Bio presentation-respiration


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Respiration in Plants

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Bio presentation-respiration

  1. 1. • The energy required for life process is obtained by oxidation.• Only green plants and cyanobacteria can prepare their own food.• They trap light energy convert chemical energy• Chemical energy is stored in the bonds of carbohydrates like glucose, sucrose, starch.• Only cells containing chloroplast carry out photosynthesis.
  2. 2.  Animals are heterotrophic. They obtain food from animals directly or indirectly. Saprophytes like fungi are dependent on dead and decaying matter. Respiration taking place in all types of living cell are called cellular respiration. Or, it is the mechanism of breakdown of food materials within the cell to release energy, and the trapping of this energy for synthesis of ATP.
  3. 3.  The breaking of the C-C bonds of complex compounds through oxidation within the cells, leading to release of considerable amount of energy is called Respiration. The compounds that are oxidised during this process are known as Respiratory Substrates. They may be carbohydrates, fats and proteins.
  4. 4.  During respiration all the energy contained in respiratory substrate is released in a slow step wise reactions controlled by enzymes and it is trapped as chemical energy in the form of ATP. This energy trapped in ATP is utilised in various energy requiring process of organisms. The carbon skeleton produced during the respiration is used as precursors for biosynthesis of other molecules in the cell.
  5. 5.  Plants have stomata and lenticels for gaseous exchange. There are several reasons why plants can get along without respiratory organs. First, each plant part take care of its own gas exchange needs. Second, plants do not present great demands for exchange. Only during photosynthesis are large volumes of gases exchanged and each leaf is well adapted to take care of its own needs.
  6. 6.  Third, the distance that gas must diffuse even in large bulky plant is not great. During the process of respiration complete combustion of glucose with the help of oxygen produces CO2 and H2O as end products, yields energy most of which is given out as heat. C6H12 O6 + 6O2 6CO2 + 6H2O + Energy
  7. 7.  It is originated from the Greek words, glycos for sugar, and lysis for splitting. The scheme of glycolysis was discovered by 3 German Scientists, Gustav Embden, Otto Meyerhof and J. Parnas, and therefore, reffered as EMP Pathway . Glycolysis is common to both aerobic and anaerobic modes of respiration This is the only process in respiration in anaerobic organisms. Glycolysis occurs in cytoplasm of cells.
  8. 8.  1.Phosphorylation of sugar: Glucose and fructose are phosphorylated to give rise to glucose-6-phosphate and fructose-6-phosphate respectively, by the activity of enzyme hexokinase, in the presence of ATP. Glucose (6 C) + ATP Mg2+hexokinase Glucose-6-phosphate+ADP Now isomerisation occurs: Glucose-6-phosphate Fructose-6-phosphate
  9. 9.  2.Phosphorylation of fructose-6-phosphate:It is phosphorylated and fructose-1, 6-bisphosphate by the action of enzyme phosphofructokinase in pressence of ATP. Fructose-6-phosphate + ATP phosphofrucktokinasemg2+ Fructose-1, 6-biphosphate + ADP 3.Splitting:Fructose-1, 6-biphosphate aldolase 3-phosphoglyceraldehyde(PGAL) + dihydroxyacetone phosphate (Di HAP)
  10. 10.  4.Oxidative dehydrogenation:PGAL + NAD dehydrogenase glyceraldehyde phosphate 1,3- bisphosphoglycerate +NADH + H+ 5.Formation of ATP:1,3-bisphosphoglycerate + ADP phosphoglycerate kinase 3- phosphoglycerate +ATP6.Isomerisation:3-phosphoglycerate 2-phosphoglycerate
  11. 11.  7.Dehydration:2-phosphoglyceratephosphoenol pyruate+ATP2-phosphoenol pyruatepyruvic acid + 2 ATP
  12. 12. Main step of EMP pathway
  13. 13.  It is defined as the anaerobic breakdown of carbohydrates and other organic compounds into alcohol, organic acids etc. 2 types of fermentation are common: 1. Alcoholic fermentation: in this type, pyruvic acid is first decarboxylated to acetaldehyde then to ethanol C6 H12 O6 + 2 CH 3CH2 OH +2 CO2
  14. 14.  2.Lactic acid fermentation: here,pyruvic acid is converted into lactic acid by enzyme lactic dehydrogenase. C6 H12 O6 2 CH 3CHOH.COOH
  15. 15. Pathway of fermentation in yeast
  16. 16.  Pyruate is transported from the cytoplasm into the mitochondria. The crucial events in aerobic respiration are: The complete oxidation of pyruate by the stepwise removal of all the hydrogen atoms, leaving 3 molecules of CO2. The passing on of the electrons removed as part of the hydrogen atoms to molecular O2 with simultaneous synthesis of ATP.
  17. 17.  The first process takes place in the matrix of the mitochondria while the second process is located on the inner membrane of the mitochondria. Pyruvate undergoes oxidative decarboxylation by a complex set of reactions catalysed by pyruvic dehydr ogenase. Pyruvic acid + CoA + NAD+ Acetyl CoA +CO2 + NADH + H+ during this process, 2 molecules of NADH are produced from the metabolism of 2 molecules of pyruvic acid.
  18. 18.  Sir Hans Adolf Krebs in 1937 discovered tricarboxylic acid or citric acid cycle or Krebs cycle. It occurs in the matrix of mitochondria. The starting point of Krebs cycle is entrance of acetyl CoA into a reaction to form citric acid.
  19. 19.  Acetyl CoA + oxaloacetic citric acid + CoA Citric acid Cis-aconitic acid +H2 O Cis-aconitic acid+H2 O Isocitric acid Iso-citric acid + NAD+ Oxalosuccinic acid +NADH +H+ Oxalosuccinic acid -Ketoglutaric acid + CO2 -Ketaglutaric acid + CoA +NAD+ Succinyl CoA + NADH +H+ +CO2 Succinyl CoA +H2 O +GDP+ Ip Succinic acid + CoA + GTP
  20. 20.  GTP+ ADP GDP+ ATP Succinic acid + FAD Fumaric acid+ FADH2 Fumeric acid + H2 O Malic acid Malic acid +NAD+ Oxaloacetic acid +NADH+H+ Pyruvic acid +4NAD+ +FAD + 2H2 O+ADP+Ip mitochondrial matrix 3CO2 + 4NADH +H+ +FADH2 +ATP
  21. 21.  The metabolic pathway through which electron passes from one carrier to another is called electron transport system. It is present in the inner mitochondrial membrane.
  22. 22.  COMPLEX 1:Electrons to produced are oxidised by an NADH dehydrogenase and electrons are transferred to ubiquinone located within the inner membrane. COMPLEX 2: Ubiquinone also recieves reducing equivalents via FADH2 that is generated during oxidation of succinate in the citric acid cycle. COMPLEX 3: The reduced ubiquinone is hen oxidised with the transfer of electrons to cytochrome c via cytochrome bc1 .
  23. 23.  COMPLEX 4:Cytochrome c oxidase complex containing cytochromes a and a3 and 2 copper centres. Complex 5: When the electrons pass from complex 1 to 4, they are coupled to ATP synthase for the production of ATP fro ADP.
  24. 24. RESPIRATORY QUOTIENT It is the ratio of the volume of CO2 evolved to the volume of O2 consumed in respiration RQ =volume of CO2 evolved volume of O2 consumed