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Point of care testing
Dr. Kamal Modi
Definition
When a test is performed at a time
At which test results enables a decision to be made
And an action taken
That...
patient
doctor
request
phlebotomy
transport
register
prepare
analysis
Quality control
validate
report
transmit
interpret
Advantages
Reduced turn around time
Improved patient morbidity & mortality
Reduction in hospital admission
Improved intera...
Disadvantages
Increase in administrative work associated with training and
Certification of operators
Caregivers required ...
Classification of types
Single use Multiple use quantitative
Cartridge/ bench-top devices
Qualitative
cartridge/strip
Quan...
Analytical Principle
 Reflectance
 Lateral flow or flow through immunoassays
 Electrochemistry
 Electrical impedance
Reflectance
e.g.
Urine and blood
dipsticks for glucose and
various other analytes.
Electrochemistry
Strip device
Cassette and bench-top
device
Glucose strip
A. Electrode sensors
B. Separating layer
Principle of Electrochemical Glucose strip
Glucose meter
A negative voltage of –0.4 V is applied at the reference electrode.
When blood or a glucose solution is plac...
Bench-top Devise
Principle
Measurement of
PH, PCO2, PO2,
Electrolyte, Glucose,
Lactate.
Cassette device
Lateral flow immunoassays
It is a biological sensors in
which the recognition
agent is an antibody that
binds to the analy...
Electric Impedance
• Based on Coulter principle
• As fluid containing particles
or cells is drawn through
each micro-chann...
Unresolved Queries ???

Use of following principle in
POCT

Light scattering / optical motion

Immunoturbidometry

Flu...
Point of care testing
Point of care testing
Point of care testing
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Point of care testing

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for just understanding various POCT devices and their basic principles.

Published in: Health & Medicine

Point of care testing

  1. 1. Point of care testing Dr. Kamal Modi
  2. 2. Definition When a test is performed at a time At which test results enables a decision to be made And an action taken That leads to an improved health outcome.
  3. 3. patient doctor request phlebotomy transport register prepare analysis Quality control validate report transmit interpret
  4. 4. Advantages Reduced turn around time Improved patient morbidity & mortality Reduction in hospital admission Improved interaction between patient and carer Reduction in clinical visits Improved cost of care Reduction in administrative work associated With test requesting & reporting
  5. 5. Disadvantages Increase in administrative work associated with training and Certification of operators Caregivers required to perform test Increased risk of errors
  6. 6. Classification of types Single use Multiple use quantitative Cartridge/ bench-top devices Qualitative cartridge/strip Quantitative cartridge/strip With reader device
  7. 7. Analytical Principle  Reflectance  Lateral flow or flow through immunoassays  Electrochemistry  Electrical impedance
  8. 8. Reflectance e.g. Urine and blood dipsticks for glucose and various other analytes.
  9. 9. Electrochemistry Strip device Cassette and bench-top device
  10. 10. Glucose strip A. Electrode sensors B. Separating layer
  11. 11. Principle of Electrochemical Glucose strip
  12. 12. Glucose meter A negative voltage of –0.4 V is applied at the reference electrode. When blood or a glucose solution is placed in the strip, a chemical reaction occurs inside it, generating a small electrical current proportional to the glucose concentration
  13. 13. Bench-top Devise
  14. 14. Principle Measurement of PH, PCO2, PO2, Electrolyte, Glucose, Lactate.
  15. 15. Cassette device
  16. 16. Lateral flow immunoassays It is a biological sensors in which the recognition agent is an antibody that binds to the analyte. e.g. measurement of troponin T , myoglobin, and D-dimer.
  17. 17. Electric Impedance • Based on Coulter principle • As fluid containing particles or cells is drawn through each micro-channel, each particle causes a brief change to the electrical resistance of the liquid. • The counter detects these changes in electrical resistance. • E.g. complete blood count
  18. 18. Unresolved Queries ???  Use of following principle in POCT  Light scattering / optical motion  Immunoturbidometry  Fluorescence  Multiwavelength spectrophotometry

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