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Proposal number 4

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Proposal number 4

  1. 1. Presentation 4 Effect Of Active Ingredients Of Solanum torvum (Devil’s Fig) For The Treatment Of Malignant Melanoma Cells By: John Muñoz
  2. 2. What is Solanum torvum?• A plant with a height of two to three meters with a base of five centimeters.• A shrub usually found in the tropics. Highly toxic and destructive weed when mature. On the other hand, the immature fruit is used for consumption in different countries.• Other uses of the plant are for treatment of diabetes and diverse skin diseases.
  3. 3. What is epithelial cancer?• Epithelial cancer is a dangerous disease if not detected in time, and treated, could possibly lead to death. There are three types of skin cancers: – Basal carcinoma cancer: which starts in the base cells of the epidermis and moves down. – Squamous cell carcinoma: affecting the squamous cells (top cells of the epidermis). – Malignant melanoma: is the third type of epithelial cancer and the most dangerous since it can spread through the body of the individual, usually taking root in overgrown moles.
  4. 4. Problem• Is there an Active ingredient in the extract of Solanum torvum for the treatment of Malignant melanoma cancer cells?
  5. 5. Hypothesis• The diverse active ingredients in the plant extract of the Solanum torvum, lead for the future treatment of Malignant melanoma without exposing the patient to severe side effects.
  6. 6. Methodology• The chemical base of the Solanum torvum will be revised for the possible active ingredients of the plant.• The extract will be made of all components of the specimen: leaves, roots, and fruit (immature and ripe).• Using the extracts I will observe quantitatively, in vitro, the effect of them in the cancer cells’ growth. Using the method of Mosaic Blue Assay.• My control group will be a group of Malignant melanoma cells growing without the addition of the extracts.• The experimental group will be Malignant melanoma cells growing in the Mosaic Blue Assay treated with the extracts of each extract.• Analyze the results in the growth of the cells
  7. 7. Analyze the results• I will be checking the growth of the cells with a florescent plate reader that activates with the dye used in the Mosaic Blue Array.• Then I will compare the different components of the plant. Which extract worked best and why and the active ingredient of the extract.
  8. 8. Picture from: http://www.mosaiclabs.com/test_invitro
  9. 9. ReferenceMosaic Laboratories [internet]. [2010] Lake Forest (CA):Mosaic Laboratories; [cited 2012 July 5] Available in:http://www.mosaiclabs.com/pics/tests/blue-array.jpgPhilippines medicinal plants [internet]. [2012]Philippines medicinal plants; [cited 2012 July 5] Available in:http://www.stuartxchange.com/TandangAso.htmlAmerican Cancer Society [internet]. [2012]American Cancer Society [cited 2012 July 6] Available in:http://www.cancer.org/Cancer/CancerCauses/SunandUVExposure/skin-cancer-factsMedicineNet.com [internet]. [N/A]MedicineNet.com [cited 2012 July 6] Available in:http://www.medicinenet.com/melanoma/article.htmUnited States Department of Agriculture [internet]. [N/A]United Stated Department of Agriculture [cited 2012 July 7] Available in:http://plants.usda.gov/java/profile?symbol=SOTO4
  10. 10. QUESTIONS?

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