Electron microscope


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Electron microscope

  1. 1. Electron Microscope Presenter : Khizra Samad
  2. 2. • An electron microscope (EM) is a type of microscope that uses an electron beam to illuminate a specimen and produce a magnified image. • An electron microscope has greater resolving power than a light microscope and can reveal the structure of smaller objects because electrons have wavelengths about 100,000 times shorter than visible light photons. They can achieve better than 50 pm (1x10 raise to power -12 of a meter) resolution and magnifications of up to about 10,000,000x (10 millions)
  3. 3. Cont.. • The first electromagnetic lens was developed in 1926 by Hans Busch ,was a German physicist. He was a pioneer of electron optics and laid the theoretical basis for the electron microscope.
  4. 4. Uses • Electron microscopes are valuable tools in medical and biological fields, as well as for materials research. • scientists uses an electron microscope for their research. scientist cannot conduct their work without the use of an electron microscope at their lab. • INTERPRETATION OF EM DATA • USED FOR VIRUS DETECTION • Used to examine cells, microorganisms, metals, crystals and biopsy samples
  5. 5. How it works: • The original form of electron microscope, the transmission electron microscope (TEM) uses a high voltage electron beam to create an image. The electron beam is produced by an electron gun, commonly fitted with a tungsten filament cathode as the electron source. • The electron beam is accelerated by an anode typically at +100 k eV (40 to 400 keV) with respect to the cathode, focused by electrostatic and electromagnetic lenses, and transmitted through the specimen that is in part transparent to electrons and in part scatters them out of the beam.
  6. 6. Cont.. • When it emerges from the specimen, the electron beam carries information about the structure of the specimen that is magnified by the objective lens system of the microscope. The spatial variation in this information (the "image") may be viewed by projecting the magnified electron image onto a fluorescent viewing screen coated with a phosphor orscintillator material such as zinc sulfide. • Alternatively, the image can be photographically recorded by exposing a photographic film or plate directly to the electron beam, or a high-resolution phosphor may be coupled by means of a lens optical system or a fibre optic light-guide to the sensor of a CCD (charge-coupled device) camera. The image detected by the CCD may be displayed on a monitor or computer.
  7. 7. Reference • http://hearinghealthfoundation.org/electron_m icroscope • http://cmr.asm.org/content/22/4/552.full • http://en.wikipedia.org/wiki/Electron_microsc ope • http://www.ehow.com/list_6506948_electronmicroscope-uses.html