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  1. 1. Objective• Withering syndrome is a fatal disease of abalone caused by a rickettsial bacterium (WS-RLO)• WS-RLO transmission experiments demonstrate progeny from disease selected populations are more resistant to WS-RLO than naïve populations• GOAL: Characterize and compare the transcriptomes of WS-resistant and naïve abalone to elucidate mechanisms associated with increased disease tolerance
  2. 2. Approach1. WS-Resistant 2. WS-Resistant 3. Naïve 4. Naïve Exposed Control Exposed Control De novo assemble & annotate RNA-Seq & DESeq Galaxy – join annotation & GO Enrichment Analysis - DAVID Visualize data - REVIGO
  3. 3. Discovery: QC Losing large # of reads after trimming – trim on length only Working – RNA-Seq using trimmed and untrimmed files Assessing different RNA-Seq software – CLC vs Tophat Working – Annotating 3 assemblies (blastx)
  4. 4. Discovery(enrichment only on 2naïve libraries) EXPOSEDLess DEG in naïve WS-RLO exposed animalsShift from homeostaticprocesses to catabolicprocesses(autophagy, cytolysis, antioxidants)Consistent withcharacteristics ofwithering syndrome so CONTROLpretty cool Next StepPick “best” assembly &RNA-Seq for DESeq andenrichment analysis onall 4 libraries