1.
A Review of the Pharmanex Biophotonic Scanner,
Supplements, the “Cady White Paper,” & Relevant
Genomic Science in New AgeLOC Products
Santa Ana, California – November 9, 2013
Louis B. Cady, MD – CEO & Founder – Cady Wellness Institute
Adjunct Professor – University of Southern Indiana
Adjunct Clinical Lecturer – Indiana University School of Medicine
Department of Psychiatry
Child, Adolescent, Adult & Forensic Psychiatry – Evansville, Indiana

2.
Background for this presentation:
• There are no “drug claims” made for any
products from NuSkin/Pharmanex
• No NuSkin/Pharmanex product– nor any
other “OTC” vitamin or supplement – is
designed to treat, prevent, mitigate, diagnose
or do anything else to or for any disease.
• Pharmanex nutritional supplements & weight
control products are designed to support
the structure and function of the human
body.
(c) 2013 Louis B. Cady, M.D. - all
rights reserved

3.
CURRENT PRACTICE OF MEDICINE:
What a patient had to say about her “specialists”:
“They just monitor my
degeneration.”

4.
Cady Wellness Institute – July 2005
The Reasons:
•
•
•
•
Conventional medical practice had failed me twice.
A lot of “psychiatric cases” WEREN’T “psychiatric.”
Nobody was integrated.
Nobody was looking at ALL of the peer-reviewed
literature.

5.
Today:
A Tale of FOUR
Guys…

6.
Learning objectives
• Importance of antioxidants (with refs)
• Origins and validity of the Biophotonic
Scanner
• The critical “three points” of the “Cady White
Paper”
• AgeLOC technology and “YGC’s” (??)
• Vitality and TR90

7.
“Streaking”, or…..
Better Buckle Up!!!
• Definition: Streaking is the act of taking off
one's clothes and running naked through a
public place. [ref:
http://en.wikipedia.org/wiki/Streaking - accessed 09
02 2013]
• “In 1973, what the press called a ‘streaking
epidemic’ hit Stephen F. Austin State University in
Nacogdoches, TX, with streakers being seen in
residence halls, at football games and at various
other on-campus locations and events, including
Spring graduation.”
(Harvard-Yale game, 2006)

8.
www.slideshare.net/lcadymd

9.
Here it is again… :)
www.slideshare.net/lcadymd

10.
Evansville Courier & Press: May 29,
2006

11.
As of 11/8/2013

12.
As of 11/8/2013

13.
As of 11/8/2013

14.
Could it happen to you?

15.
What causes oxidative stress?
• Environmental Toxins
–
–
–
–
Heavy metals – including Mercury (fillings!)
Pesticides/herbicides
Preservatives
PCB’s, Dioxins, Phthalates
• Toxins produced in the body
– Yeast and bacteria
– Products of cellular metabolism
• Emotional Stress
– Anxiety/Tension
– Fear
– Anger

16.
Oxidative stress in the BRAIN – why
you should “know your numbers”

17.
Why is the brain so
susceptible to oxidative stress?
• Burns glucose – generates free radicals.
• Uses 20% of total oxygen and energy consumed
by the body.
• ½ of this is for electrochemical nerve
transmission.
• Contains IRON
• Contains essential fatty acids, which can be
OXIDIZED (“rancid fat”)
• Limited supply of own antioxidants
• Limited ability to regenerate/repair damage

18.
•
•
•
•
What are some consequences of
oxidative stress?
Behavioral deterioration
Impaired cognitive function
Decreased memory
Psychiatric symptoms
• Increased inflammation
• Depressed immune system
• DNA damage
• cancer

19.
Antioxidant assessment
Reference:
www.cadywellness.com/cadyw

20.
Center for Biomedical Optics – Dixon
Laser Institute, University of Utah
• Werner Gellermann, Ph.D. – Research
Professor
• Colleague – Igor Ermakov, Ph.D., et al.
• Technologies – “Novel Raman Spectroscopy
and Raman Imaging.”
• Funding:
– National Institutes of Health
– State of Utah
– Spectrotek, LLC

21.
“A method and apparatus are provided
for the determination of levels of
carotenoids and similar chemical
compounds in biological tissue such
as living skin.”

22.
Dissolved carotenoids in stratum corneum
27 days

23.
Scientific Validation: Peer-reviewed by the
medical & research communities
• Society of Investigative Dermatology, May 25, 2000.
– Non-Invasive Raman Spectroscopic
Detection of Carotenoids in Human Skin.
• Arch Biochem Biophys. 2010 Dec 1;504(1):40-9. Epub
2010 Aug 1.
– Validation model for Raman based skin
carotenoid detection
• J Eur Acad Dermatol Ven. 2011 Aug;25(8):945-9.
– Skin carotenoid levels in adult patients with
psoriasis.

24.
“RSS spectroscopy is a reliable
method for non-invasive
measurement of B-carotene
levels in skin, and may be used
as an important biomarker of
antioxidant status in nutritional
and health studies in humans.”
Ramirez-Velez, et al. Endocrinol Nutr. 2012 April

25.
“But is the scanner really valid and
dependable?”

26.
• “The totality of the evidence supports the use of skin carotenoid
status as an objective biomarker of fruits/vegetable intake…”
• “..skin carotenoids may effectively serve as an integrated
biomarker of health…”
• “Thus, this biomarker holds promise as both a health
biomarker and an objective indicator of fruits & vegetables
intake….”

27.
Scamo S et al. Br J Nutr. 2013
Sep 14;110(5):911-7.
• Good agreement between single and multiple measures (95% CI)
• + association with intake of total carotenoids
• “A single RRS measure was reasonably accurate at classifying
usual skin carotenoid state.”

28.
So where do I find this
“white paper”?
• www.google.com
• www.cadywellness.com/
cadywhitepaper2008.pdf

29.
Relationships of carotenoids to other more
conventionally measured antioxidants (p 6 -7)
• Svilaas et al (2004):
– 2, 670 adults studied with serum carotenoids
measured and correlated with vitamin
consumption
– serum carotenoids were stronger predictors of
other antioxidants than were alpha, beta, delta,
and gamma tocopherols, as well as
glutathione.*
* Svilaas A, et al. Intakes of antioxidants in coffee, wine, and
vegetables are correlated with plasma carotenoids in humans. J
Nutr. 2004 Mar;134(3):562-7.

30.
Convenience of carotenoids as antioxidant
measurement (pp7-11)
• Hata, et al* (2000) – correlated skin
carotenoids (Raman) with carotenoid
levels from skin from abdominoplasty patients
(HPLC).
• “Our technique can be used…for
assessing antioxidant status and the
risk for diseases related to oxidative
stress.” [Palm of skin = highest carotenoid location]
*Hata et al. Non-invasive Raman spectroscopic detection of carotenoids in human
skin. J Invest Dermatol. 2000 Sep; 115(3):441-8.

31.
The Svilaas/Hata Syllogism:
• Hata (2000) – Raman measurements of
carotenoid == HPLC skin measurements
• Svilaas (2004) – skin carotenoids = better
predictor of antioxidant status than mixed
tocopherols and glutathione
• THEREFORE [Cady]: Raman spectroscopy
is a better measure of antioxidant status
than mixed tocopherols and glutathione.

32.
BTW – what does that “SCS” actually
mean in REAL LAB MEASUREMENTS?
• Y = 12703 * X + 5891.7
– Where Y is the SCS, and X is the carotenoid
concentration in MICROgrams/ml, viz:
•
•
•
•
•
25,000 = 12703X + 5891.7
25,000 – 5891.7 = 12703 * X
19,108.3 = 12703 * X
19,108.3 /12703 = X
X = 1.50 MICROgrams /ml
• Rule of thumb: 1,000 on SCS = .06 ug
carotenoid/ml blood serum
• (e.g., SCS of 40,000 = 2.4 ug carotenoid/ml)
Source: NuSkin/Pharmanex

33.
“You don’t need vitamins: (a) you can get’em in
your food; (b)they just make expensive urine.”
“I don’t believe in antioxidants.” – from an MD!!

34.
“Inadequate intakes may result in chronic
metabolic disruption, including mitochondrial
decay.”
“[They] cause DNA damage, such as chromosome
breaks, in cultured human cells or in vivo.”
“triage theory

35.
16,103 people – 1994 – 1996.
ZERO got 100% of all required vitamins and
nutrients
- Highest – B12 =- 82.8% got enough
- 66.8% - had adequate folate
- Lowest – Zinc – only 26.6% got enough.

36.
Seismic shift in the
medical literature - 2002
“Pending strong evidence …from randomized trials, it
appears prudent for all adults to take vitamin
supplements.” Fletcher & Fairfield, JAMA 2002

37.
What’s New since the “cady
report” (2006 & 2008)?

38.
Angela Mastaloudis Ph.D.
Senior Scientist, Global Research &
Development, Pharmanex

39.
“RS accurately measures total carotenoids in human skin
with less intra-individual variability than measurement of
serum carotenoids by HPLC analysis. RS technology
is a valid and reliable noninvasive method
to rapidly assess carotenoid nutritional
status in humans.” (J Am. Coll Nutr 2009)

40.
Epidemiology of Vascular Aging (EVA)

41.
Epidemiology of Vascular Aging (EVA)
• Study population:
– N=1,389; age range {59-71 yoa}
– 9 year study
• Relative risks:
– all cause mortality at 2.94X in men
in lowest quintile (95% CI, P=0.03)
– cancer 1.72X in men (95% CI, P=0.01
• “Total plasma carotenoids levels
were independently associated
with mortality risk in men.”

42.
Vitamin/mineral supplementation & cancer,
cardiovascular, and all-cause mortality
(EPIC-Heidelberg) Li K, Kaaks R., et al. Eur J Nutri July 2011
• Purpose: evaluate vitamin/mineral
supplementation with CA, CV dz, and mortality
•
Methods:
– 23,943 healthy participants, followed 11 years
– Baseline and “new-use” supplementation noted
•
Results: 513 CA deaths, 264 CV deaths
– “No MVI supplement had any significant effect”
• “sick user” phenomenon discussed.
• Baseline users of antioxidant
supplements had significantly
reduced risk of cancer [HR 0.52] and
all cause mortality [0.58]

43.
Antioxidants and brain tumors?
• “free radicals are another etiological factor
of brain tumor and are removed by cellular
antioxidants in the human body.”
• Inverse correlation between:
– antioxidant levels and oxidative DNA damage
– Grades of malignancy
• Decrease in antioxidants are associated
with severity of malignancy

44.
Conclusions
• “It is concluded that
administration of antioxidants
could reduce the incidence of
brain tumors and probably other
types of cancer.”
• - Shewelta SA , Sheikh, BY Curr Drug Metab. 2011
Jul;12(6):587-93. (Dept.of Biosciences & Technology,
Alexandria University, Egypt.)

45.
So why do you need a
SYSTEM in your vitamin
supplement/mineral
complex?? And what’s a
‘nano’?

46.
A SYSTEMS approach to nutritional
optimization
3
ega- s
Om cid
tty a
fa
nts
trie
ns
mi
onu
vita
hyt
P
nts
s
a
ral
xid
ine
ntio
m
lth) A
hea
one
(b

47.
What does “Nano” mean?
• Cylcodextrin ring – benign, digestable
• Feeds the gut microflora

48.
How do nano-nutrients work?
Fat-soluble nutrients like
Nano-encapsulated nutrients do
carotenoids and coenzyme Q10 not clump; they DISPERSE.
CLUMP in watery milieu.

49.
The Nanotechnology Difference
pmol/mg protein
5-fold increase in absorption!
Adapted from Craft et al, FASEB Journal. 2005; 19(4): Abstract #281.6, A449.
γ-cyclodextrin
complex

50.
The train has left the station…

51.
Can you relax with an
81,000 scan score??

52.
Total organic fruits/vegetables intake, 9
– 12 servings per day. No supplements.

53.
74,000

54.
what about other
things besides carotenoids
and “Antioxidants”??
Or… why can’t you just “juice” your way
to complete and optimal health???

55.
Essential Fatty Acids – relevant for:
“Psych”
“Medical”
•
•
•
•
•
•
• Seizures
• Rheumatoid arthritis
(& anything with
inflammation)
• Coronary heart
disease (or health
maintenance)
Depression
Bipolar depression
Schizophrenia
Autism
AD(H)D
Learning disabilities

56.
Do YOU Need Fish Oil?
5X reduction

57.
Symptoms of B12 Deficiency
Mental
•
•
•
•
•
•
•
•
•
Irritability
Apathy
Personality changes
Depression
Memory loss
Dementia
Hallucinations
Violent behavior
Anxiety
Physical
• Diminished sense
of touch and pain
• Clumsiness
• Weakness
• Pernicious anemia
• Chronic fatigue
• Tremors
• GI problems

58.
LifePak Nano - % RDA in 2 packets
• B12
1,000% (as 60 MICROgrams
cyanocobalamin)
• B6
500%
• Folic acid/folate - 150%
• Magnesium
76% (as 300 Mg amino acid chelate)
• Zinc
100% (15 mg as zinc chelate)
• Selenium
200% (L-selenomethionine & NaSeO3)
• Iodine
76%
• Chromium
166%
• Vitamin C
834%
• Vitamin A
300% (83% as Beta carotene and plant
extracts)

59.
Why there is an epidemic of B12
deficiency??
• Gastric intrinsic factor produced by parietal
cells of stomach;. H1 blockers shut them down
• Can’t absorb B-12 without it.
• Complex goes to last portion of small intestine,
where complex is absorbed and B12 liberated.

60.
Your brain and B12
• If you are in the bottom 1/3rd for B-12
levels….
• SIX TIMES increase risk of
brain volume loss (Odds ratio =
6.17, 95% CI)
– Vogiatzoglou A et al. Vitamin B12 status and
rate of brain volume loss in community-dwellers.
Neurology. 2008 Sep 9;71(11):826-32.
– (107 volunteers aged 61-87 years of age)

61.
BRAIN SHRINKAGE…
CONCLUSIONS: “The
accelerated rate of brain
atrophy in elderly with MCI can
be slowed by treatment with
homocysteine-lowering Bvitamins.”
Smith AD, Smith SM, de Jager CA, Whitbread P, et al. (2010) Homocysteine-Lowering by B Vitamins Slows the
Rate of Accelerated Brain Atrophy in Mild Cognitive Impairment: A Randomized Controlled Trial. PLoS ONE
5(9): e12244. doi:10.1371/journal.pone.0012244
http://www.plosone.org/article/info:doi/10.1371/journal.pone.0012244

62.
LifePak Nano - % RDA in 2 packets
• B12
1,000% (as 60 MICROgrams
cyanocobalamin)
HOMOCYSTEINE
• B6
500%
lowering
• Folic acid/folate - 150%
• Magnesium
76% (as 300 Mg amino acid chelate)
• Zinc
100% (15 mg as zinc chelate)
• Selenium
200% (L-selenomethionine & NaSeO3)
• Iodine
76%
• Chromium
166%
• Vitamin C
834%
• Vitamin A
300% (83% as Beta carotene and plant
extracts)

63.
OK. It slows shrinkage. But are they sharper?
[deJager CA et al. Int J Geriatr Psychiatry. 2012 June;27(6):592-600]
“B-vitamins appear to slow cognitive and clinical
decline in people with MCI.”

64.
The Effects of high dose B vitamins
on stress at work
• 3 month, double blind, placebo control, randomized study
– Measured: Personality, work demands, mood, anxiety, and strain
• The vitamin B complex treated
group reported significantly lower
personal strain and a reduction in
confusion and depressed/dejected
mood after 12 weeks.
Strough C et al. The effect of 90 day administration of a high dose vitamin Bcomplex on work stress. Hum Psychopharmacol. 2011 Sept 8. doi
10.1002/hup.1229 (Swinburne University of Technology – Hawthorn, Victoria,
Australia.)

65.
“Micronutrient supplementation has a beneficial
effect on perceived stress, mild psychiatric
symptoms, and aspects of mood in apparently
healthy individuals. Supplements containing high
doses of B vitamins may be more effective in
improving mood states.”

66.
What happens with biotin
deficiency?
Two LifePak Nano packets have
100% of recommended daily
requirements of Biotin.
“Our results suggest that biotin deficiency
promotes hyperglycemic mechanisms…and
decreased insulin secretion and sensitivity to
compensate for reduced blood glucose
concentrations.”

67.
One more time… no supplement, under the DSHEA act, may
be represented to treat, cure, mitigate or prevent any disease.
No such “health claims” are being advanced for any
Pharmanex/NuSkin supplement or product. The following
slides represents a LITERATURE REVIEW It correlates
antioxidants, carotenoids and various conditions described in
the peer reviewed medical literature.
- Louis B. Cady, MD
On November 8th, 2013
More data: a mad dash through
the literature

68.
“Chile has an increased cancer prevalence which is associated
with poor quality diets, rising mean body mass index and a
sedentary behavior.”

69.
100 more citations in the last year!

70.
OK.
So I’ll just drink
carrot juice and
pop some high
potency B’s. What
me worry?

71.
20 years ago - a hint of bad things to come…
• North Americans, unfortunately,
obtain about one-third of their total
calories from nutrient-poor junk food.
Even well-educated… adults…. fall
short in their consumption
of important minerals, like
magnesium, iron, zinc, and
calcium.
Hallfrishch, J., Muller, D. 1993. Experimental
Gerontology, v28 : 473-83.

72.
Magnesium mementos
• One of the first minerals to disappear with:
– Processed food
– Stress
• Decreased by EtOH, caffeine, sodas,
meds
• Considered “anti-stress” mineral
– Decreases cortisol (rel to sleep disruption)
– Relaxes muscles, prevents cramps
– Decreases anxiety; improves sleep

73.
Symptoms of Magnesium Deficiency
PSYCHIATRIC ISSUES:
•Difficulty with memory and
concentration
•Depression, apathy
•Emotional lability
•Irritability, nervousness,
anxiety
•Insomnia
Autism
ADHD
Health Conditions Associated
with Magnesium Deficiency

Migraine headaches
 PMS
 Dysmenorrhea
 Fibromyalgia
 Fatigue
 CONSTIPATION

74.
SELENIUM DEFICIENCY in FASEB:
• “Adaptive dysfunction of
selenoproteins from the
perspective of the ‘triage’
theory: why
modest
selenium
deficiency may
increase risk of
diseases of aging.”
Foundation of American
Societies for Experimental
Biology
McCann, J, Ames BM. FASEB J.
2011 Jun;25(6):1793-814.

75.
I+
Se+

“the foot soldier”
LifePak Nano has 200% of RDA
of selenium.
And 76%of RDA of iodine.

76.
 “Recent
years have seen a
SUBSTANTIAL, and as yet
UNRECOGNIZED ageindependent population-level
decrease in T in American
men.” November 2009
Travison, Araujo, et al. Jrnl of Clin. Endocrinol & Metabol 92:1; 196-202.
3 cohorts of men: 1987-1989; 1995-1997; 2002 -2004.
“Alpha Male” issue

77.
Fast food (low
Zinc) is bad for you.
• Fast food = high energy density = low essential
micronutrient density, ESPECIALLY ZINC
• Antioxidant processes are dependent on Zinc
• Fast food = severe decrease in antioxidant
vitamins and zinc, correlating with
inflammation in testicular tissue – with
underdevelopment of testicular tissue and
decreased testosterone levels

78.
Low Zinc associated with low
testosterone
• Per USDA, 60% of US men between 20
– 49 years of age do not get enough.
– [N.B.: Do not supplement with > 50 mg daily
(can interfere with Cu+ metabolism); rationale
for SYSTEMS approach]
• Tsai, E.C., Boyko, E.J., Leonetti, D.L., & Fujimoto,
W.Y. (2000). Low serum testosterone level as a
predictor of increased visceral fat in JapaneseAmerican men. International
Journal of Obesity and Related Metabolic Disorders,
24, 485-491
LifePak Nano has 100%
of daily RDA for Zinc.

79.
Erectile dysfunction & antioxidants
with MVI’s??

80.
Ways to improve erectile quality
(LP Nano concentrations in parentheses)
• Holistic: physical exercise, improved nutrition,
weight control (R90 & TR90)
• Supplements: adequate intake
– Omega 3 fatty acids ( ~4 grams/ day in LP Nano)
– Antioxidants {+++}
– Calcium (600 mg = 60%)
– Folic acid (folate – 600 ug = 150%)
• RX: Replacement of any T deficiency
Meldrum DR et al. The link between erectile and cardiovascular health: the
canary in the coal mine. Am J Cardiol. 2011 Aug 15;108(4):599-606

81.
New Partners, New Products

82.
LifeGen acquisition: Why?
• Owns all current and future genetic data
on aging
– monopolizes the genetic knowledge on aging
• Applies LifeGen science to all products
– Unrestricted access
• Builds barrier to entry
• Enhances Nu Skin product innovation in
antiaging (“Apple” of the industry)
99

83.
Is it safe to Influence Genes?
ageLOC is NOT:
gene therapy
genetic engineering
gene splicing
ageLOC is:
• NuSkin/Pharmanex - novel approach to
developing products designed to support
youthful gene expression.

84.
An Entirely New Class of Anti-aging Products

85.
BEFORE
AFTER 6 MONTHS
*Twice daily usage of ageLOC
Transformation, and twice weekly
usage of ageLOC Galvanic Spa and
Facial Gels.

86.
Mitochondrial Damage:
Loss of Energy
Mitochondria
Free radical
damage alters
their energy
production
capability. Plus,
they “breathe
their own
exhaust.”

87.
MITOCHONDRIAL ACTIVITY DECLINES WITH AGE
8
6
- 40%
4
2
18~39
61~84
”Mitochondrial Dysfunction in the Elderly: Possible Role in Insulin
Resistance” Science 16 May 2003:Vol. 300. no. 5622, pp. 1140 – 1142

88.
“Transcriptional Biomarkers of Mitochondrial Aging and
Modulation by Cordyceps sinensis CS-4.”
Muscle
Brain
R Weindruch, A Mastaloudis, SB Ferguson et al. Presented at Biology of Aging,
Determinants of Health-Span. Switzerland August 22-27,2010

89.
TARGET & RESET YOUTH GENE
CLUSTERS
Young Gene
Expression
TARGET
Old Gene
Expression
Old Gene
Expression
RESET
Transcriptional Biomarkers of Mitochondrial Aging and Modulation by Cordyceps Sinensis Cs-4. Gordon Research
Conference, Biology of Aging, Determinants of Health-Span: From Cells to Humans, August 22-27, 2010. Les Diablerets
Conference Center, Les Diablerets, Switzerland.
1

90.
“Youth Gene Clusters” – bogus
or bonanza?
Yeast species
SPECIFIC genes
Comparative genomics of fungal allergens
and epitopes show widespread distribution of
closely related allergen and epitope
orthologues. Bower P, et al, BMC
Genomics. 2006; 7:251.

91.
Prostate Cancer – more
relevant?
Pre-invention gene
expression
Post-intervention
gene expression
Changes in prostate gene expression in
men undergoing an intensive nutrition
and lifestyle intervention. Ornish, Dean
Proc Natl Acad Sci USA
2008 June 17; 105(24):8369-8374
et al.

92.
Active mitochondrial research on
energy

93.
http://www.nature.com/scitable/topicpage/mtdna-andmitochondrial-diseases-903

94.
Three active botanicals:
•Cordyceps
•Pomegranate extract
•Panax ginseng
Promotes Three Dimensions
of Vitality:*
•Physical Vigor
•Mental Acuity
•Sexual Health
* These statements have not been evaluated by the Food and Drug Administration. This product
is not intended to diagnose, treat, cure, or prevent any disease.

95.
Week 12 = +20%
P=0.021
Vitality vs. Placebo
“Targeting Age-related gene expression improves
mental and physical vitality” Ferguson SB SM et al.
Paper presented at 1st World Congress on Targeting
Mitochondria, Strategies, Innovations & Clinical
Applications. Berlin, German. November 18-19, 2010.

96.
“Excess body weight is correlated with excess morbidity
and mortality. In particular, Grade 2 or higher obesity
(BMI>35) significantly increases the risk of death.”
(c) 2013 Louis B. Cady, M.D. - all
(www.cdc.gov/nchs/data/hus/hus11_InBrief.pdf)
rights reserved

97.
Obesity – a national crisis
A $586 Billion Market in
2014
Fortune Magazine Feb 3, 2003

98.
How do you lose it?

99.
• TBL: It’s the
INSULIN
RESPONSE
• RX: EAT LESS
CARBS AND
SUGAR!!
NEW APPROACH:
-Turn on your genes in your fat cells to burn more
fat and store less fat!!
- Change hunger signaling to brain by changing
gene expression

100.
ageLOC TR90 Summary
• Healthy weight management and Body
Shaping program.
• Based on gene expression science.
– Promotes Lean Muscle Maintenance
- Appetite Control
• Studies on TR90 weight management
program resulted in significant weight loss,
fat loss and changes in gene expression.

101.
Comparison of Genetic Profiles of Adipose Tissue
in Identical Twins
Pietiläinen et al. PLoS Med. 2008 Mar 11;5(3):e51.
Overweight Twin
Normal Weight Twin
Same DNA
Different Expression of Genes
Compared to their normal weight twin, overweight twins exhibited down
regulation of gene expression pathways related to:
•
•
Fat Regulation (storage versus utilization)
Muscle Protein Synthesis

102.
Identified Gene Expression Pathways Linked to
Fat Storage and Fat Metabolism
LifeGen pre-clinical study of Adipose,
Gastrocnemius muscle, Hypothalamus

103.
Plants altering gene expression
Ingredient
Effect
From PubMed
Prickly pear fruit
“body silhouette”
Potent antioxidant
Green tea catechins
Thermogenesis
(Established)
pomegranate
Fat reduction
related to the presence of
anthocyanins, tannins, and
very high levels of
antioxidants, including
polyphenols and flavonoids.
Tart cherry
Prevents muscle
damage
increasing total antioxidative
capacity, reducing
inflammation, lipid
peroxidation

104.
Green tea, black tea, epigallocatechin:
•Modifies body composition
•Improves glucose tolerance
•DIFFERENTIALLY ALTERS GENE
EXPRESSION

105.
Gene Expression relates to the Appetite Control
Center of the Brain
•
•
•
Appetite Control
Eating Behavior
Regulation of energy intake
Ghrelin
Leptin
Insulin
References:
1.
2.
3.
4.
5.
6.
7.
8.
9.
Smeets et al. Proceeding of the Nutrition Society. 2012;71:511-520.
Guthoff M, et al. J Clin Endocrinol Metab. 2010;95:748-755.
De Silva, et al. Cell Metab. 2011;14:700-706.
Malik S, et al. Cell Metab. 2008;7:400-409.
Farooqi IS, et al. Science. 2007;317:1355
Suzuki K, et al. Experimental Diabetes Research. 2012:1-19.
Viquerie N, et al. Diabetologia. 2005;48(1):123-131.
Mager et al. European Journal of Endocrinology. 2008;158:
Mutch DM, et al. Am J Clin Nutr. 2011;94(6):1399-1409.

106.
Eating Plan Based on:
Protein Science & Nutrition Science
• “3-2-1 eating” plan designed to
complement the supplements.
• Supports metabolically-active
lean muscle and inhibits fat
storage.
Reference: Wycherley TP, et al. AJCN. 2012;96(6):1281-98.

107.
Eating Most of Your Protein with Only One Meal of the
Day Limits Duration of Metabolic Burn
FAT STORING
ZONES
FAT BURNING
Time in Metabolic Burn Zone = Only 3 hours/day
Breakfast
Lunch
Adapted from:
1.Paddon-Jones and Rassmussen. Curr Opin Clin Nutr Metab Care, 2009;12:86.
2.Layman DL. Nutrition and Metabolism, 2009; 6:12.
Dinner

108.
Eating Protein with Each Meal
Accelerates Metabolic Burn
FAT STORING
ZONES
FAT BURNING
Time in Metabolic Burn Zone = 9 hours/day
Breakfast
Lunch
Adapted from:
1.Paddon-Jones and Rassmussen. Curr Opin Clin Nutr Metab Care, 2009;12:86.
2.Layman DL. Nutrition and Metabolism, 2009; 6:12.
Dinner

109.
So what the
heck am I
supposed to
do with this
stuff?

110.
A personal close:
How do YOU want to age?
Me – Jan 2013

111.
The “First Guy” on TR90
• Measurements from 10/25/13 (start date) –
11/6/13
• Lost:
– ¾” on waist (one belt loop)
– 1.25” total
– 4.4 lbs
• In 10 days

112.
Wrapping Up: The “Survival Curve” –
Where Are You?

113.
“For me, the practice of medicine has
opened the door to the greatest adventure in
life. Medicine is like a hallway lined with
doors, each door opening into a different
room, and each room opening
into another hallway,
again lined with doors.
Medicine is always
wonderful and never will
be finished.”
- Charles H. Mayo, M.D.