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  1. 1. FibromyalgiaHow Does The Pain Start ? <br />Jeremy Ríos Segarra<br />Dr. Edwin Vazquez<br />Biol 1300<br />University of Puerto Rico at Cayey<br />
  2. 2. Fibromyalgia<br />Fibromyalgia is a disorder characterized by widespread musculoskeletal pain accompanied by fatigue, sleep, memory and mood issues. Researchers believe that fibromyalgia amplifies painful sensations by affecting the way your brain processes pain signals. <br />
  3. 3. Fibromyalgia<br />It has been called a “stress-related disorder” due to the fact that its symptoms starts and/or worsen occur in the context of stressor events.<br />
  4. 4. Objective<br />Determine if the ApolipoproteinE (APOE) is the cause of the susceptibility that the siblings are affected with.<br />
  5. 5. Hypothesis <br />The APOE is the factor that siblings from parents with Fibromyalgia are inheriting.<br />
  6. 6. Specific aims<br />Gather blood samples with and without Fibromyalgia.<br /> Amplified directly from whole blood using Finnzymes ́ Phusion® Blood Direct PCR Kit. <br />Digest with restriction enzymes. <br />Run agarose gel electrophoresis.<br />
  7. 7. Methodology<br />From 20 to 40 adult female individuals age 47 to 59, with a clinical diagnosis of fibromyalgia.<br />Control sample consists from 20 to 40 adult female individuals from age 47 to 59<br />
  8. 8. Methodology<br />Blood samples from both groups will be extracted and we will be using PCR/RFLP technique to identify the APOE gene.<br />
  9. 9. PCR/RFLP<br />A Restriction Fragment Length is a variation in the DNA sequence of a genome which can be detected by a laboratory technique known as gel electrophoresis. Analysis of RFLP variation was an important tool in genome mapping, localization of genetic disease genes, determination of risk for a disease, genetic fingerprinting, and paternity testing.<br />
  10. 10. Expected Results<br />The APOE is presented on the siblings<br />This gene is the one making the sibling susceptible to the Fibromyalgia.<br />
  11. 11. References <br />Mayo Clinic staff, Fibromyalgia, Mayo Foundation for Medical Education and Research (MFMER), Jan. 22, 2011.<br />William C. Shiel Jr., Fibromyalgia,MedicineNet, Inc,June 30, 2011.<br />Connie Luedtke, R.N., Is fibromyalgia hereditary?,April 9, 2011.<br />Fibromyalgia, A.D.A.M, February 14, 2011.<br />Fibromyalgia,  Ideal Health Services Pty Ltd.,<br />PCR-RFLP directly from blood using Phusion® Blood Direct PCR KitPCR-RFLP directly from blood using Phusion® Blood Direct PCR Kit,ArktikTM and Phusion®,<br />