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Heat induced differential proteomic changes reveal molecular mechanisms responsible for heat tolerance in chickpea
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Heat induced differential proteomic changes reveal molecular mechanisms responsible for heat tolerance in chickpea

  1. Feb 2017 Heat induced differential proteomic changes reveal molecular mechanisms responsible for heat tolerance in chickpea About ICRISAT: www.icrisat.org ICRISAT’s scientific information: http://EXPLOREit.icrisat.org *Correspondence: S.Parankusam@cgiar.org/santhikinnu@gmail.com Parankusam Santisree*, Pooja Bhatnagar-Mathur and Kiran K Sharma International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, Hyderabad-502324, Telangana, India Introduction Understanding the molecular differences in plant genotypes contrasting for heat sensitivity can provide useful insights into the mechanisms that confer heat tolerance in plants. This study focuses on comparative physiological and proteomic analyses of heat-sensitive (ICC16374) and heat-tolerant (JG14) genotypes of chickpea (Cicer arietinum L.) under heat stress impositions at anthesis. Under the current scenario of climate change, this study is important to enhance our understanding of the adaptation strategies in chickpea genotypes. Results Objectives Conclusions Summary of the heat-responsive proteins in chickpea. Sensitive(a) Control 136 HS 157 270 -153 +117 Tolerant(b) Control 145 HS 103 363 -221 +142 Sensitive 199 Tolerent 160 267 + 155 -112 (a) (b) (c) Fold change (LOG2) -4 -2 0 2 4 6 Heat shock protein LEA Sucrose synthase PAL2 RuBisCO GAPDH Aspartic proteinase SAMS ß-galactosidase Protein mRNA Enhanced membrane protection Accumulation of defense proteins Nucleic acid and ribosome synthesis Protection of protein homoeostasis Enhanced antioxidant defense ROS detoxification Increased sugars and aminoacids Osmoprotection Induction of phytohormones and signal transduction Induction of HSP’S Heat Stress Continued membrane transport Protection of cellular structures and synthesis Protection of Metabolism Enhanced heat adaptation Increased pod set Enhanced yield Enhanced CO2 assimilation Synthesis of secondary metabolites Chromatin modification & transcription The correlation of mRNA and protein expression levels of selected DEPs Schematic representation of the heat mitigation strategies in the tolerant genotype of chickpea Temperature (42 o C) Relativewatercontent(%) 0 20 40 60 80 100 Sensitive Tolerant Chl a Chl b Chl a+b Cholorophyll(mg/gFW) 0 5 10 15 20 25 30 Sensitive Tolerant Temperature (42o C) Electrolyteleakage(%) 0 2 4 6 8 10 12 14 16 18 Sensitive Tolerant Temperature ( o C) MDA(nmol/mL/gFW) 0 5 10 15 20 25 Sensitive Tolerant 27 42 (a) (b) (c) (d) * * * 27 42 TAC(AU) 0 20 40 60 80 100 Sensitive Tolerant 27 42 Solublesugars(mg.g-1FW) 0 1 2 3 4 5 6 Sensitive Tolerant 27 42 Temperature (°C) Temperature (°C) Temperature (°C)Temperature (°C) H2O2(mmol/mgprotein) 0 1 2 3 4 Sensitive Tolerant Proline(mmol/mgprotein) 0 2 4 6 8 10 12 Sensitive Tolerant 27 42 (a) (b) (c) (d) * * * * Effect of heat stress on physiological and biochemical parameters in contrasting chickpea genotypes. Comparative proteomics Identifying signaling partners NO as seed priming & foliar spray Stress treatment Understanding the induced tolerance by nitric oxide by priming and proteomics To develop the stress specific and genotype specific protein blueprints of chickpea Heat + Drought Salt Drought Way Forward Further studies will be on the functional characterization, regulation and post-translational modifications of the candidate proteins • Understanding the protein profile changes in the tolerant genotype under stress • Inducing the stress tolerance in sensitive genotype by exogenous NO application • This study identified a set of 363 & 270 heat responsive proteins in the sensitive and tolerant genotype respectively using comparative gel-free proteomics. • The proteins which are essentially related to the electron transport chain in photosynthesis, aminoacid biosynthesis, ribosome synthesis and secondary metabolite synthesis may play key roles in inducing heat tolerance. • This study also provides evidence that the foliar application of nitric oxide (NO) donor can enhance stress tolerance by modulating a number of proteins in chickpea. Acknowlegements This work was supported by INSPIRE Faculty Award (IFA12-LSPA-08) from the DST (India) and CGIAR Research Program on Grain Legumes.
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