Why study the rare biosphere?Produce estimates of total number of taxa in samplesAnswer whether “everything is everywhere.”Enable testing ecological mechanisms for subsistenceEnable research into genes with novel applications Reveal phylogenetic novelty, undetected due to previous methodological limitations Carlos Pedrós-Alió Josh Neufeld
HypothesisRare 16S rRNA genesequences arephylogeneticallynovel and representpersistent low abundancepopulations.
ConclusionsSSUnique and AutoQIIME eﬀective for exploring noveltaxonomic diversityPhylogenetic analysis enabled by complementingnext-gen data with targeted gene/genome retrievalUL5 (BRC1, rare in multiple soils)UL9 (sister to Cyanobacteria and chloroplasts)UL13 (divergent mitochondria of unknown eukaryotes)
Next stepsFUNCTION: targeted genome retrievalDIVERSITY: explore additional environmentsECOLOGY: indicator species analysis
Additional sample datasets New graduate student (you?)
University of Waterloo Michael Lynch (PDF) Andrea Bartram (PhD) Andre Masella (RA) Michael Hall (RA) Jennifer Stearns (PDF) Katja Engel (RA) University of Toronto Dennis Cvitkovitch Ken Croitoru Howie Tenenbaum Michael GoldbergWilfrid Laurier UniversityGabriel Moreno-Hagelsieb