The Sanitary Quality of Pond Water and Canal Water of UPR in Cayey P.R. Campus<br />JESYKA MELÉNDEZ¹, WILMER FELICIANO¹ & ...
Specific Aims<br />To provide a general overview about the water quality from selected areas of the UPR in Cayey campus.<b...
Hypothesis<br />Because of the presence of animals in the campus we believe there will be a highly significant amount of c...
Background and Significance<br />Water quality studies are extremely important because these bacteria “do not change the a...
Background and Significance<br />The possible contamination of these water bodies could be an indicator of contamination i...
Research Design and Methods<br />Water samples are to be recovered from ten different locations along the water pond and c...
Google earth<br />
Water Sample Locations<br />
Research Design and Methods<br />Colilert was added to the samples.<br />The samples were placed in trays and incubated.<b...
Research Design and Methods<br />To ensure the presence of E. coli, a PCR was conducted with extracted bacterial DNA.<br />
Results<br />The samples were corroborated for the presence of total coliforms by examining each of the 48 tray cells for ...
Coliform and E. coli Presence<br />
Numerical Probability Table<br />
PCR Results<br />It is evident by the thickness and clarity of all the bands that E. coli is present in all ten samples of...
PCR Results<br />
Acknowledgements<br />Belinda Roman PhD<br />We wish to extend a special thanks to the RISE program and its entire staff f...
Literature Cited & References<br />Anderson, K. and Davidson, M. Drinking Water & Recreational Water Quality: Microbiologi...
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The Sanitary Quality of Pond Water and Canal Water of UPR in Cayey P.R. Campus

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The Sanitary Quality of Pond Water and Canal Water of UPR in Cayey P.R. Campus

  1. 1. The Sanitary Quality of Pond Water and Canal Water of UPR in Cayey P.R. Campus<br />JESYKA MELÉNDEZ¹, WILMER FELICIANO¹ & BELINDA ROMÁN² PhD, <br />RISE program UPR Cayey P.R. Grant number 5 R25 GM059429<br />¹Undergraduate research students at University of P.R. in Cayey, Department of Biology. ² Mentor in charge of investigation. PhD professor at University of P.R. in Cayey Biology Department.<br />
  2. 2. Specific Aims<br />To provide a general overview about the water quality from selected areas of the UPR in Cayey campus.<br />
  3. 3. Hypothesis<br />Because of the presence of animals in the campus we believe there will be a highly significant amount of coliforms present in the water bodies. <br />
  4. 4. Background and Significance<br />Water quality studies are extremely important because these bacteria “do not change the appearance or taste of water there, is no way to tell if these microorganisms are present”.<br />These types of bacteria may cause side effects such as; diarrhea, cramps, headaches and other symptoms.<br />
  5. 5. Background and Significance<br />The possible contamination of these water bodies could be an indicator of contamination in other nearby water bodies. <br />E. coli is extremely reliable as a fecal contamination indicator in water.<br />Because the presence of E. coli in the water indicates the possible contamination of the water with pathogenic organisms such as Giardialamblia and Cryptosporidium parvum, it is important to conduct further experiments in the case that the water samples are contaminated. <br />
  6. 6. Research Design and Methods<br />Water samples are to be recovered from ten different locations along the water pond and canal.<br />
  7. 7. Google earth<br />
  8. 8. Water Sample Locations<br />
  9. 9. Research Design and Methods<br />Colilert was added to the samples.<br />The samples were placed in trays and incubated.<br />The test trays are then removed from the incubator and the yellow cells, which would indicate the presence of total coliforms, are counted. <br />The test trays are exposed to a UV lamp and the florescent cells, which indicate the E. coli presence, are noted. <br />
  10. 10. Research Design and Methods<br />To ensure the presence of E. coli, a PCR was conducted with extracted bacterial DNA.<br />
  11. 11. Results<br />The samples were corroborated for the presence of total coliforms by examining each of the 48 tray cells for yellow cells. <br />The trays were then examined with UV <br /> light for fluorescing <br /> E. coli containing cells. <br />http://sanctuarymonitoring.org/regional_images/monitoring_projects/100241_image1.jpg<br />
  12. 12. Coliform and E. coli Presence<br />
  13. 13. Numerical Probability Table<br />
  14. 14. PCR Results<br />It is evident by the thickness and clarity of all the bands that E. coli is present in all ten samples of water taken from the UPR in Cayey Campus. The PCR confirms the previously acquired information by incubation of the test trays containing the colilert.<br />
  15. 15. PCR Results<br />
  16. 16. Acknowledgements<br />Belinda Roman PhD<br />We wish to extend a special thanks to the RISE program and its entire staff for their always enthusiastic support and guidance.<br />
  17. 17. Literature Cited & References<br />Anderson, K. and Davidson, M. Drinking Water & Recreational Water Quality: Microbiological Criteria University of Idaho, Cooperative Extension System of Agriculture Experiment Station<br />Deng M., Fratamico P., Jensen M., Tice G. 1996. DNA Amplification using Tableted PCR Reagents for Identification of Escherichia coli 0157:H7 Isolated from Foods. Journal of Rapid Methods and Automation in Microbiology 5(1):61-74<br />Gunzburg S., Tornieporth N., Riley L. 1995. Identification of Enteropathogenic Escherichia coli by PCR Based Detection of the Bundle-Forming Pilus Gene. American Society of Microbiology 33(5):1375-1377<br />LeChevallier M., Norton W., Lee R., 1991. Giardiaand Cryptosporidium spp. in Filtered Drinking Water Supplies. American Society for Microbiology 57(9):2617-2621 <br />Skipton, S., Dvorak, B., Woldt, W., Wirth, S. 2008 Drinking Water Bacteria DHHS Nebraska<br />Well Stewardship Information Series 2002 Total and Faecal Coliform Bacteria in Groundwater<br />EDVO-Kit # 948. The Biotechnology Education Company®<br />BACKGROUND: http://www.mainsgate.com/spacebio/modules/mb_resource/Ecoli.jpeg<br />

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