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Reseach proposal escrito

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Reseach proposal escrito

  1. 1. University of Puerto Rico at Cayey, Biology Department Research Proposal John Edward Muñoz Torres 804-12-5027I. Title: Effect Of Active Ingredients Of Solanumtorvum (Devil’s Fig) For The Treatment Of Malignant Melanoma CellsII. Abstract: Malignant melanoma is a possibly fatal disease if not diagnosed in time. Treatment for this cancer includes removal of the tumor and chemotherapy. The Solanumtorvumis a plant that grows rapidly that is known to have healing properties on diverse skin diseases and treatment of diabetes. Is there a way to treat malignant melanoma with diverse extracts of Solanumtorvum? By producing multiple extracts using fresh plant components(the roots, leaves, and fruit), since dry components lose their essential chemical base, we can find an active ingredient capable of replacing the chemotherapy used for treatment reducing the mayor side effects patients face when exposed. A technique known as Mosaic Blue Assay will be used since it determines the possibility of effectiveness on a person in the controlled environment of in vitro.III. Introduction: Solanumtorvum(Devil’s fig) is a highly toxic fruit when consumed in high quantities.This plant is green when fresh and turns a yellow color when ripe. The shrub is usually two to three meters in height with a base diameter of five centimeters. This fruit is known to lower blood pressure, and good for the treatment of diabetes. Another function of the fruit in Yucatan is for the treatment of diverse skin diseases. Many different cultures have their use for this plant that is found in most tropical climates including Hawaii, Florida, Mexico, and Puerto Rico. Devil’s fig with this trait could be used for the treatment of epithelial skin diseases, such as malignant melanoma cancer.
  2. 2. IV. Methodology Solanumtorvum is being used for this experiment due to its capacity to reproduce at high speeds in great quantities. This plant is also known to have many medicinal properties in different countries. There will be multiple extracts of the plant using the roots, leaves and fruit in a fresh state, if dried it loses most of its chemical base not permitting for a controlled experiment. Mosaic Blue Assay is a procedure that allows for one to have multiple cultures that are divided mechanically into designated regions in the soft agar, with different experimental groups in a controlled environment the extracts are added and cultivated over a period of 4 days. The Blue dye is added to determine the quantity of cells that survived to the extract allowing for easier identification. This procedure also verifies if the treatment used on the cells will be effective on the patient. In case the extract does not stop or reduce the growth of the malignant melanoma cells the experiment will have less than 1 percent of success on the patient.V. Results: After the completion of the four days, the growth of the cells will be measured and the different extracts compared to see which one was more productive in the reduction of growth on the malignant melanoma cells.VI. References: Mosaic Laboratories [internet]. [2010] Lake Forest (CA): Mosaic Laboratories; [cited 2012 July 5] Available in: http://www.mosaiclabs.com/pics/tests/blue-array.jpg Philippines medicinal plants [internet]. [2012] Philippines medicinal plants; [cited 2012 July 5] Available in: http://www.stuartxchange.com/TandangAso.html American Cancer Society [internet]. [2012] American Cancer Society [cited 2012 July 6] Available in: http://www.cancer.org/Cancer/CancerCauses/SunandUVExposure/skin-cancer-facts MedicineNet.com [internet]. [N/A] MedicineNet.com [cited 2012 July 6] Available in: http://www.medicinenet.com/melanoma/article.htm United States Department of Agriculture [internet]. [N/A] United Stated Department of Agriculture [cited 2012 July 7] Available in: http://plants.usda.gov/java/profile?symbol=SOTO4

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