RT. Pawar et al., IJSID, 2012, 2 (5), 466-470                                                                             ...
RT. Pawar et al., IJSID, 2012, 2 (5), 466-470        Thus the host parasite relationship results in the gain of one organi...
RT. Pawar et al., IJSID, 2012, 2 (5), 466-470                                     Fig. I- T. S. of normal histological arc...
RT. Pawar et al., IJSID, 2012, 2 (5), 466-470         Fig. III & IV- T. S. of Infected intestine showing the penetration o...
RT. Pawar et al., IJSID, 2012, 2 (5), 466-470         The authors are thankful to the Head, Department of Zoology, Dr. Bab...
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Histopathology of intestinal tissue of mastacembelus armatus parasitized by ptychobothridae cestode parasites

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Histopathology of intestinal tissue of mastacembelus armatus parasitized by ptychobothridae cestode parasites

  1. 1. RT. Pawar et al., IJSID, 2012, 2 (5), 466-470 ISSN:2249-5347 IJSID International Journal of Science Innovations and Discoveries An International peer Review Journal for ScienceResearch Article Available online through www.ijsidonline.info HISTOPATHOLOGY OF INTESTINAL TISSUE OF MASTACEMBELUS ARMATUS PARASITIZED BY PTYCHOBOTHRIDAE CESTODE PARASITES Department of Zoology, Majalgaon Arts, Science and Commerce College, Majalgaon, Dist. Beed. (M.S.) India. R. T. PAWARReceived: 24-09-2012 ABSTRACT The present study communication deals with the study of histopathological changesAccepted: 14-10-2012 occurred in the intestinal tissue of Mastacembelus armatus collected from Marathwada region. The intestinal tissue recognized was parasitized by ptychobothridae cestode*Corresponding Author belonging to genus Senga Sp (Dollfus 1934). Here the cestode parasite having armed scolex adheres to intestinal tissue with the help of scolex and cause damages to villi, epithelium of host tissue. The worms are having pathogenic nature and are harmful to fishes and have got importance in aquaculture point of view i.e. the fish disease management side.Address: Key words: Ptychobothridae Cestode parasites, Mastacembelu armatus, Senga andName: Histopathology.RT. PawarPlace: INTRODUCTIONMajalgaon Arts Science andCommerce College, Majalgaon,MS, IndiaE-mail:drrajpawar@rediffmail.com INTRODUCTION International Journal of Science Innovations and Discoveries, Volume 2, Issue 5, September-October 2012 466
  2. 2. RT. Pawar et al., IJSID, 2012, 2 (5), 466-470 Thus the host parasite relationship results in the gain of one organism and the loss of another and leads to various INTRODUCTIONdiseases and disorders. Naturally it is important to study this relationship, not because of their parasitological value but for therelative existence of mankind. These studies may have considerable intrinsic interest and raise fundamental questions,common to other areas of biology, at a molecular, cellular, tissue and whole organism level. The host parasite relationship in cestodes is complex one, involving interactions between at least two or moregenetically systems, namely those of the parasite, its intermediate and its definitive host. Thus if a cestode, has to survive, mustbe suitably adopted to the morphology, physiology, biochemistry, immunology and ecology of its hosts. Review on the pathogenesis of adult cestodes has been made by Rees, G. (1967). There is also extensive literature onthe pathogenesis of larval cestodes in fish, but little is known of the reactions in the invertebrate’s hosts, and many adultparasite tapeworms have been studied, for their histopathology. For example in Pseudophylleidea, Bothriocephalusgowkongensis was studied by Kortings, (1977). In fishes Mcvicar (1972) described host parasite relationship ofEcheneibothrium, Phyllobothrium and Acanthobothrium. Sircar and Sinha (1980) have also studied the histopathology ofLytocestus indicus. Never the less quit of few reports on the pathogenicity of cestode on fishes are available i. e (Sindernann 1970, Bylund 1972).In this fist investigation we studied the histopathology of fish by cestode from Mastacembeleus armatus. For the histopathological study, the freshwater fish, Mastacembelus armatus were brought to the laboratory and MATERIALS AND METHODSdissected out the intestine. Some of the intestines were infected and some were not. The worms were collected washed insaline solution, flattened, preserved in 4% formalin and later processed for taxonomical studies. The infected intestine withcestode parasites on closer observation identified as genus Senga Sp (Dollfus 1934). The worms, which were attached tointestine, were kept intact and small pieces of such intestines and other healthy intestine were fixed in Bouin’s fluid fixative.Fixed tissues were washed, dehydrated through alcoholic grades, cleared in xylene and embedded in paraffin wax with meltingpoint (58-60oC). Blocks were cut at 8mµ and slides were stained with Haematoxylin: eosin stain. Best slides were selected andobserved under the microscope for histopathological study. The normal histological structure (Healthy intestine) of the host Mastacembelus armatus showed that the healthy villi RESULTS AND DISCUSSIONand all layers i.e. serosa, muscularis mucosa, submucosa and mucosa are clearly observed (Fig. I). The histopathology of infected intestine with the cestode Senga Sp. is showing penetrative type of scolex, invadesthrough the tissues and cause damages to villi, epithelium of host tissue. In the longitudinal section seen that the worm areapproaching to the intestinal villi. The mucosal layer of intestine and invades the host tissue, the worm is not only successful toenter into the intestine forming the ulceration in the intestinal wall; causing damage to the host tissue (Fig. II). The parasiteswere either free in the intestinal lumen of mixed with necrotic debris consisting of mucosal epithelium and monocular cells orembedded in the infesting of fibrocytes, lymphocytes, plasma cells and macrophages. International Journal of Science Innovations and Discoveries, Volume 2, Issue 5, September-October 2012 467
  3. 3. RT. Pawar et al., IJSID, 2012, 2 (5), 466-470 Fig. I- T. S. of normal histological architecture of the host M. armatus Fig. III & IV showing the magnified transverse section of penetration of parasites up to muscularis layer and entangledscolices. Fig. II- L.S. of infected intestine showing damaged area, penetrative type of scolex and adhered of scolices. International Journal of Science Innovations and Discoveries, Volume 2, Issue 5, September-October 2012 468
  4. 4. RT. Pawar et al., IJSID, 2012, 2 (5), 466-470 Fig. III & IV- T. S. of Infected intestine showing the penetration of parasites and entangled scolices. (Magnified) It is essential to study in detail the hosts nutrition other aspects in relation to parasitic interaction but from above itcan be concluded that the worms, Senga Sp. finds the food material and other favorable necessary requirement for itsnourishment and growth from the host tissue by causing damage to the intestinal tissue of host. This result is in accordancewith the Mackiewicz (1972). However, the helminthes crosses majority of the intestinal layers (internal epithelium,submucosa,muscularis layer) and come to lie near serosa suggesting that, it is very dangerous and destructive parasites to the The worms are having pathogenic nature and are harmful to fishes and have got importance in aquaculture point ofdefinitive host (C. J. Hiware,2008 )view i.e. the fish disease management side. International Journal of Science Innovations and Discoveries, Volume 2, Issue 5, September-October 2012 469
  5. 5. RT. Pawar et al., IJSID, 2012, 2 (5), 466-470 The authors are thankful to the Head, Department of Zoology, Dr. Babasaheb Ambedkar Marathwada University, AcknowledgementAurangabad, India for providing necessary laboratory and library facilities during tenure of this work.1. Bylund ,G (1972) : Pathogenic effects of Diphyllabathriid plerocercoid on its host fishes commentationes biologicae, REFERENCES societies scientatium fennica 58.1-112. Dollfus (1934) Sur uncestode Pseudophyllidae parasite de poisson ornament Bull. Sac. Zool. France 69; 476-490.3. Mackiewicz, J.S. (1972): Relationship of pathology of scolex morphology among caryophyllid cestodes. F. Parasitkde, 39(3): 232-246.4. Mcvicar, A.H. (1972): The ultra-structure of the parasite host interface of three tetraphyllidean tapeworms of the elasmobranch, Raji naevus. Parasitology, 65(1): 77-88.5. Rees, G. (1967): Pathogenesis of adult cestodes. Helm. Abst. , 36: 1-23.6. Sindermann C.J. (1970) :principal diseases of marine fishes and shellfish academic press New York 369 pp.7. Sircar, M. and Sinha, D.P. (1980): Histomorphology of Lytocestus indicus infection in the fish Clarias batrachus. Indian Soc. J. Anim. Res., 14(1): 53-56.8. Hiware C.J & Pawar R.T., (2008): Studies on Histopathology of Clarias batrachus (Linnaeus) Intestine Parasited by Cestode, Lytocesus clariasae Jadhav and Gahvane, 1991Journal of Yala Rajabhat University International Journal of Science Innovations and Discoveries, Volume 2, Issue 5, September-October 2012 470

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