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Assisted Hatching in Assisted Reproductive Technology-Procedure of Assisted Hatching in
IVF-IVF Success Rates and Assisted Hatching-In vitro success and assisted hatching-IVF
success rate -Increased by Assisted Hatching of embryos-Assisted Hatching in Assisted
Reproduction-IVF and Assisted Hatching
The assisted hatching technique was developed in 1990 by a team led by Dr. Jacques Cohen at
Cornell University in New York. Since then many IVF centres have adopted this. Although there is
some variability in results and technique, most studies on Assisted Hatching have demonstrated that
it improves implantation rates in certain patients undergoing IVF and/or IVF/ICSI.

What Is Assisted Hatching?
Assisted hatching is a laboratory technique designed to enhance implantation of embryos generated
through in vitro fertilization (IVF). Most people are familiar with the failure of a cycle which is an
unfortunate reality of assisted reproduction. Even when presumably high quality, fertile eggs are
used, such as in egg donation with surrogates, nearly 40% to half of couples are still left
disappointed each cycle. Since most couples are able to get eggs and have fertilization, then the
question is where does the failure occur? The answer may be in the implantation phase.

After a fertilized egg is returned to the uterus, several things must happen:
• It must continue to divide and grow (it is now called an embryo).
• The embryo must break out of the zonapellucida (usually called the zona) which is a hard protein
shell that surrounds it.
• The embryo must then burrow into the lining of the uterus (called the endometrium) and continue
to grow there.
Assisted hatching is a laboratory procedure whereby the zona of the early embryo is mechanically or
chemically weakened in a way that assists the embryo to "hatch" from the zona more easily allowing
implantation into the lining of the uterus.It has been suggested that making a hole in or thinning this
outer layer may help embryos to ‘hatch’, increasing the chances of the woman becoming pregnant in
some cases.

For whom is the Assisted Hatching with IVF suitable?
Assisted hatching is thought to be helpful for couples with a poor prognosis whose embryos are
thought to lack sufficient energy to complete the hatching process.
According to the American Society for Reproductive Medicine, assisted hatching may be indicated
for women with advanced maternal age (older than 38), two or more failed IVF cycles and poor
embryo quality. Apart from this Egg quantity and quality factor and Zona factor - cases with embryos
that have a thick outer shell also gives indication for the use of Assisted Hatching Technique.
Because assisted hatching is a difficult technique, the success is dependent on the embryologist's
experience and technique, it is important to talk with your fertility clinic about how successful they
are with the procedure.

Is Assisted Hatching with IVF Safe?
There can be complications from assisted hatching. It is possible to damage embryos with hatching
and lower the pregnancy rates. Therefore, it is essential that if assisted hatching is done, it must be
expertly performed by properly trained embryologists. In addition, assisted hatching has been
associated with an increased risk of monozygotic (identical) twins.
Views remain mixed as to whether the technique actually improves the chances of pregnancy, but
most agree that it may be helpful in selected groups of patients.
Assisted hatching is done while the embryo is in the laboratory. Before being transferred back to the
womb a hole is made in the outer layer of the embryo or it is thinned, using acid, laser or mechanical
methods. Assisted Hatching using laser technology is probably the best technique. Laser assisted
hatching is a gentle and safe way to weaken a part of zonapellucida. Several studies have shown that
using a laser is superior to chemical and manual hatching. Miinimal handling of the embryo and
delivering fast and exact control over the drilling of the hole are the advantages of laser assisted
hatching
Laser assisted hatching is performed prior to the embryos being transferred back to the uterus.
The actual pregnancy and live birth rates seen in an individual IVF center or infertility clinic will vary
according to the hatching technique used, the overall quality of the laboratory, the skill of the
individual performing the hatching, the embryo transfer skills of the physician, and other factors.
Assisted hatching of IVF embryos,is an in vitro fertilization lab procedure that can increase IVF
success rates,,Ask infertility expert at Rotunda Fertility Center for complete medical advice for
infertile couples.
At Rotunda - The Center for Human Reproduction, Laser technique is used to perform the assisted
hatching procedure. The team of doctors and embryologist are well adversed with the latesy
technology in the field of ART(Assisted Reproductive Technology). You can contact the doctors at
Rotunda clinic at http://www.iwannagetpregnant.com or http://www.surrogacymumbai.com/ or at
you can have a look at their Assisted Hatching procedure at
http://www.rotunda.co.in/index.php?option=com_content&id=76&Itemid=55 or simply give a call
on +91 22 2655 2000 / 2640 5000 and they will be ready to help you.
SUMMARY
In vitro fertilization (IVF) failure is a frustrating experience for individuals and couples, and it is often
caused by an embryo's failure to implant in the uterus. For women who have failed IVF or have a
poor prognosis for IVF, the fertility doctor may recommend a technique known as assisted
hatching.Assisted hatching is a laboratory technique designed to enhance implantation of embryos
generated through in vitro fertilization (IVF).

Keywords
Assisted Hatching, assisted hatching success rates, assisted hatching ivf, assisted hatching, in vitro
fertilization, hatching, ivf, shell, zonapellucida, implantation, pregnancy rates, zona, ivf clinics,
infertility clinics, fertility clinics,IVF Success Rates,assisted hatching procedure, assisted hatching
success rate, assisted hatching risks, assisted hatching ivf, assisted hatching success, icsi, ivf,
zonapellucida, laser assisted hatching, endometrium, Assisted Reproductive Technology, assisted
hatching at Rotunda, embryos, failed ivf cycle, female age factor, increased age in female, Fertility,
Infertility, Male Fertility, Female Fertility, Male Infertility, Female Infertility, Pregnancy, PCOS
Infertility, Premature Ovarian Failure, Diagnosis, in vitro fertilization, IVF, natural cycle, embryo,
zonapellucida thinning, hatching, hatch, IVF techinque, Assisted Hatching, assisted hatching success
rates, assisted hatching ivf, assisted hatching, in vitro fertilization, hatching, ivf, shell, zonapellucida,
implantation, pregnancy rates, zona, ivf clinics, infertility clinics, fertility clinics, infertility expert,
LASER, Zona drilling, Tyrode’s solution

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Laser Assisted Hatching | IVF | Fertility Specialists India

  • 1. Assisted Hatching in Assisted Reproductive Technology-Procedure of Assisted Hatching in IVF-IVF Success Rates and Assisted Hatching-In vitro success and assisted hatching-IVF success rate -Increased by Assisted Hatching of embryos-Assisted Hatching in Assisted Reproduction-IVF and Assisted Hatching The assisted hatching technique was developed in 1990 by a team led by Dr. Jacques Cohen at Cornell University in New York. Since then many IVF centres have adopted this. Although there is some variability in results and technique, most studies on Assisted Hatching have demonstrated that it improves implantation rates in certain patients undergoing IVF and/or IVF/ICSI. What Is Assisted Hatching? Assisted hatching is a laboratory technique designed to enhance implantation of embryos generated through in vitro fertilization (IVF). Most people are familiar with the failure of a cycle which is an unfortunate reality of assisted reproduction. Even when presumably high quality, fertile eggs are used, such as in egg donation with surrogates, nearly 40% to half of couples are still left disappointed each cycle. Since most couples are able to get eggs and have fertilization, then the question is where does the failure occur? The answer may be in the implantation phase. After a fertilized egg is returned to the uterus, several things must happen: • It must continue to divide and grow (it is now called an embryo). • The embryo must break out of the zonapellucida (usually called the zona) which is a hard protein shell that surrounds it. • The embryo must then burrow into the lining of the uterus (called the endometrium) and continue to grow there. Assisted hatching is a laboratory procedure whereby the zona of the early embryo is mechanically or chemically weakened in a way that assists the embryo to "hatch" from the zona more easily allowing implantation into the lining of the uterus.It has been suggested that making a hole in or thinning this outer layer may help embryos to ‘hatch’, increasing the chances of the woman becoming pregnant in some cases. For whom is the Assisted Hatching with IVF suitable? Assisted hatching is thought to be helpful for couples with a poor prognosis whose embryos are thought to lack sufficient energy to complete the hatching process. According to the American Society for Reproductive Medicine, assisted hatching may be indicated for women with advanced maternal age (older than 38), two or more failed IVF cycles and poor embryo quality. Apart from this Egg quantity and quality factor and Zona factor - cases with embryos that have a thick outer shell also gives indication for the use of Assisted Hatching Technique.
  • 2. Because assisted hatching is a difficult technique, the success is dependent on the embryologist's experience and technique, it is important to talk with your fertility clinic about how successful they are with the procedure. Is Assisted Hatching with IVF Safe? There can be complications from assisted hatching. It is possible to damage embryos with hatching and lower the pregnancy rates. Therefore, it is essential that if assisted hatching is done, it must be expertly performed by properly trained embryologists. In addition, assisted hatching has been associated with an increased risk of monozygotic (identical) twins. Views remain mixed as to whether the technique actually improves the chances of pregnancy, but most agree that it may be helpful in selected groups of patients. Assisted hatching is done while the embryo is in the laboratory. Before being transferred back to the womb a hole is made in the outer layer of the embryo or it is thinned, using acid, laser or mechanical methods. Assisted Hatching using laser technology is probably the best technique. Laser assisted hatching is a gentle and safe way to weaken a part of zonapellucida. Several studies have shown that using a laser is superior to chemical and manual hatching. Miinimal handling of the embryo and delivering fast and exact control over the drilling of the hole are the advantages of laser assisted hatching Laser assisted hatching is performed prior to the embryos being transferred back to the uterus. The actual pregnancy and live birth rates seen in an individual IVF center or infertility clinic will vary according to the hatching technique used, the overall quality of the laboratory, the skill of the individual performing the hatching, the embryo transfer skills of the physician, and other factors. Assisted hatching of IVF embryos,is an in vitro fertilization lab procedure that can increase IVF success rates,,Ask infertility expert at Rotunda Fertility Center for complete medical advice for infertile couples. At Rotunda - The Center for Human Reproduction, Laser technique is used to perform the assisted hatching procedure. The team of doctors and embryologist are well adversed with the latesy technology in the field of ART(Assisted Reproductive Technology). You can contact the doctors at Rotunda clinic at http://www.iwannagetpregnant.com or http://www.surrogacymumbai.com/ or at you can have a look at their Assisted Hatching procedure at http://www.rotunda.co.in/index.php?option=com_content&id=76&Itemid=55 or simply give a call on +91 22 2655 2000 / 2640 5000 and they will be ready to help you.
  • 3. SUMMARY In vitro fertilization (IVF) failure is a frustrating experience for individuals and couples, and it is often caused by an embryo's failure to implant in the uterus. For women who have failed IVF or have a poor prognosis for IVF, the fertility doctor may recommend a technique known as assisted hatching.Assisted hatching is a laboratory technique designed to enhance implantation of embryos generated through in vitro fertilization (IVF). Keywords Assisted Hatching, assisted hatching success rates, assisted hatching ivf, assisted hatching, in vitro fertilization, hatching, ivf, shell, zonapellucida, implantation, pregnancy rates, zona, ivf clinics, infertility clinics, fertility clinics,IVF Success Rates,assisted hatching procedure, assisted hatching success rate, assisted hatching risks, assisted hatching ivf, assisted hatching success, icsi, ivf, zonapellucida, laser assisted hatching, endometrium, Assisted Reproductive Technology, assisted hatching at Rotunda, embryos, failed ivf cycle, female age factor, increased age in female, Fertility, Infertility, Male Fertility, Female Fertility, Male Infertility, Female Infertility, Pregnancy, PCOS Infertility, Premature Ovarian Failure, Diagnosis, in vitro fertilization, IVF, natural cycle, embryo, zonapellucida thinning, hatching, hatch, IVF techinque, Assisted Hatching, assisted hatching success rates, assisted hatching ivf, assisted hatching, in vitro fertilization, hatching, ivf, shell, zonapellucida, implantation, pregnancy rates, zona, ivf clinics, infertility clinics, fertility clinics, infertility expert, LASER, Zona drilling, Tyrode’s solution