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The 5 Second Rule

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By: Blake, Peyton, and Cassidy

Published in: Education
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The 5 Second Rule

  1. 1. The 5 Second Rule By: Blake, Peyton, and Cassidy
  2. 2. Question Which type of food will grow the most bacteria after being dropped on the ground for 5 seconds?
  3. 3. We found our research at www.education.com I went onto Google.com and looked up experiments clicked on the link to Education.com and found the five second rule.
  4. 4. Hypothesis Bacteria will spread on the piece of food and contaminate it. Not so much that you can’t eat it but will spread bacteria.
  5. 5. Materials Materials: ● Food items to be tested (e.g., wet item like lunch meat and dry item like a jelly bean). You will need six of each item tested. ● Sterile swabs ● Sterile gloves ● Timer ● Prepared nutrient agar in sterile plates ● Notebook to record results
  6. 6. procedures Experimental Procedure 1. Prepare 12 sterile plates containing nutrient agar. 2. Put on sterile gloves (do not touch any non-sterile surface when wearing gloves). 3. Select two types of “ground." 4. Drop the first test item (e.g., a piece of lunch meat) on the first type of ground. 5. Start the timer. 6. Remove the item from the ground after five seconds. 7. Swab the item with a sterile swab (do not touch anything else with the swab). 8. Remove the top of the petri dish. Keep the top of the dish in your hand away from any non-sterile surface. 9. Gently run the swab back and forth in a zigzag pattern on the surface of the agar plate. Do not touch any part of the agar twice. 10. Put the top of the petri dish back on and label. 11. Change your gloves. 12. Repeat the test with the first item (e.g., a new piece of lunch meat). 13. Repeat the test twice with the second item (e.g., two separate jelly beans). 14. Change types of ground and perform the test twice with each item. 15. Swab items that have not been dropped on any ground. Create 2 “control” agar plates for each item tested. 16. Place the petri dishes in an environment that is as close to 37ºC as possible. Bacteria will take longer to grow at room temperature. Ensure that all petri dishes are placed in the same location.
  7. 7. Results Our experiment was a success. It was a cracker that had the most bacteria. We thought that the cheese would have grown the most. The experiment was fun to do.
  8. 8. Conclusion In conclusion this is a great experiment that any 5th grader would be happy to do. It does get a bit gross but is also super cool. When you see what has grown on the foods you will be surprised on how you might have thought that the most bacteria would grow on one food and may grow on another. So, this experiment was a total success and we suggest you to try it out yourself.
  9. 9. Pictures EEEWWWWWWWW :( ;(
  10. 10. 1.Which mouth has more bacteria a Mini Rex rabbit, a bearded dragon lizard, Sulcata tortoise or a human? 2. We found our sources by Mrs. Abernathy. 3. We think that Thunder will have the most bacteria in his mouth. 4. Sterile gloves, Sterile glove and Prepared nutrient agar in sterile plates 5. Take 4 cotton swabs and place them in each of the mouths. Prepare the nutrient agar in sterile plates.
  11. 11. Animal v. Human continued. 5.After you have swabbed the mouths place each of the cotton swabs into a petri dish and keep in a cool dark spot for 5-9 days. 6. The results are that Thurman the Sulcata tortoise had the most bacteria on his swab which surprised us all. 7. In conclusion we were so so surprised that Thurman had the most bacteria in his mouth. We are very glad that we did this experiment.
  12. 12. The End Thanks for watching!

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