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Natural transformation


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Extra Credit project for Natural Transformation in bacteria

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Natural transformation

  1. 1. Principle Features of Transformation Lauren Welker Biology 302 Dr. Hope Johnson
  2. 2. 1. DNA in the cell’s environment binds to the cell surface at several sites.
  3. 3. 2. One strand enters the cell, the other isdestroyed by a transformation specificnuclease.Bound DNA is nicked and cut.
  4. 4. 3. Single Stranded DNA is coatedwith DNA binding proteins toprotect it from nucleases.
  5. 5. 3. The entering DNA displaces anexisting strand forming aheteroduplex ** side notes**- If single stranded DNAis homologous to thecell’s DNA, homologousrecombination can occur.- Only transforming DNAthat is closely related tothe recipient’s DNA iscapable of transformingbecause it must be ableto hybridize with DNA ofrecipient’s genome.
  6. 6. THE END.