ipomoea Setosa) to other potential hosts (I. Pes-trigridis, I. Tricolor, etc.)<br />Procedures:<br /><ul><li>Utilized collard greens (Brassicaoleracea L.), a non-host, to create an environment for the whitefly (Bemisiatabaci) population to thrive
  4. 4. Grafted uninfected sweet potato plants onto I. Setosa</li></li></ul><li>Sweet Potatoes & SPLCV<br />Sweet Potato Leaf Curl Virus (SPLCV) Transmission<br />Purpose: To determine if SPLCV could be transferred from one SPLCV host (Ipomoea Setosa) to other potential hosts (I. Pes-trigridis, I. Tricolor, etc.)<br />Conclusions: PCR and grafting techniques still underway<br />
  5. 5. Whitefly Cultivation<br />Whitefly Cultivation Study<br /><ul><li>Purpose: To evaluate population dynamics of whiteflies by creating the ideal environment</li></ul>Procedures:<br /><ul><li>Count whiteflies and eggs present on various plants:
  6. 6. Cucumbers: Flowered & Non-Flowered
  7. 7. Squash: Flowered & Non-Flowered</li></li></ul><li>Whitefly Cultivation<br />Whitefly Cultivation Study<br /><ul><li>Purpose: To evaluate population dynamics of whiteflies by creating the ideal environment</li></ul>Preliminary Egg Laying Conclusions:<br /><ul><li>No real preference between squash flowered or non-flowered leaves (50-50 split)
  8. 8. 2:1 ratio for non-flowered cucumber to flowered cucumber leaves respectively</li></li></ul><li>DNA Mutations<br /><ul><li>DNA Mutation of Diploidic Watermelons
  9. 9. Purpose: To determine if diploidic watermelons can be mutated into tetraploidic watermelons and yield advantageous characteristics (ex. seedless)
  10. 10. Procedures for DNA Extraction & Analysis:
  11. 11. Collected tissue samples from potentially mutated watermelon leaves
  12. 12. Utilized enzymes, buffers, sand, ceramic beads, centrifuging, vortexing and incubation to separate the supernatant from excess debris and reveal the DNA fragments
  13. 13. Created stock solutions to bring all DNA samples to the same concentration</li></li></ul><li>DNA Mutations<br /><ul><li>DNA Mutation of Diploidic Watermelons
  14. 14. Purpose: To determine if diploidic watermelons can be mutated into tetraploidic watermelons and yield advantageous characteristics (ex. seedless)
  15. 15. Procedures for DNA Extraction & Analysis:
  16. 16. Polymerase chain reaction (PCR) technique used to reveal the desired mutation in the DNA sequence
  17. 17. Fragment Analysis software revealed similar size characteristics for various watermelon components (nucleotides, etc.) using dye primers and a standard</li></li></ul><li>DNA Mutations<br />DNA Mutation of Diploidic Watermelons<br />Purpose: To determine if diploidic watermelons can be mutated into tetraploidic watermelons and yield advantageous characteristics (ex. seedless)<br />Conclusions: Self-Pollination techniques were unsuccesful; Natural pollination (bee pollination) currently underway<br />
  18. 18. A Bug’s Life<br />Beetle Sustainability Study<br /><ul><li>Purpose: To evaluate population dynamics of beetles under various environments</li></ul>Procedures:<br /><ul><li>Extract the pupae of several beetles from underneath collard green leaves
  19. 19. Create environments that reveal beetle survival rates under various nutritional parameters: Honeydew, Whitefly, Honey & Water</li></li></ul><li>A Bug’s Life<br />Beetle Sustainability Study<br /><ul><li>Purpose: To evaluate population dynamics of beetles under various environments</li></ul>Conclusions: Whitefly Habitat > Honey > Honeydew > Water<br />
  20. 20. Acknowledgements<br />I would like to thank the following people for their excellent insight and superb technical expertise:<br /> Dr. Alvin Simmons<br /> Dr. Amnon Levi<br /> Bradford Peck<br /> Laura Pence<br /> Paul Wade<br />

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