INFERTILITY OVERVIEWInfertility means difficulty in conceiving (becoming pregnant) despite havingregular sex without using contraception. There is no definite cut off point to saywhen a couple is “infertile’ many couples take several months to conceive. About17 in 20 couples conceive within a year of trying. About 19 in 20 couples conceivewithin two years.Looking at this another way, about 1 in 7 couples do not conceive within a year oftrying. However, more than half of these couples will conceive over the next yearwithout any treatment.
IUI (INTRA UTERINE INSEMINATION)• An intrauterine insemination (IUI) is a procedure in which the sperm is• “washed” and prepared then deposited into the upper portion of the uterus tohelp achieve a pregnancy. It is often in excellent option for couples prior toconsidering more complicated and very expensive procedures, such as in vitrofertilization(IVF).• The wash and IUI can be very helpful to couples seeking to have a child when theman has low sperm counts, low motility, hyper viscosity (very thick semen), motilesperm that are clumped together (antibodies), sperm that “ backs up” into thebladder (retrograde ejaculation) or other abnormal sperm test such a poor spermshape (morphology). Many couples use IUI to accelerate the chances of pregnancy,such as after vasectomy reversal or if the woman is older.• It is also useful when the woman’s mucus is hostile or compatible with man’ssperm, or if she has problems with her cervical positioning or opening, as well asunexplained causes of infertility.
IUI (INTRA UTERINE INSEMINATION) Methods of Sperm Preparation.1. The fundamental objective of the various preparation procedure employed isto separate the spermatozoa from the seminal plasma as thoroughly aspossible with minimum mechanical force. These are adequate for normalsemen sample. However, as the quality of reducing the level of physicaldamage inflicted upon the spermatozoa and at the separating thecontaminating material. The modifications fall into the following basiccategories. The basic aim of semen washing / processing is as follows:1. Removal of seminal plasma.2. Removal of non motile sperms, morphologically defective and immaturesperms.3. Removal of debris and pus cells.4. Concentration of motile sperms.5. Capacitating of motile sperms.
EQUIPMENTS REQUIRED FOR AN ANDROLOGY LAB 1. LAMINAR AIR FLOWLAVA ANDROLOGY WORKSTATION LAVA workstation offers possibility to controltemperature & cleanliness. Fully made of MS with powder coat finish Pre-filter, Minipleat(separator less) HEPA filters Imported blowers with noise level ofless than 55dBA. Inbuilt Incubator for semen storage Image processing system along with softwarefor IUIIUI (INTRA UTERINE INSEMINATION)
EQUIPMENTS REQUIRED FOR AN ANDROLOGY LAB. 2. TRINOCULAR MICROSCOPELABOMED LX 300 Adapted for all types of lab environments, the new Lx 300 reflects afresh design and delivers a rugged exterior with quality optical andmechanical ingredients for a lifetime of carefree use. This nextgeneration microscope asserts its sophistication not only by design,but also through flawless functionality. In light of the latest inmanufacturing technology, the highly modular Lx 300 can offer you ahigh degree of performance for various applications. Our LP series objectives enable you to view an assortment ofspecimens with the highest degree of clarity. Lx 300’s Plan Achromaticoptical system has been With a compact design and robust mechanical stage with fluid X/Yoperation, maintenance free is a standard. Lx 300 offers you flexibility in illumination. You can stay withthe standard 20W high efficiency halogen bulb, or you can opt for aBright LED, providing illumination in a near daylight spectrum, with Battery Back.IUI (INTRA UTERINE INSEMINATION)
3. SPERM COUNTING CHAMBER The Makler Counting Chamber is a simple to usedevice for Sperm Count and motility evaluationfrom undiluted semen. Depth of only 10 microns.Constructed with two pieces of optically flat glass,cover glass upper, 2nd layer with a 1sq.mm finde gridin the centre subdivided into 100 squares of0.1x0.1 mm each. Percentage and quality of motilityand sperm counts are easily obtained.IUI (INTRA UTERINE INSEMINATION)
4. CENTRIFUGE R-4C Compact model is designed for routine work inmedical and industrial laboratories. It has digital speedindicator and 0-60 minute digital countdown timer. R-4C DX has additional features of dynamic brake forquick deceleration & imbalance detector with cutoffin case of uneven loads.Salient Features: Stepless speed regulator Safety Lid interlock to prevent cover opening duringcentrifugation Dynamic brake for quick deceleration (R-4C DX) Imbalance detector with cutoff (R-4C DX)IUI (INTRA UTERINE INSEMINATION)
5. HEATING BLOCK Fully made out of SS 304 – dully polished. OMRON German controller for better accuracy. 3 no’s of anodised aluminium blocksinterchangeable with workstation blocks. Each block can hold 6 no’s of 14ml falcon tubes.IUI (INTRA UTERINE INSEMINATION)
6. IUI MEDIAS – GYNAMED1. GM 501 SPERM ACTIVE – SWIM UP2. GM 501 GRADIENT - DOUBLE DENSITY GRADIENT1. SPERM PROCESSING MEDIA GM501 SPERM ACTIVEGM501 Sperm Active is a ready-to-use medium designed for allsperm-preparation, sperm-washing and swim-up techniques likedensity-gradient centrifugation as well as for testicular tissue.2. SPERM PROCESSING MEDIA GM501 GRADIENTGM501 Gradient is a saline-silica based density gradient medium usedin ART for separation and purification of highly motile humanSpermatozoa. A dual density Gradient system (45% and 90%) startingfrom GM501 Gradient 100% If preferred, a multi-layer may be usedas well (e.g. 45%-70 %-90 %).IUI (INTRA UTERINE INSEMINATION)
7. SAFE 3.1 - Software Assisted Fertility carE 3.1 Andrology software for Semen Analysis, Processing (pre wash / post wash) & report.IUI (INTRA UTERINE INSEMINATION)
IVF (IN VITRO FERTILISATION) In vitro fertilisation (IVF) is a process by which an egg is fertilised by sperm outsidethe body: in vitro. IVF is a major treatment for infertility when other methods ofassisted reproductive technology have failed. The process involves monitoring awomans ovulatory process, removing ovum or ova (egg or eggs) from thewomans ovaries and letting sperm fertilise them in a fluid medium in a laboratory.When a womans natural cycle is monitored to collect a naturally selected ovum(egg) for fertilisation, it is known as natural cycle IVF. The fertilised egg (zygote) isthen transferred to the patients uterus with the intention of establishing asuccessful pregnancy. The first successful birth of a "test tube baby", Louise Brown,occurred in 1978. Louise Brown was born as a result of natural cycle IVF. Robert G.Edwards, the physiologist who developed the treatment, was awarded the NobelPrize in Physiology or Medicine in 2010.
ICSI (INTRA CYTOPLASMIC SPERM INJECTION)• ICSI is generally performed in addition an in vitro fertilization procedure to extractoften several oocytes from a woman.• The procedure is done under a microscope using multiple micromanipulationdevices (micromanipulator, microinjectors and micropipettes). A holding pipettestabilizes the mature oocyte with gentle suction applied by a microinjector. Fromthe opposite side a thin, hollow glass micropipette is used to collect a singlesperm, having immobilised it by cutting its tail with the point of the micropipette.The oocyte is pierced through the oolemma and into the inner part of the oocyte(cytoplasm). The sperm is then released into the oocyte.
IVF/ICSI - PROCEDURE• CONTROLLED OVARIAN HYPERSTIMULATION FOR MULTIPLE FOLLICLEDEVELOPMENT.• MONITORING OF THE TREATMENT CYCLE.• OOCYTE RETRIVEAL.• FERTILIZATION INVITRO.• EMBRYONIC DEVELOPMENT IN VITROA. LASER ASSISTED HATCHINGB. CRYO PRESERVATION• EMBRYO TRANSFER• LUTEAL SUPPORT• FOLLOW UP
STAR IVF WORK STATION Fully made of MS with powder coat finish. Pre-filter, Minipleat (separator less) HEPA filters. Imported blowers with noise level of less than 55dBA. State-of-art Heating System for Table and Stage Heating Block Integrated Customize Heating Areas using the standard availableheat patterns. Integrated Stage Warmer with stereo zoom mountingaccording to your microscope model.IVF/ICSI - PROCEDURE
IVF/ICSI - PROCEDURE OOCYTE COLLECTION -ASPIRATION PUMP Digital display of set & actual vacuum. Digital Low volume Vacuum pump. Uniform flow pattern. Max Vacuum attained 400mm of Hg. With overflow bottling & battery backup. Wired & wireless foot pedal.
IVF/ICSI - PROCEDURE STEREO ZOOM MICROSCOPE OLYMPUS SZX 10The SZX10 is designed for routine research. It offers a highnumerical aperture (NA) of 0.2, producing a resolution of600 line pairs per millimeter. With this resolution you canwork more efficient, more precise and gain much moreinformation from your samples. The SZX10 provides a zoomratio of 10:1. With the common objective design it is a fullymodular system offering a range of options.
IVF/ICSI - PROCEDURE CO2 INCUBATOR – MEMMERT - IN 108 (BOX TYPE) Certified Medical device. Sterilization Temp.:160° C for 4 hrs. IR CO2 Sensor. No need to remove any sensors during Sterilization. 2 Pt100 sensors Class A in 4-wire-circuit,mutually monitoringand taking over the performance at the same temperaturevalue. Electro polished interior seamlessly welded by laser. Integrated humidity limit control (88-97%) with digital displayof relative humidity – resolution of display : 0.5%, settingaccuracy:1%. Adaptive, fuzzy-supported, multifunctional, digital microprocessorPID-controller. Digital display (LED) of all set parameters, such as temperature, weekdays, time, CO2, humidityand set-up values - language to be chosen in setup. Independently working, digitally adjustable electronic over temperature controller TWWprotection class 3.1. intrinsic adjustment of altitude above sea level.
IVF/ICSI - PROCEDURE TRI GAS INCUBATOR – K-SYSTEMS - G185 (BENTCH TOP) 6 separate sensors to control and regulate temperature . CO2 & O2 gas environment can be adjusted. CO2 Range : 2-10% : O2 Range : 2-20%. Grade A air quality. Circulating through UV lightchamber; HEPA/VOC filter. Fast recovery : CO2 < 2min : O2 < 4min. 10 chambers each accompanying 4 dishes. Individual gas flow for each chamber. Easy cleaning – warming blocks can be autoclaved. Minimised risk of cross contamination, 10 microenvironment chambers.
IVF/ICSI - PROCEDURE CARL ZEISS WITH S MICROMANIPULATOR Carl Zeiss Microscopy GmbH – Germany inverted microscope Axio vert.A1. 13mm focus lift with adjustable focus stop. Binocular phototube, 45°/23 (50:50) Green Filter, d=32x2mm. Neutral density filter0.06. Optical equipment phase contrast and plasDIC. Objective LD A-plan 5x/0.12 ph 1 M27,10x/0.25 ph 1 M27 & 20x/0.35 ph 1 M27. LD Condenser 0.4 for slider, for H, Ph, plasDIC,VAREL for objectives 5x – 63x 53mm workingdistance. Camera Adapter 60N-C 2/3” 0.5X. S-micromanipulator: MO-20 3 Dimensional with joy-stick (Micromanipulator) Universal Joint, X/Y/Z 20mm each, 2mm one revolution handle, joy-stick on X/Y plane 2mm/max. IA-1 Pneumatic injector. Injection holder set, 15mm.
IVF/ICSI - PROCEDURE OKO LAB – T GLASS Transparent and flat surface at controlled temperature. Built in temperature sensor with 3.2” touch screen PID controller. External temperature sensor for self calibration routine to obtainmedia temperature on petri plate. Temperature range : from room to 40°C Temperature accuracy on specimen : + 0.3°C Glass thickness : 0.5 mm System features:PLATE : #1 round heated plate with built in temperaturesensor.CONTROLLER : Multichannel temperature controller.with 3.2” touch screen and Self Calibration Routine.#1 External temperature sensor.RS232 for Data Logging (Optional)Data Logger (Optional)
SAFE - ET - Software Assisted Fertility carE 3.0Embryo Transfer software.IUI (INTRA UTERINE INSEMINATION)
IVF/ICSI - PROCEDURE VOC METER Real time monitor ambiance air quality. Semiconductor mix gases sensor with 5 year life. Gas detection : cigarette smoke, VOC’s such as formaldehyde andToluene, ethanol, ammonia, hydrogen sulfide, sulfur dioxide andOther harmful gases. Monitor temperature and relative humidity. Three-color (Green/Yellow/Red) LCD backlit indicating air qualityAt optimal/moderate/poor. Provide 1x on/off output to control a ventilator. High quality technics, elegant appearance & CE-approval.
IVF/ICSI - PROCEDURE DYLOS AIR QUALITY MONITOR – DC1100 A true Laser Particle Counter with 2 size ranges1. small (bacteria, mold, etc.) 2. large (pollen, etc.) DC1100 features technology and engineering that allowsmonitoring of indoor air quality with an LCD screen thatprovides small and large particle counts with a dynamic bargraph showing actual count reading. Multiple modes including minute, hour, day and monitor toevaluate your air quality. Store up to 30 days of air quality history for review.
IVF/ICSI - PROCEDURE DYLOS AIR QUALITY MONITOR Specially designed for the continuous monitoring of CO2 incubators. Improved accuracy on CO2 readings. Quick verification of CO2 incubator levels. Time saving with dual temperature probes option. Large data storage and user friendly software and download. Easy to read large well lit display. CO2 measuring range 0-20%. Optional : Temperature probe for incubatorOxygen moduleHumidity module
IVF/ICSI - PROCEDURE DIGITAL THERMOMETER 0.1 ° C resolution over the full range. Max/min, hold & auto power off facility. Range of -20 to + 50°C. Resolution of 0.1 °C of 0.1 °F. Thermometers never need re-calibration.
IVF/ICSI - PROCEDURE CRYO PRESERVATION UNIT Embryo cryopreservation is useful for leftoverembryos after a cycle of in vitro fertilisation, aspatients who fail to conceive may becomepregnant using such embryos without having togo through a full IVF cycle. Or, if pregnancyoccurred, they could return later for anotherpregnancy. Spare oocytes or embryos resultingfrom fertility treatments may be used for oocytedonation or embryo donation to another womanor couple, and embryos may be created, frozenand stored specifically for transfer and donation by using donor eggs and sperm.