Successfully reported this slideshow.
We use your LinkedIn profile and activity data to personalize ads and to show you more relevant ads. You can change your ad preferences anytime.

recombinant DNA techolongy

a basic introduction to Genetic engineering, its applications & Genetically modified food

  • Login to see the comments

recombinant DNA techolongy

  1. 1. Genetic Engineering & GMF Sami 05/26/09
  2. 2. Content 05/26/09 <ul><li>Introduction to Genetic Engineering </li></ul><ul><li>Process of GE </li></ul><ul><li>Applications of GE </li></ul><ul><li>Genetically Modified Crop </li></ul><ul><li>Benefits of GMC </li></ul><ul><li>Risks of GMC </li></ul><ul><li>Labelling of GMC </li></ul>
  3. 3. Genetic Engineering 05/26/09
  4. 4. 05/26/09 <ul><li>Transgenes are inserted by using vectors like : </li></ul><ul><ul><li>Bacterial Plasmids </li></ul></ul><ul><ul><li>Biolistic </li></ul></ul><ul><ul><li>Viruses </li></ul></ul>
  5. 5. Restriction Enzymes & Plasmids 05/26/09
  6. 6. Gene gun 05/26/09 In Biolistic usage, a microscopic gold beads coated with a transgene & shot into the cell with a pulse of helium. Inside, transgene comes off the bead and integrates into the cell's genome through transcription
  7. 7. Medical Application <ul><ul><li>Production of pharmaceuticals: </li></ul></ul><ul><ul><ul><li>Treatment of diseases (human insulin & antibiotics). </li></ul></ul></ul><ul><ul><ul><li>Disease prevention. (hepatitis B vaccine) </li></ul></ul></ul><ul><li>Clonal propagation: a source of tissue or organ for transplantation avoid all problems of immunoin-compatibility </li></ul><ul><li>Gene therapy: Artificially replace the disease-causing gene with a normal allele. By using a virus vector to the target tissues. </li></ul>05/26/09
  8. 8. Industrial Applications <ul><li>Use of GM MO to make stone-wash jeans & to produce detergents. </li></ul><ul><li>GM MO with enhanced ability to break down environmental pollutants (E. coli to break down cellulose) </li></ul><ul><li>Fast growing Salmon </li></ul>05/26/09 Tomato cell Fish cell Fish DNA Denucleated fish egg
  9. 9. Agricultural Application <ul><li>Addition of Bt gene from Bacillus thuringiensis, as insecticide. </li></ul><ul><li>First whole GM food (Flavr Savr) </li></ul>05/26/09
  10. 10. GMC <ul><li>Selective breading </li></ul><ul><li>slow </li></ul><ul><li>imprecise </li></ul><ul><li>modification of genes occurs naturally </li></ul><ul><li>uncontrollable crops </li></ul><ul><li>Hidden traits </li></ul><ul><li>GE </li></ul><ul><li>very fast </li></ul><ul><li>precise </li></ul><ul><li>we introduce genes into an organism </li></ul><ul><li>increased food security for growth challenges </li></ul><ul><li>complete coding genome knowledge </li></ul>05/26/09
  11. 11. GMC <ul><li>Increase yields & more efficient use of lands </li></ul><ul><li>Increase the tolerance to adverse growing conditions </li></ul><ul><li>Reduced maturation time & Longer shelf life </li></ul><ul><li>Improve the nutrient composition </li></ul><ul><li>Improved resistance to disease, pests, weeds and herbicides </li></ul><ul><li>Improve sensory attributes </li></ul><ul><li>Improve processing characteristics so as to reduce wastage and environmental bills. </li></ul><ul><li>Eliminate allergenicity </li></ul><ul><li>Phytochemicals enhanced </li></ul><ul><li>Saves money and promotes higher profits </li></ul>05/26/09
  12. 12. Effects of GMC <ul><li>Production of toxin in pollen </li></ul><ul><li>Newly formed dangerous pathogens, super weeds & super pests </li></ul><ul><li>Triggering of catastrophic ecological imbalance </li></ul><ul><li>Inevitable out-crossing of transgenics’ with natures’ </li></ul><ul><li>Unknown effect on men. </li></ul><ul><li>Domination of world food production by few companies & rich countries </li></ul><ul><li>Having enough but un fairly distributed </li></ul>05/26/09
  13. 13. Pros and Cons of labeling systems 05/26/09
  14. 14. Bibliography 05/26/09 <ul><li>The National Biotechnology Information Facility, New Mexico State University </li></ul><ul><li>The Science and Environmental Health Network, http:// </li></ul><ul><li> </li></ul><ul><li> </li></ul><ul><li>http:// </li></ul><ul><li> </li></ul><ul><li>http:// </li></ul><ul><li>Draper, D. (2002). Our Environment: A Canadian Perspective 2nd Ed . Scarborough: Thompson Canada Lmt. </li></ul><ul><li>Thank you </li></ul>