Write in the following in your notebook  <ul><li>Title: The Properties of Enzymes: A Study of Catalase </li></ul><ul><li>P...
Enzyme-Substrate Complex
Denaturing Enzymes x x
What is Catalase <ul><li>Enzyme that breaks down hydrogen peroxide (H 2 O 2 ), which is a naturally occurring poison. </li...
Complete this sentence… <ul><li>The _____________ (more/less) enzyme, the faster the product is made. </li></ul>
The Procedure <ul><li>Part A: </li></ul><ul><li>Label 3 large test tubes hot, cold and room temp.  </li></ul><ul><li>Cut 3...
<ul><li>6. Add 1ml of distilled water to each test tube containing  potato  mash.  </li></ul><ul><li>7. Place the test tub...
<ul><li>11.  Add 5ml of H 2 O 2  (3.0% hydrogen peroxide) to each tube. </li></ul><ul><li>12. Wait one minute while reacti...
Teacher Role  Student Role <ul><li>Safety </li></ul><ul><li>Read procedure to know what to do next </li></ul><ul><li>Follo...
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Potato catalse lab

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Lab procedure for investigating the effect of temperature on enzymes.

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  • Adding heat or changing pH denatures enzymes so they don’t work.
  • Potato catalse lab

    1. 1. Write in the following in your notebook <ul><li>Title: The Properties of Enzymes: A Study of Catalase </li></ul><ul><li>Purpose: Potato catalase and hydrogen peroxide will be used to explore the relationship between enzymes and substrates at various temperatures (maybe pH too). </li></ul><ul><li>Procedures: You are going to write them down. Yay! </li></ul>
    2. 2. Enzyme-Substrate Complex
    3. 3. Denaturing Enzymes x x
    4. 4. What is Catalase <ul><li>Enzyme that breaks down hydrogen peroxide (H 2 O 2 ), which is a naturally occurring poison. </li></ul><ul><li>The reaction catalyze by catalase is: </li></ul><ul><li>2H 2 O 2  2H 2 O + O 2 </li></ul><ul><li>When you put hydrogen peroxide on a cut, what are the bubbles? </li></ul><ul><li>Oxygen </li></ul>
    5. 5. Complete this sentence… <ul><li>The _____________ (more/less) enzyme, the faster the product is made. </li></ul>
    6. 6. The Procedure <ul><li>Part A: </li></ul><ul><li>Label 3 large test tubes hot, cold and room temp.  </li></ul><ul><li>Cut 3 1.5cm 3 pieces of potato with peel removed.  </li></ul><ul><li>Use a mortar and pestle to macerate (mash/grind up) each cube.  </li></ul><ul><li>Place ALL of the macerated potato cubes into each labeled test tube.  </li></ul>
    7. 7. <ul><li>6. Add 1ml of distilled water to each test tube containing potato mash.  </li></ul><ul><li>7. Place the test tube labeled hot in the hot water (100 o C) bath for 3 minutes. </li></ul><ul><li>8. Place the test tube labeled cold in the cold water bath (0-4 o C) for 3 minutes. </li></ul><ul><li>9. Leave the test tube labeled room temperature in the test tube rack.  </li></ul><ul><li>10. After 3 minutes, remove the test tube from the baths and allow the hot test tube to cool.   </li></ul>
    8. 8. <ul><li>11.  Add 5ml of H 2 O 2 (3.0% hydrogen peroxide) to each tube. </li></ul><ul><li>12. Wait one minute while reaction occurs then measure the height of the bubbles in each tube in cm. </li></ul><ul><li>13. Record in Table 1. Measure the radius of the test tube and record in Table 1. </li></ul><ul><li>14. Record your data in the class data table. </li></ul>
    9. 9. Teacher Role Student Role <ul><li>Safety </li></ul><ul><li>Read procedure to know what to do next </li></ul><ul><li>Follow the directions </li></ul><ul><li>Stay on task </li></ul><ul><li>Clean up </li></ul><ul><li>Safety </li></ul><ul><li>Have materials available </li></ul>

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