Rapid Detection and Identification of Overdose Drugs in  Saliva by Surface-Enhanced Raman Spectroscopy   Chetan Shende, Fr...
Challenge: Drugs in saliva        National Highway Traffic Safety Administration Stats:        2007: 11% of drivers stoppe...
The Solution: SERSSpecificity: Every chemical produces a unique Raman spectrumallowing unequivocal identification.Sensitiv...
Gold Sol-Gel SERS Capillary                        Providing Chemical Information When & Where You Need It
RTA’s SERSID - Trace Chemical Analyzer                            2010     Patents: 6623977, 6943031, 6943032, 7312088,   ...
UK Road-Side Screening: Required Drugs     35 drugs & metabolites were measured31 active on gold, 4 (barbiturates) active ...
The Proposed DeviceThe proposed SERS-RSSD-DD (Road Side Screening Device for DrugDetection) will extract, identify, and qu...
SERS of Representative Illicit, Prescription & OTC Drugs in Expanded Gold Spectral Library (150) PCP                      ...
Library Search: Measure Priority DrugsSpectra search and identification works for all drugs                               ...
Cocaine: Static Concentration Data                               50 ppb Cocaine                        Background from gla...
Sensitivity: Static ROC Curves of Cocaine                                                    50 ppb      100 ppb          ...
Developed Saliva Extraction Method  1. Collect 0.5 ml in 1 min     2. Expel sample into buffer            3. Filter sample...
50 ppb Drug in Saliva SamplesExtracted from 0.5 mL saliva & detected in 10-minutes!!           Cocaine 50ppb              ...
Cocaine Extracted from Saliva           Saliva ROC Curve: A) 75, B) 50, C) 25 ppbProbability of detection is 66% at 25 ppb...
R&D: SERS Lab-On-ChipDifferent Lab-on-Chip designs used                                     Providing Chemical Information...
Preliminary LOC Results: in Saliva at 50 ppb                                         diazepam 50ppbamphetamine 50ppb      ...
Actual Saliva Measurements: UK Tests                                                          In Water           Results: ...
Real Clinical Saliva Samples     Chronic user, male #1             Casual user, female                                    ...
150 Drugs in    Expanded Gold    SERS Library:Providing Chemical Information When & Where You Need It
Summary: saliva drug analysis    • Non-Invasive: Cotton Swab    • Field Portable: 7 pound Raman analyzer    • Small Sample...
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Pittcon 2012 Cs Fi Invited Sers Talks Drugs In Saliva

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Pittcon 2012 Cs Fi Invited Sers Talks Drugs In Saliva

  1. 1. Rapid Detection and Identification of Overdose Drugs in Saliva by Surface-Enhanced Raman Spectroscopy Chetan Shende, Frank E. Inscore, Atanu Sengupta, Hermes Huang and Stuart Farquharson Screening Abused Drugs in Saliva at Road-Side & Emergency-Room for Driver Impairment & Overdose Funding: UK Road Policing Technologies Home Office Scientific Development Branch NIH and NSFwww.rta.biz Pittcon 2012 RTA Booth #2110chetan@rta.biz Providing Chemical Information When & Where You Need It
  2. 2. Challenge: Drugs in saliva National Highway Traffic Safety Administration Stats: 2007: 11% of drivers stopped tested positive for drugs 2009: 18% of driver fatalities tested positive for drugsThe analyzer must typically provide the following: • Specificity – Identify and discriminate drugs (No False Positives!) • Sensitivity – Detect ~10-8 M or less (10-50 ppb) • Reproducibility – Accurate and Repeatable (No False Negatives!) • Speed – Analysis within 10 minutes • Field Usable – Battery Operated and Rugged
  3. 3. The Solution: SERSSpecificity: Every chemical produces a unique Raman spectrumallowing unequivocal identification.Sensitivity: Silver and gold nanoparticles increase Raman signals by1 million times or more allowing 10-8M (ppb) detection. Raman: Pure Cocaine CH3 + H Cl - N CO2CH3 SERS: O2 C 100 ppb Cocaine Cocaine Hydrochloride
  4. 4. Gold Sol-Gel SERS Capillary Providing Chemical Information When & Where You Need It
  5. 5. RTA’s SERSID - Trace Chemical Analyzer 2010 Patents: 6623977, 6943031, 6943032, 7312088, 7393691, 7393692, 7462492, 7462493, 7713914
  6. 6. UK Road-Side Screening: Required Drugs 35 drugs & metabolites were measured31 active on gold, 4 (barbiturates) active on silver Spectra search worked for all drugs Providing Chemical Information When & Where You Need It
  7. 7. The Proposed DeviceThe proposed SERS-RSSD-DD (Road Side Screening Device for DrugDetection) will extract, identify, and quantify the presence of drugs (and metabolites) in driver saliva at ~10-8M within 8-10 minutes. 6 Providing Chemical Information When & Where You Need It
  8. 8. SERS of Representative Illicit, Prescription & OTC Drugs in Expanded Gold Spectral Library (150) PCP Diazepam Methamphetamine Ritalin MDMA Demerol LSD Hydrocodone Heroin Oxycodone Acetaminophen Acetylsalicylic acid Ibuprofen Providing Chemical Information When & Where You Need It
  9. 9. Library Search: Measure Priority DrugsSpectra search and identification works for all drugs Hit Quality Name 1 0.001 Nordiazepam 2 0.010 Methadone 3 0.010 Oxazepam 4 0.010 Temazepam 5 0.012 Norcodeine Hit Quality Name 1 0.036 Nordiazepam 2 0.357 Temazepam 3 0.363 Diazepam 4 0.373 Methadone 5 0.392 Oxazepam Providing Chemical Information When & Where You Need It
  10. 10. Cocaine: Static Concentration Data 50 ppb Cocaine Background from glass capillary25 ppb = 7x10-8M It can be subtracted. Providing Chemical Information When & Where You Need It
  11. 11. Sensitivity: Static ROC Curves of Cocaine 50 ppb 100 ppb 25 ppb 75 ppb 50 ppb 25 ppb 0 ppb• ROC = establish threshold that signal can be detected above noise.• K value = statistically significant separation between true & false response.• K > 3.29 used by US Army for 95% probability of detection.• Sensitivity = 48 ppb. Indicates that Cocaine SERS peaks can be positively detected above noise 95% of the time at 48 ppb and higher. Providing Chemical Information When & Where You Need It
  12. 12. Developed Saliva Extraction Method 1. Collect 0.5 ml in 1 min 2. Expel sample into buffer 3. Filter sample4. Draw sample into microSPE 5. Elute cocaine from SPE 6. Inject sample in SERS Capillary 7. Measure spectrum Capillary microSPE used for extraction Providing Chemical Information When & Where You Need It
  13. 13. 50 ppb Drug in Saliva SamplesExtracted from 0.5 mL saliva & detected in 10-minutes!! Cocaine 50ppb Hit Quality Name Diazepam 50ppb 1 0.236 Cocaine 1 0.171 Diazepam PCP 50ppb 1 0.171 PCP Methadone 50ppb 1 0.066 Methadone Providing Chemical Information When & Where You Need It
  14. 14. Cocaine Extracted from Saliva Saliva ROC Curve: A) 75, B) 50, C) 25 ppbProbability of detection is 66% at 25 ppb, 92 % at 50ppb and100% at 75 ppb, at 95% confidence level. Providing Chemical Information When & Where You Need It
  15. 15. R&D: SERS Lab-On-ChipDifferent Lab-on-Chip designs used Providing Chemical Information When & Where You Need It
  16. 16. Preliminary LOC Results: in Saliva at 50 ppb diazepam 50ppbamphetamine 50ppb Library referencecocaine 50ppb PCP 50ppb methadone 50ppb All Drugs Correctly Identified by Spectral Match! Providing Chemical Information When & Where You Need It
  17. 17. Actual Saliva Measurements: UK Tests In Water Results: 50 ppb CocaineSpectra of samples measured by UK personnel 1 ppm PCP Note: Cocaine, Methamphetamine, & PCPspectra are very similar, but sufficiently different to be identified. In Saliva In Saliva 50 ppb PCP 100 ppb Cocaine 100 ppb Metham- Male Saliva phetamine Female 50 ppb PCP Saliva
  18. 18. Real Clinical Saliva Samples Chronic user, male #1 Casual user, female Sample collected 30 minutes after use male #2 5 hours after useAll Samples Correctly Identified by Spectral Match! Providing Chemical Information When & Where You Need It
  19. 19. 150 Drugs in Expanded Gold SERS Library:Providing Chemical Information When & Where You Need It
  20. 20. Summary: saliva drug analysis • Non-Invasive: Cotton Swab • Field Portable: 7 pound Raman analyzer • Small Sample Volume: < 1 mL • Rapid & Simple Analysis: 10 minutes (total) • Specificity: similar drugs can be differentiated • Generality: over 250 drugs measured • Sensitivity: measured < 50 ppb in saliva References: 1) Detection of Drugs of Abuse in Saliva by Surface-enhanced Raman spectroscopy. Applied Spectroscopy, 65, 9 (2011). 2) Rapid Detection and Identification of Overdose Drugs in Saliva by Surface- enhanced Raman Scattering Using Fused Gold Colloids. Pharmaceutics, 3, 425-439 (2011).www.RTA.biz Booth 2110 Providing Chemical Information When & Where You Need It

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