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Embryo Transfer in Alpacas


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Embryo transfer in alpacas

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Embryo Transfer in Alpacas

  1. 1. Basic Concepts About ET in Alpacas Jorge Reyna BSc (Hons), MScVetSc (Sydney Univ.) Alpaca Breeding Technologies
  2. 2. What is embryo transfer? Donor Recipients
  3. 3. ET steps <ul><li>Herd evaluation (donors and recipients) </li></ul><ul><li>Superovulation and mating of donors </li></ul><ul><li>Flushing </li></ul><ul><li>Isolation and evaluation of embryos </li></ul><ul><li>Recipient synchronisation </li></ul><ul><li>Embryo transfer </li></ul>
  4. 4. Herd evaluation <ul><li>Four essential criteria: </li></ul><ul><li>1. Rectal accessibility </li></ul><ul><li>2. Reproductive tract </li></ul><ul><li>3. Ovarian examinations </li></ul><ul><li>4. Cervical accessibility </li></ul><ul><li>HE is essential due to: </li></ul><ul><li>Investment in hormones (2) Investment in laboratory materials </li></ul><ul><li>(3) Time-consuming nature of ET (4) Service fees </li></ul>
  5. 5. Superovulation and Mating of Donors <ul><li>Growth stimulation of several follicles at a time </li></ul><ul><li>Ovulation induction of stimulated follicles </li></ul><ul><li>Mating in order to fertilise the oocytes </li></ul>
  6. 6. How to superovulate? <ul><li>To induce follicular growth multiple doses of FSH are required, 3-5 days 12 hr interval. </li></ul><ul><li>To induce ovulation of these follicles a LH injection is required (GnRH). </li></ul><ul><li>After treatment a luteolytic dose of PGF2 α is required. </li></ul>
  7. 7. Recipient synchronisation <ul><li>Reproductive status must be synchronised between donors and recipients. For that, a single injection of GnRH is used </li></ul>
  8. 8. Flushing of embryos
  9. 9. Embryo assessment
  10. 10. Embryo transfer
  11. 11. Recommendations <ul><li>Minimise stress in the herd before, during and after ET. </li></ul><ul><li>Make sure animals are in a good body condition and healthy. </li></ul><ul><li>Donors and recipients must be animals of proven fertility. </li></ul><ul><li>Two to four weeks before starting a superovulatory treatment is important to supplement animals with lupin seeds. </li></ul>
  12. 12. Contact us <ul><li>Jorge Reyna </li></ul><ul><li>BSc (Hons), MScVetSc (Sydney Univ.) </li></ul><ul><li>2/122 Newington Rd, Petersham, NSW 2049 Australia </li></ul><ul><li>Phone: +61 2 9568 1370, </li></ul><ul><li>Mobile: +61 428 ALPACA </li></ul><ul><li>E-mail: [email_address] </li></ul>
  13. 13. <ul><li>Copyrights ®ArtMinds Digital Media Solutions </li></ul>