Juan Carlos Torres Sánchez #804-11-9230 BIOL 3095 Seminar 2 on Proteomic BiomarkersOn September 16 and 17, we had a seminar with Ms. Lisandra Santiago. Theconference exposed me tonew concepts that I didn’t have knowledge on, such as massspectrometry, proteomics, and biomarkers. Her approach on the topic was very good,because it intrigued me to find more information about proteomic biomarkers.,Biomarkers help usfind sets of proteins encoded by a genome that will help tracecancers.In this paper, we can see how biomarkers are so important in our life, andalsothe importance ofusingmass spectrometry for the discovery of protein biomarkers. Tofind potential biomarkers they used a combination of serum and tissue samples.Thecombined results led to the discovery of potential protein biomarkers. “Proteomicshas relied almost exclusively on evaluating protein as the criteria for findingbiomarkers” (Blonder Josip, Issaq ,Haleem J, Veenstra Timothy D. 2011)Proteomics presentsseveral problems, as Lisandraindicated.By using two differentmethods (isotope labeling and spectral counting) a large number of differences wereobserved, but, it is unknown which one has the greater probability to be a biomarker.This is the hard part about proteomics because right now you may have a lot ofpossible biomarkers but which ones are the ones that will work? That is the bigquestion that scientist have to answer. Another problem with proteomic biomarkers isthat it has to go through certain stages for validation. The stages are discovery,qualification, verification, and finallyvalidation. It is very difficult to get to thevalidation stage, and that is why many scientist stop before validation. This shows ushow investigators needa lot of perseverance to complete the objectives at hand. Weshould also be openedto changes in my objective, I could start with one and finishwith another one, and the important thing is to keep on going.
Reference:Blonder Josip, Issaq ,Haleem J, Veenstra Timothy D. 2011. Proteomic biomarkerdiscovery: It’s more than just mass spectrometry. Electrophoresis. 32:1541–1548