Primer design

1,007 views

Published on

primer design ,help youshelf

Published in: Education, Technology, Business
0 Comments
2 Likes
Statistics
Notes
  • Be the first to comment

No Downloads
Views
Total views
1,007
On SlideShare
0
From Embeds
0
Number of Embeds
1
Actions
Shares
0
Downloads
77
Comments
0
Likes
2
Embeds 0
No embeds

No notes for slide

Primer design

  1. 1. 1. PCR primer design• Local software Primer premier5, Oligo 6.1• Web-based design Primer3 (Whitehead Institute/MIT Center for Genome Research)
  2. 2. Primer premier5
  3. 3. Principles for primer design• Base composition• Length• Repeated and self-complementary seq.• Cross dimer• Tm• 3’ Termini• Priming sites (From Molecular cloning 3, 8.14)

×